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Metabacillus harenarius sp. nov., an alginate lyase-producing bacterium, and reclassification of 'Bacillus weihaiensis'.

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Τίτλος: Metabacillus harenarius sp. nov., an alginate lyase-producing bacterium, and reclassification of 'Bacillus weihaiensis'.
Συγγραφείς: Liang R; Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning 530006, PR China.; Institute of Tropical Bioscience and Biotechnology & Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101/Sanya 572024, PR China., Mo K; Institute of Tropical Bioscience and Biotechnology & Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101/Sanya 572024, PR China.; Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Haikou 571101, PR China., Zhao Z; Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning 530006, PR China.; Institute of Tropical Bioscience and Biotechnology & Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101/Sanya 572024, PR China., Hu Y; Institute of Tropical Bioscience and Biotechnology & Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101/Sanya 572024, PR China.; Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Haikou 571101, PR China.; Zhanjiang Experimental Station, CATAS, Zhanjiang, 524013, PR China., Zhang Z; Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning 530006, PR China., Jiang M; Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning 530006, PR China., Huang H; Institute of Tropical Bioscience and Biotechnology & Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101/Sanya 572024, PR China.; Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Haikou 571101, PR China.
Πηγή: International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2026 Jun; Vol. 76 (6).
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Microbiology Society Country of Publication: England NLM ID: 100899600 Publication Model: Print Cited Medium: Internet ISSN: 1466-5034 (Electronic) Linking ISSN: 14665026 NLM ISO Abbreviation: Int J Syst Evol Microbiol Subsets: MEDLINE
Imprint Name(s): Publication: 2015- : London : Microbiology Society
Original Publication: Reading, UK : Society for General Microbiology, c2000-
Ιατρικοί όροι (MeSH): Polysaccharide-Lyases*/metabolism , Bacillaceae*/classification , Bacillaceae*/genetics , Bacillaceae*/isolation & purification , Phylogeny* , Soil Microbiology*, RNA, Ribosomal, 16S/genetics ; Fatty Acids/chemistry ; DNA, Bacterial/genetics ; Phospholipids/chemistry ; Vitamin K 2/analogs & derivatives ; Vitamin K 2/chemistry ; Sand/microbiology ; Bacillus/classification ; Bacillus/genetics ; Bacterial Typing Techniques ; Base Composition ; Sequence Analysis, DNA ; China ; Nucleic Acid Hybridization
Περίληψη: A Gram-stain-negative, motile, rod-shaped, alginate lyase-producing bacterium, designated HB246100T, was isolated from coastal sand sample collected from Luhuitou Scenic Area, Sanya, China. The strain grew at 15-50 ℃ (optimum, 35 ℃), pH 6.0-11.0 (optimal, pH 8.0) and 0-10% (w/v) NaCl (optimum, 3%). The predominant isoprenoid quinone was found to be menaquinone 7, and the major fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C14 : 0 and iso-C16 : 0. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, an unidentified aminophospholipid, an unidentified lipid, three unidentified phospholipids and three unidentified glycolipids. The size of the genome was 4.29 Mbp, with a G+C content of 36.5 mol%. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain HB246100T belonged to the genus Metabacillus, and the closest phylogenetically related type species were Metabacillus crassostreae JSM 100118T, Metabacillus schmidteae Marseille-P9898T, Metabacillus halosaccharovorans E33T and Metabacillus litoralis SW-211T with the identities of 98.9, 98.1, 98.0 and 98.0%, respectively. The phylogenetic tree based on 16S rRNA gene sequences and the phylogenomic tree based on core genome sequences indicated that the novel strain was most closely related to M. crassostreae and M. litoralis, with average nucleotide identity of 74.8-75.6% and digital DNA-DNA hybridization value of 22.1-22.4%. Comprehensive phenotypic, phylogenetic and genomic characteristics demonstrate that strain HB246100T is distinct from the species with validly published names in the genus Metabacillus, suggesting that it represents a novel species of the genus Metabacillus and proposing the name Metabacillus harenarius sp. nov. The type strain is HB246100T (=MCCC 1K09923T=JCM 38130T). Furthermore, 'Bacillus weihaiensis' Alg07, which only publicly disclosed its genome sequence, was emended and reclassified as M. harenarius.
Contributed Indexing: Keywords: 'Bacillus weihaiensis'; Metabacillus harenarius sp. nov.; alginate lyase; coastal sand
Substance Nomenclature: 0 (RNA, Ribosomal, 16S)
0 (Fatty Acids)
EC 4.2.2.- (Polysaccharide-Lyases)
0 (DNA, Bacterial)
EC 4.2.2.3 (poly(beta-D-mannuronate) lyase)
0 (Phospholipids)
11032-49-8 (Vitamin K 2)
0 (Sand)
8427BML8NY (menaquinone 7)
Entry Date(s): Date Created: 20260601 Date Completed: 20260717 Latest Revision: 20260717
Update Code: 20260717
DOI: 10.1099/ijsem.0.007185
PMID: 42223994
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1466-5034
DOI:10.1099/ijsem.0.007185