Academic Journal
Antibiofilm effect of Curcuma longa L. extract in antimicrobial photodynamic therapy against Enterococcus faecalis.
| Τίτλος: | Antibiofilm effect of Curcuma longa L. extract in antimicrobial photodynamic therapy against Enterococcus faecalis. |
|---|---|
| Συγγραφείς: | Cullash Luza ACH; Faculty of Dentistry, Universidad de San Martín de Porres (USMP), Lima, Peru. Electronic address: acullashl@usmp.pe., Salazar Salvatierra ME; Faculty of Pharmacy and Biochemistry, Universidad Nacional Mayor de San Marcos (UNMSM), Lima, Peru., Luza Montero SC; Faculty of Dentistry, Universidad Nacional Mayor de San Marcos (UNMSM), Lima, Peru., Castro Yanahida JE; Faculty of Dentistry, Universidad Nacional Mayor de San Marcos (UNMSM), Lima, Peru., Gutierrez Leiva AC; Faculty of Dentistry, Universidad Nacional Mayor de San Marcos (UNMSM), Lima, Peru., Temoche Rosales CA; Faculty of Dentistry, Universidad Nacional Mayor de San Marcos (UNMSM), Lima, Peru., Colchado Carhuavilca JR; Faculty of Dentistry, Universidad Nacional Mayor de San Marcos (UNMSM), Lima, Peru. |
| Πηγή: | Photodiagnosis and photodynamic therapy [Photodiagnosis Photodyn Ther] 2026 Jun; Vol. 59, pp. 105515. Date of Electronic Publication: 2026 May 15. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101226123 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-1597 (Electronic) Linking ISSN: 15721000 NLM ISO Abbreviation: Photodiagnosis Photodyn Ther Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam ; Boston : Elsevier, c2004- |
| Ιατρικοί όροι (MeSH): | Enterococcus faecalis*/drug effects , Biofilms*/drug effects , Plant Extracts*/pharmacology , Plant Extracts*/administration & dosage , Photochemotherapy*/methods , Curcuma*/chemistry , Photosensitizing Agents*/pharmacology , Photosensitizing Agents*/administration & dosage, Chlorhexidine/pharmacology ; Dose-Response Relationship, Drug |
| Περίληψη: | Background: Enterococcus faecalis is associated with persistent endodontic infections due to its ability to form resistant biofilms. Antimicrobial photodynamic therapy (aPDT) has emerged as a promising alternative approach. This study evaluated the antibiofilm activity of an ethanolic extract of Curcuma longa L. against E. faecalis ATCC 29,212, with and without aPDT. Methods: An in vitro biofilm model was used. The minimum biofilm inhibitory concentration (MBIC) was determined. Biofilms were treated with the C. longa extract (7.81-2000 µg/mL) under LED irradiation (aPDT) and without light activation. Chlorhexidine (2%) served as a positive control while untreated biofilms served as negative control. Biofilm biomass was quantified by optical density, and statistical analysis was performed using ANOVA and Tukey's test (p < 0.05). Results: The MBIC was 62.5 µg/mL. aPDT significantly reduced biofilm formation compared to the negative control (p < 0.001). The highest inhibitory effects were observed at 31.25 and 62.5 µg/mL, with effects comparable to those of 2% chlorhexidine under in vitro conditions. The highest concentration (2000 µg/mL) showed reduced activity and a potential paradoxical, concentration-dependent effect. Without light activation, the extract also showed antibiofilm activity, but to a lesser extent. Conclusion: Curcuma longa L. exhibits concentration-dependent antibiofilm activity against E. faecalis, enhanced by aPDT. Reduced efficacy at higher concentrations may be related to aggregation and self-quenching of curcumin. Despite chlorhexidine remaining the clinical reference, C. longa showed comparable effects at optimal concentrations. Limitations include the absence of a light-only control, lack of CFU confirmation, and potential staining risk. (Copyright © 2026 The Authors. Published by Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of competing interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Antimicrobial Photodynamic Therapy; Biofilms; Curcumin; Enterococcus faecalis; Photodynamic Therapy; Plant Extracts |
| Substance Nomenclature: | 0 (Plant Extracts) 0 (Photosensitizing Agents) 856YO1Z64F (turmeric extract) R4KO0DY52L (Chlorhexidine) |
| Entry Date(s): | Date Created: 20260516 Date Completed: 20260613 Latest Revision: 20260613 |
| Update Code: | 20260615 |
| DOI: | 10.1016/j.pdpdt.2026.105515 |
| PMID: | 42142792 |
| Βάση Δεδομένων: | MEDLINE |
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