Academic Journal
Combining fluorescence anisotropy and parallel factor analysis to estimate the degree of polymerization in red wine.
| Τίτλος: | Combining fluorescence anisotropy and parallel factor analysis to estimate the degree of polymerization in red wine. |
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| Συγγραφείς: | Halberg HFS; Department of Food Science, University of Copenhagen, Copenhagen, Denmark; FOSS Analytical A/S, Hillerød, Denmark., Heide ST; FOSS Analytical A/S, Hillerød, Denmark., Rinnan ÅS; Department of Food Science, University of Copenhagen, Copenhagen, Denmark. Electronic address: aar@food.ku.dk. |
| Πηγή: | Food research international (Ottawa, Ont.) [Food Res Int] 2026 Jul 31; Vol. 236, pp. 119152. Date of Electronic Publication: 2026 Apr 24. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science Country of Publication: Canada NLM ID: 9210143 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7145 (Electronic) Linking ISSN: 09639969 NLM ISO Abbreviation: Food Res Int Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Ottawa, Ontario, Canada : Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science, c1992- |
| Ιατρικοί όροι (MeSH): | Wine*/analysis , Fluorescence Polarization*/methods , Tannins*/analysis , Tannins*/chemistry , Polymerization*, Catechin/analysis ; Factor Analysis, Statistical ; Spectrometry, Fluorescence |
| Περίληψη: | Tannins in red wine are polymer structures of flavanols, such as catechin and epicatechin, and their degree of polymerization affects sensory attributes, especially bitterness and astringency. Traditional analytical methods for assessing the degree of polymerization are tedious and involve extensive sample preparation. In this study, we have investigated fluorescence spectroscopy with and without polarization as a faster alternative. Two sample sets were used to study this: a simple sample system consisting of six chemical standards and a set of six red wine samples. The samples were diluted prior to fluorescence excitation-emission matrix measurements, and the three-way data were decomposed using the trilinear model Parallel Factor Analysis (PARAFAC). With the non-polarized measurements we show that when flavanols polymerize the excitation/emission position of the fluorescent signal does not change, but the quantum yield decreases. For the polarization measurements (anisotropy), the PARAFAC model succeeded in resolving the flavanol fluorescent component, which was used to calculate the polarization of the samples. The polarization results showed a strong correlation (r2 = 0.94) with the traditional reference method for estimating the degree of polymerization. Our approach combines fluorescence anisotropy with PARAFAC decomposition for calculating polarization. This is a promising novel method for estimating the mean degree of polymerization, both in simple sample systems and in real red wine samples. (Copyright © 2026 The Authors. Published by Elsevier Ltd.. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Helene Froriep Stengade Halberg reports financial support was provided by Innovation Fund Denmark (grant no. 2040-00031B). If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Anisotropy; Fluorescence spectroscopy; Parallel factor analysis (PARAFAC); Polymerization; Polyphenols; Red wine |
| Substance Nomenclature: | 8R1V1STN48 (Catechin) 0 (Tannins) |
| Entry Date(s): | Date Created: 20260512 Date Completed: 20260716 Latest Revision: 20260716 |
| Update Code: | 20260717 |
| DOI: | 10.1016/j.foodres.2026.119152 |
| PMID: | 42116449 |
| Βάση Δεδομένων: | MEDLINE |
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