Academic Journal
Enhanced levansucrase expression in Bacillus subtilis via regulatory element optimization for high-yield lactosucrose production from sucrose and lactose.
| Τίτλος: | Enhanced levansucrase expression in Bacillus subtilis via regulatory element optimization for high-yield lactosucrose production from sucrose and lactose. |
|---|---|
| Συγγραφείς: | He H; State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China., Yang Y; Beijing Vocational College of Agriculture, Department of Food and Bioengineering, Beijing 102442, China., Wei Q; State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China., Liu X; Shandong Haizhibao Ocean Technology Co., Ltd, Weihai, Shandong 264333, China., Xu W; State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China., Zhang W; State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China., Ni D; State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Shandong Haizhibao Ocean Technology Co., Ltd, Weihai, Shandong 264333, China. Electronic address: daweini@jiangnan.edu.cn., Mu W; State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. |
| Πηγή: | Bioresource technology [Bioresour Technol] 2026 Sep; Vol. 456, pp. 134954. Date of Electronic Publication: 2026 May 22. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Applied Science Country of Publication: England NLM ID: 9889523 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2976 (Electronic) Linking ISSN: 09608524 NLM ISO Abbreviation: Bioresour Technol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991- |
| Ιατρικοί όροι (MeSH): | Bacillus subtilis*/genetics , Bacillus subtilis*/metabolism , Bacillus subtilis*/enzymology , Hexosyltransferases*/metabolism , Hexosyltransferases*/genetics , Sucrose*/metabolism , Trisaccharides*/biosynthesis , Lactose*/metabolism , Regulatory Sequences, Nucleic Acid*/genetics, Hydrogen-Ion Concentration ; Gene Expression Regulation, Bacterial ; Fermentation ; Temperature ; Promoter Regions, Genetic |
| Περίληψη: | Lactosucrose is a functional oligosaccharide with prebiotic properties and broad applications in the food and nutraceutical industries. In this study, a systematic strategy was developed to enhance lactosucrose production by optimizing levansucrase expression in Bacillus subtilis. Six levansucrases were screened, and levansucrase from Brenneria goodwinii (BgLS) exhibited the highest solubility and whole-cell catalytic activity. Whole-cell reaction conditions were optimized, identifying pH 6.0 and 50 °C as optimal. BgLS expression was further enhanced through translational regulation by engineering synthetic 5'-untranslated regions (5'-UTRs) and N-terminal coding sequences (NCSs), followed by combinatorial optimization. Subsequent transcriptional optimization using endogenous promoters further increased enzyme expression. The best engineered strain achieved a fermentation activity of 190.7 U/mL and produced lactosucrose with a 46.5% conversion rate from 200 g/L lactose and 300 g/L sucrose. This work demonstrates an efficient platform for high-level lactosucrose biosynthesis and highlights the effectiveness of multi-level gene expression regulation. (Copyright © 2026 Elsevier Ltd. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Bacillus subtilis; Lactosucrose; Levansucrase; Translational and transcriptional regulation; Whole-cellbiocatalysis |
| Substance Nomenclature: | EC 2.4.1.10 (levansucrase) EC 2.4.1.- (Hexosyltransferases) 57-50-1 (Sucrose) 0 (Trisaccharides) J2B2A4N98G (Lactose) |
| Entry Date(s): | Date Created: 20260523 Date Completed: 20260609 Latest Revision: 20260609 |
| Update Code: | 20260610 |
| DOI: | 10.1016/j.biortech.2026.134954 |
| PMID: | 42176820 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-2976 |
|---|---|
| DOI: | 10.1016/j.biortech.2026.134954 |