Characterization and mechanism probe of enzymatic extraction and thiolation modification of wheat bran arabinoxylan.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Characterization and mechanism probe of enzymatic extraction and thiolation modification of wheat bran arabinoxylan.
Συγγραφείς: Jin C; National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China., Zhu L; National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Shandong Key Laboratory of Food Resources and Healthy, Shandong 256600, China. Electronic address: zhuling@jiangnan.edu.cn., Tang J; National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China., Xiao Y; National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China., Wan J; National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China., Zhang H; National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Shandong Key Laboratory of Food Resources and Healthy, Shandong 256600, China. Electronic address: zhanghui@jiangnan.edu.cn.
Πηγή: Food research international (Ottawa, Ont.) [Food Res Int] 2026 Aug 31; Vol. 238, pp. 119424. Date of Electronic Publication: 2026 May 10.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science Country of Publication: Canada NLM ID: 9210143 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7145 (Electronic) Linking ISSN: 09639969 NLM ISO Abbreviation: Food Res Int Subsets: MEDLINE
Imprint Name(s): Original Publication: Ottawa, Ontario, Canada : Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science, c1992-
Ιατρικοί όροι (MeSH): Xylans*/chemistry , Xylans*/isolation & purification , Sulfhydryl Compounds*/chemistry , Dietary Fiber*/analysis, Antioxidants/analysis ; Antioxidants/chemistry ; Hydrophobic and Hydrophilic Interactions ; Spectroscopy, Fourier Transform Infrared
Περίληψη: This study successfully extracted arabinoxylan (AX) from wheat bran employing enzymatic and graded alcohol precipitation methods, and subsequently thiolated via an amidation grafting reaction with cysteine. AX extracted from extruded wheat bran by 80% (v/v) ethanol precipitation (E-AX80) has high purity (69.64%), uronic acid (2.47%) and FA content (7.23 μg/mg). Thiolated modified AX (E-AX@SH) was determined to contain 134.32-349.76 μmol/g of free sulfydryl (-SH) via Ellman method. Elemental analysis, molecular dynamics simulation, FTIR, and NMR were employed to validate the successful modification and inter/intra-molecular chemical interactions of E-AX@SH (e.g., hydrogen bonds, hydrophobic interactions, and disulfide bonds). Structural analyses (XRD, SAXS, SEM, AFM, and fluorescence spectroscopy) revealed that modification induced a configurational rearrangement of E-AX@SH molecule, leading to a more compact structure, a rougher surface, and a more hydrophobic internal microenvironment. The antioxidant capacity of E-AX@SH was remarkably higher than E-AX80. Furthermore, rheological behavior and Raman spectroscopy demonstrated that E-AX@SH forms disulfide bonds with intestinal mucus to construct a robust viscoelastic network, exhibiting outstanding mucosal adhesion properties. This work presents a noval perspective for the high-value utilization of wheat bran and the construction of targeted carriers.
(Copyright © 2026. Published by Elsevier Ltd.)
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Contributed Indexing: Keywords: Adhesion properties; Antioxidant activity; Molecular configuration; Thiolated modification; Wheat bran arabinoxylan (WBAX)
Substance Nomenclature: 0 (Xylans)
9040-27-1 (arabinoxylan)
0 (Sulfhydryl Compounds)
0 (Dietary Fiber)
0 (Antioxidants)
Entry Date(s): Date Created: 20260529 Date Completed: 20260717 Latest Revision: 20260717
Update Code: 20260717
DOI: 10.1016/j.foodres.2026.119424
PMID: 42215092
Βάση Δεδομένων: MEDLINE