Synergistic antibiotic-laden wastewater treatment doubles denitrification rate in a pilot mineral-based autotrophic biofilter by breaking microbial spatial-metabolic constraints.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	Synergistic antibiotic-laden wastewater treatment doubles denitrification rate in a pilot mineral-based autotrophic biofilter by breaking microbial spatial-metabolic constraints.
Συγγραφείς:	Zhang Y; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing, 100124, China. Electronic address: 3167353554@qq.com., Zhang L; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing, 100124, China. Electronic address: zhangli19821115a@163.com., Zhang S; Research and Development Center of Beijing Drainage Group Co. Ltd, Beijing, 100124, China. Electronic address: 13810812776@163.com., Yang C; Guangdong Beijing Enterprises Shixi Technology Co., Ltd, Guangdong, 511300, China. Electronic address: 1131474646@qq.com., Wang Z; Research and Development Center of Beijing Drainage Group Co. Ltd, Beijing, 100124, China. Electronic address: 18701060300@163.com., Si G; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing, 100124, China. Electronic address: sgc1996@126.com., Peng Y; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing, 100124, China. Electronic address: pyz@bjut.edu.cn.
Πηγή:	Environmental research [Environ Res] 2026 Aug 15; Vol. 304, pp. 124860. Date of Electronic Publication: 2026 May 28.
Τύπος έκδοσης:	Journal Article
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0147621 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0953 (Electronic) Linking ISSN: 00139351 NLM ISO Abbreviation: Environ Res Subsets: MEDLINE
Imprint Name(s):	Publication: <2000- > : Amsterdam : Elsevier Original Publication: New York, Academic Press.
Ιατρικοί όροι (MeSH):	Wastewater/chemistry , Wastewater/microbiology , Waste Disposal, Fluid/methods , Water Pollutants, Chemical/metabolism , Bioreactors/microbiology , Denitrification , Anti-Bacterial Agents*, Bacteria/metabolism ; Autotrophic Processes ; Sulfides ; Pilot Projects ; Iron ; Filtration
Περίληψη:	The practical application of pyrite-based autotrophic denitrification biofilters (PADB) is limited by their low nitrogen removal rate (NRR). This study demonstrates that in a pilot-scale PADB (750 L) treating NO3^--N wastewater, heterotrophic bacterial consortia (HBs) severely impair the denitrification activity of autotrophic denitrifying bacteria (ADB) through network encapsulation and metabolic shunting. However, after switching to treating composite wastewater containing antibiotics, the system achieved an antibiotic removal rate of 95.12%. And it's NRR from 32.37 to 63.15 mg N/(L·d), representing a 0.95-fold enhancement. Integrated co-occurrence network and metagenomic analyses revealed a three-stage cascade reaction underlying this improvement: (i) The antibiotic stress halted carbon-feeding from ADB and hydrolytic-acidifying bacteria to HBs (fermentation gene abundance decreased by 1.89-58.45%), depriving HBs of energetic and substrate support and resulting in their selective elimination (0.63-fold decrease in relative abundance). This relieved ADB's metabolic burden and shortened their physical distance to pyrite; (ii) Elevated electron and energy demand in ADB activated dormant genes for electron shuttle synthesis (menC/E: 0 to 342/402 TPM) and upregulated sulfur metabolism genes (∼3.9-fold), enhancing pyrite dissolution and electron harvesting; (iii) This augmented electron flow stimulated ADB's carbon fixation pathway (Calvin-Benson-Bassham cycle genes upregulated 14.89-fold) and amplified energy metabolism (1.33-1.55-fold enhancement in glycolysis and Tricarboxylic Acid cycle), supplying ample material and energy for ADB proliferation and denitrification. Consequently, ADB enrichment accelerated 509-fold, while the abundance of key denitrification genes (napA/B, nosZ) increased by 2.1-11.04-fold. These molecular and population-level changes doubled the system's NRR compared to its original level. (Copyright © 2026 Elsevier Inc. All rights reserved.)
Competing Interests:	Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Contributed Indexing:	Keywords: Antibiotic; Autotrophic denitrification; Metagenomic analysis; Pilot-scale reactor; Pyrite
Substance Nomenclature:	0 (Wastewater) 0 (Anti-Bacterial Agents) 0 (Sulfides) 0 (Water Pollutants, Chemical) 132N09W4PR (pyrite) E1UOL152H7 (Iron)
Entry Date(s):	Date Created: 20260529 Date Completed: 20260611 Latest Revision: 20260611
Update Code:	20260611
DOI:	10.1016/j.envres.2026.124860
PMID:	42214594
Βάση Δεδομένων:	MEDLINE

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