Academic Journal
Assay-dependent discordance in detecting a rare HLA-DPB1 allele: Long-range PCR vs. hybrid capture NGS.
| Τίτλος: | Assay-dependent discordance in detecting a rare HLA-DPB1 allele: Long-range PCR vs. hybrid capture NGS. |
|---|---|
| Συγγραφείς: | Rozar K; HLA Laboratory, Moffitt Cancer Center, 10902 N. McKinley Drive, Tampa, FL 33612, United States. Electronic address: katie.rozar@moffitt.org., Cano P; HLA Laboratory, Moffitt Cancer Center, 10902 N. McKinley Drive, Tampa, FL 33612, United States. Electronic address: pedro.cano@moffitt.org., Verma D; Clinical Testing Development, Moffitt Cancer Center, 10902 N. McKinley Drive, Tampa, FL 33612, United States. Electronic address: deepak.verma@moffitt.org., Wendel J; HLA Laboratory, Moffitt Cancer Center, 10902 N. McKinley Drive, Tampa, FL 33612, United States. Electronic address: jennifer.wendel@moffitt.org., Iozzino C; HLA Laboratory, Moffitt Cancer Center, 10902 N. McKinley Drive, Tampa, FL 33612, United States. Electronic address: crystal.iozzino@moffitt.org. |
| Πηγή: | Human immunology [Hum Immunol] 2026 Aug; Vol. 87 (8), pp. 111769. Date of Electronic Publication: 2026 May 29. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier/North-Holland Country of Publication: United States NLM ID: 8010936 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1166 (Electronic) Linking ISSN: 01988859 NLM ISO Abbreviation: Hum Immunol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: [New York] Elsevier/North-Holland. |
| Ιατρικοί όροι (MeSH): | Polymerase Chain Reaction*/methods , Histocompatibility Testing*/methods , High-Throughput Nucleotide Sequencing*/methods , HLA-DP beta-Chains*/genetics, Humans ; Alleles |
| Περίληψη: | Next-generation sequencing (NGS) has become fundamental to high-resolution HLA typing, but methodological differences between long-range PCR (LR PCR) and hybrid capture (HC) approaches can lead to discrepant results. This study examined cases where an allele identified as DPB1*13:01 by LR PCR was assigned DPB1*107:01 by HC. This discordance was attributed to both primer-site mutations and incomplete coverage of non-coding regions in LR PCR, resulting in allele dropout and preferential amplification. Conversely, typing by HC captures full genomic regions and minimizes PCR-generated errors, enabling accurate allele assignment. Findings indicate that DPB1*107:01 may be more prevalent than current CIWD classifications suggest, while broader implications include the need for proficiency testing programs to incorporate method-specific grading criteria that reflect the diversity of available typing technologies. This study underscores the importance of comprehensive genomic coverage for accurate HLA typing and supports the expanded adoption of HC-based sequencing in clinical laboratories. (Copyright © 2026 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: DPB1*107:01; HLA typing; NGS; hybrid capture; long-range PCR |
| Substance Nomenclature: | 0 (HLA-DP beta-Chains) 0 (HLA-DPB1 antigen) |
| Entry Date(s): | Date Created: 20260529 Date Completed: 20260721 Latest Revision: 20260721 |
| Update Code: | 20260722 |
| DOI: | 10.1016/j.humimm.2026.111769 |
| PMID: | 42214264 |
| Βάση Δεδομένων: | MEDLINE |
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