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Simplified Derivation of Stable Early Passage Murine Embryonic and Parthenogenetic Stem Cells.

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Title: Simplified Derivation of Stable Early Passage Murine Embryonic and Parthenogenetic Stem Cells.
Authors: Pery E; Bedford Research Foundation, Bedford, Massachusetts, USA., Gervasi MG; Department of Animal Science, University of Connecticut, Storrs, Connecticut, USA., Hauser A; Bedford Research Foundation, Bedford, Massachusetts, USA., Leppanen S; Agilent Technologies, Santa Clara, California, USA., Kiessling AA; Bedford Research Foundation, Bedford, Massachusetts, USA.
Source: Stem cells and development [Stem Cells Dev] 2026 Jun; Vol. 35 (11-12), pp. 222-231. Date of Electronic Publication: 2026 May 27.
Publication Type: Journal Article
Language: English
Journal Info: Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 101197107 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-8534 (Electronic) Linking ISSN: 15473287 NLM ISO Abbreviation: Stem Cells Dev Subsets: MEDLINE
Imprint Name(s): Original Publication: Larchmont, NY : Mary Ann Liebert, Inc., c2004-
MeSH Terms: Embryonic Stem Cells*/cytology , Embryonic Stem Cells*/metabolism , Mouse Embryonic Stem Cells*/cytology , Mouse Embryonic Stem Cells*/metabolism , Cell Culture Techniques*/methods , Parthenogenesis*, Animals ; Mice ; Female ; Comparative Genomic Hybridization ; Male ; Aneuploidy ; Cell Line
Abstract: Aneuploidy in cultured embryonic stem (ES) cells severely limits research and therapeutic potential. To begin to understand when chromosomal imbalances arise, primary ES cell lines were derived in feeder-free, fully defined culture media from 44% of two-cell CF1 embryos (5 lines), 37% of CF1 multi-drug resistance (Mdr1/Abcb1) mutant embryos (19 lines), and 2 parthenogenetic stem (mPS) lines from Mdr1 mutant eggs for array comparative genomic hybridization analysis. By passage 2, trivial somatic chromosome euploidy was detected in all wild-type lines and in 9 (64%) of the 14 mutant cell lines. Five Mdr1 mutant ES cell lines and both mPS cell lines exhibited mosaic gain of chromosome 8 in at least 30% of cells, a common finding in mouse ES cells. X chromosomes exhibited more aneuploidy than somatic chromosomes. Five of the Mdr1 mutant lines were male, while the nine female ES cell lines and both mPS lines exhibited almost complete loss of one X chromosome. Two ES lines analyzed at passage 3 exhibited partial gains and losses in chromosomes 15 and 19, suggesting that aneuploidy in murine ES cells begins by passage 3 in standard culture conditions. The somatic cell euploidy in early passage wild-type CF1 ES lines, in contrast to the early accumulation of chromosome 8 triploidy in the Mdr1 mutant lines, suggests that component(s) of the culture system not well tolerated by ES cells with the Mdr1 mutation may be responsible for triploid chromosome 8 in multipassaged mouse ES cells. The ease of deriving euploid mouse stem cell lines in fully defined culture conditions from individual females encourages the use of primary ES cell lines for a variety of purposes. Adjusting culture components to correct early chromosome 8 accumulation in primary Mdr1 mutant lines may reveal pathways critical to maintaining euploidy in cultured ES cells.
Competing Interests: Author Disclosure StatementThe authors declare no competing interests.
Contributed Indexing: Keywords: array comparative genomic hybridization; euploidy and aneuploidy; mouse embryonic stem cells; parthenogenetic stem cells
Entry Date(s): Date Created: 20260527 Date Completed: 20260603 Latest Revision: 20260603
Update Code: 20260603
DOI: 10.1177/15473287261450907
PMID: 42200562
Database: MEDLINE
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  Data: Simplified Derivation of Stable Early Passage Murine Embryonic and Parthenogenetic Stem Cells.
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  Data: <searchLink fieldCode="AU" term="%22Pery+E%22">Pery E</searchLink>; Bedford Research Foundation, Bedford, Massachusetts, USA.<br /><searchLink fieldCode="AU" term="%22Gervasi+MG%22">Gervasi MG</searchLink>; Department of Animal Science, University of Connecticut, Storrs, Connecticut, USA.<br /><searchLink fieldCode="AU" term="%22Hauser+A%22">Hauser A</searchLink>; Bedford Research Foundation, Bedford, Massachusetts, USA.<br /><searchLink fieldCode="AU" term="%22Leppanen+S%22">Leppanen S</searchLink>; Agilent Technologies, Santa Clara, California, USA.<br /><searchLink fieldCode="AU" term="%22Kiessling+AA%22">Kiessling AA</searchLink>; Bedford Research Foundation, Bedford, Massachusetts, USA.
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  Data: <searchLink fieldCode="JN" term="%22101197107%22">Stem cells and development</searchLink> [Stem Cells Dev] 2026 Jun; Vol. 35 (11-12), pp. 222-231. <i>Date of Electronic Publication: </i>2026 May 27.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Mary+Ann+Liebert%2C+Inc%22">Mary Ann Liebert, Inc </searchLink><i>Country of Publication: </i>United States <i>NLM ID: </i>101197107 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1557-8534 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2215473287%22">15473287 </searchLink><i>NLM ISO Abbreviation: </i>Stem Cells Dev <i>Subsets: </i>MEDLINE
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  Data: <i>Original Publication</i>: Larchmont, NY : Mary Ann Liebert, Inc., c2004-
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  Data: <searchLink fieldCode="MM" term="%22Embryonic+Stem+Cells%22">Embryonic Stem Cells*</searchLink>/<searchLink fieldCode="MM" term="%22Embryonic+Stem+Cells+cytology%22">cytology</searchLink> <br /><searchLink fieldCode="MM" term="%22Embryonic+Stem+Cells%22">Embryonic Stem Cells*</searchLink>/<searchLink fieldCode="MM" term="%22Embryonic+Stem+Cells+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Mouse+Embryonic+Stem+Cells%22">Mouse Embryonic Stem Cells*</searchLink>/<searchLink fieldCode="MM" term="%22Mouse+Embryonic+Stem+Cells+cytology%22">cytology</searchLink> <br /><searchLink fieldCode="MM" term="%22Mouse+Embryonic+Stem+Cells%22">Mouse Embryonic Stem Cells*</searchLink>/<searchLink fieldCode="MM" term="%22Mouse+Embryonic+Stem+Cells+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Cell+Culture+Techniques%22">Cell Culture Techniques*</searchLink>/<searchLink fieldCode="MM" term="%22Cell+Culture+Techniques+methods%22">methods</searchLink> <br /><searchLink fieldCode="MM" term="%22Parthenogenesis%22">Parthenogenesis*</searchLink><br /><searchLink fieldCode="MH" term="%22Animals%22">Animals</searchLink> ; <searchLink fieldCode="MH" term="%22Mice%22">Mice</searchLink> ; <searchLink fieldCode="MH" term="%22Female%22">Female</searchLink> ; <searchLink fieldCode="MH" term="%22Comparative+Genomic+Hybridization%22">Comparative Genomic Hybridization</searchLink> ; <searchLink fieldCode="MH" term="%22Male%22">Male</searchLink> ; <searchLink fieldCode="MH" term="%22Aneuploidy%22">Aneuploidy</searchLink> ; <searchLink fieldCode="MH" term="%22Cell+Line%22">Cell Line</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Aneuploidy in cultured embryonic stem (ES) cells severely limits research and therapeutic potential. To begin to understand when chromosomal imbalances arise, primary ES cell lines were derived in feeder-free, fully defined culture media from 44% of two-cell CF1 embryos (5 lines), 37% of CF1 multi-drug resistance (Mdr1/Abcb1) mutant embryos (19 lines), and 2 parthenogenetic stem (mPS) lines from Mdr1 mutant eggs for array comparative genomic hybridization analysis. By passage 2, trivial somatic chromosome euploidy was detected in all wild-type lines and in 9 (64%) of the 14 mutant cell lines. Five Mdr1 mutant ES cell lines and both mPS cell lines exhibited mosaic gain of chromosome 8 in at least 30% of cells, a common finding in mouse ES cells. X chromosomes exhibited more aneuploidy than somatic chromosomes. Five of the Mdr1 mutant lines were male, while the nine female ES cell lines and both mPS lines exhibited almost complete loss of one X chromosome. Two ES lines analyzed at passage 3 exhibited partial gains and losses in chromosomes 15 and 19, suggesting that aneuploidy in murine ES cells begins by passage 3 in standard culture conditions. The somatic cell euploidy in early passage wild-type CF1 ES lines, in contrast to the early accumulation of chromosome 8 triploidy in the Mdr1 mutant lines, suggests that component(s) of the culture system not well tolerated by ES cells with the Mdr1 mutation may be responsible for triploid chromosome 8 in multipassaged mouse ES cells. The ease of deriving euploid mouse stem cell lines in fully defined culture conditions from individual females encourages the use of primary ES cell lines for a variety of purposes. Adjusting culture components to correct early chromosome 8 accumulation in primary Mdr1 mutant lines may reveal pathways critical to maintaining euploidy in cultured ES cells.
– Name: Abstract
  Label: Competing Interests
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  Data: Author Disclosure StatementThe authors declare no competing interests.
– Name: SubjectMinor
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  Data: <i>Keywords: </i>array comparative genomic hybridization; euploidy and aneuploidy; mouse embryonic stem cells; parthenogenetic stem cells
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  Data: <i>Date Created: </i>20260527 <i>Date Completed: </i>20260603 <i>Latest Revision: </i>20260603
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  Data: 10.1177/15473287261450907
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  Data: 42200562
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1177/15473287261450907
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        StartPage: 222
    Subjects:
      – SubjectFull: Animals
        Type: general
      – SubjectFull: Mice
        Type: general
      – SubjectFull: Female
        Type: general
      – SubjectFull: Comparative Genomic Hybridization
        Type: general
      – SubjectFull: Male
        Type: general
      – SubjectFull: Aneuploidy
        Type: general
      – SubjectFull: Cell Line
        Type: general
      – SubjectFull: Embryonic Stem Cells cytology
        Type: general
      – SubjectFull: Embryonic Stem Cells metabolism
        Type: general
      – SubjectFull: Mouse Embryonic Stem Cells cytology
        Type: general
      – SubjectFull: Mouse Embryonic Stem Cells metabolism
        Type: general
      – SubjectFull: Cell Culture Techniques methods
        Type: general
      – SubjectFull: Parthenogenesis
        Type: general
    Titles:
      – TitleFull: Simplified Derivation of Stable Early Passage Murine Embryonic and Parthenogenetic Stem Cells.
        Type: main
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            NameFull: Pery E
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            NameFull: Gervasi MG
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            NameFull: Hauser A
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            – D: 01
              M: 06
              Text: 2026 Jun
              Type: published
              Y: 2026
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              Value: 35
            – Type: issue
              Value: 11-12
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            – TitleFull: Stem cells and development
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