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Genetic diversity and population structure of Passiflora spp. using inter-primer binding site (iPBS) - retrotransposon markers.

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Τίτλος: Genetic diversity and population structure of Passiflora spp. using inter-primer binding site (iPBS) - retrotransposon markers.
Συγγραφείς: de Jesus ON; Embrapa Mandioca e Fruticultura, Rua Embrapa, s/n, Cruz das Almas, P.O. Box 007, Chapadinha, Bahia, 44380-000, Brazil. onildo.nunes@embrapa.br., Costa EMR; Embrapa Mandioca e Fruticultura, Rua Embrapa, s/n, Cruz das Almas, P.O. Box 007, Chapadinha, Bahia, 44380-000, Brazil., Azevedo FQ; Embrapa Clima Temperado, BR 392, km 78 - 9º Distrito, Monte Bonito, P.O. Box 403, Pelotas, 96010-971, Rio Grande do Sul, Brazil., de Sousa VR; Embrapa Mandioca e Fruticultura, Rua Embrapa, s/n, Cruz das Almas, P.O. Box 007, Chapadinha, Bahia, 44380-000, Brazil., Gonçalves ZS; Embrapa Mandioca e Fruticultura, Rua Embrapa, s/n, Cruz das Almas, P.O. Box 007, Chapadinha, Bahia, 44380-000, Brazil., Lima LKS; Universidade Estadual de Feira de Santana, Av. Transnordestina, s/n, Novo Horizonte, Feira de Santana, Bahia, 44036-900, Brazil., Soares TL; Embrapa Mandioca e Fruticultura, Rua Embrapa, s/n, Cruz das Almas, P.O. Box 007, Chapadinha, Bahia, 44380-000, Brazil.
Πηγή: Molecular biology reports [Mol Biol Rep] 2026 Jun 01; Vol. 53 (1). Date of Electronic Publication: 2026 Jun 01.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Reidel Country of Publication: Netherlands NLM ID: 0403234 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-4978 (Electronic) Linking ISSN: 03014851 NLM ISO Abbreviation: Mol Biol Rep Subsets: MEDLINE
Imprint Name(s): Original Publication: Dordrecht, Boston, Reidel.
Ιατρικοί όροι (MeSH): Passiflora*/genetics , Genetic Variation*/genetics , Retroelements*/genetics, Genetics, Population/methods ; Binding Sites/genetics ; Genetic Markers/genetics ; DNA Primers/genetics ; Genotype ; Phylogeny ; Polymorphism, Genetic ; Alleles ; Principal Component Analysis ; Cluster Analysis
Περίληψη: Background: The Passiflora genus presents a representative variability of passion fruit species with food, pharmacological and ornamental potential. To understand this genetic variability, it is essential that genetic resources are well characterized for future use in breeding and conservation programs. The aim of this study was to investigate for the first time the diversity genetic and structure population of 88 Passiflora genotypes with the use of inter-primer binding site (iPBS) markers.
Methods and Results: Genotypes were grouped through Unweighted Pair-Group Mean Average (UPGMA). The population structure analysis was carried out by two methods using model-based Structure analysis and cluster-based discriminant analysis of principal components (DAPC). Samples of 88 genotypes were analyzed using 24 iPBS markers, of which 19 of them generated 234 informative bands, with an average of 12.32 per locus, ranging from 5 to 18 alleles. Polymorphic information content (PIC) values varied between 0.14 and 0.43, with an average value of 0.28. Nei's genetic diversity (He) as 0.28 and Shannon's information index (I) as 0.43. The genetic dissimarity for the 88 Passiflora genotypes ranged from 0.11 to 0.97, with mean values of 0.74, indicating substantial genetic diversity. Molecular variance analysis (AMOVA) indicated that within group variations contributed more (51%) to the genetic diversity than among group variations (49%), with percetage of the genetic differentiation coefficient (FST= 0.49). Discriminant analysis of principal components (DAPC) corroborated by cluster analysis (UPGMA) and Bayesian model-based analysis of population genetic structure categorized all materials into ten groups. Notably, a substantial portion of genotypes clustered within populations correlated with the Passiflora species. The ornamental hybrids were all discriminated based on the iPBS markers used and the G9 group allowed for the allocation of at least six of them.
Conclusions: The results of this study provide valuable information for passion fruit germplasm management and contribute to the improvement of current breeding strategies. They also demonstrated the effectiveness of iPBS retrotransposon markers in detecting genetic differentiation and reliably elucidating genetic relationships among passion fruit genotypes, thereby supporting breeding programs and germplasm conservation efforts.
(© 2026. The Author(s).)
Competing Interests: Declarations. Competing interests: The authors declare no competing interests. Ethical approval: This article does not contain any studies with human participants or animals performed by any of the authors. Consent to publish: All authors read the manuscript and showed their willingness to publish this study.
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Contributed Indexing: Keywords: Genetic relationship; Genetic resources; Passiflora; iPBS markers
Substance Nomenclature: 0 (Retroelements)
0 (Genetic Markers)
0 (DNA Primers)
Entry Date(s): Date Created: 20260601 Date Completed: 20260717 Latest Revision: 20260726
Update Code: 20260726
PubMed Central ID: PMC13226455
DOI: 10.1007/s11033-026-12038-9
PMID: 42223718
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1573-4978
DOI:10.1007/s11033-026-12038-9