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Stage-resolved transcriptomic analysis of GPCR expression during settlement and metamorphosis in the sea urchin Heliocidaris crassispina.

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Τίτλος: Stage-resolved transcriptomic analysis of GPCR expression during settlement and metamorphosis in the sea urchin Heliocidaris crassispina.
Συγγραφείς: Jia J; National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang, 316022, People's Republic of China., Ge L; National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang, 316022, People's Republic of China., Ding K; National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang, 316022, People's Republic of China., Yang J; National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang, 316022, People's Republic of China., Wang J; National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang, 316022, People's Republic of China., Weng J; National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang, 316022, People's Republic of China; Shengsi Haichuang Science and Technology Development Research Institute, Zhoushan, Zhejiang, 202452, People's Republic of China., Yang J; National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang, 316022, People's Republic of China. Electronic address: yangjingwen@zjou.edu.cn., Wang T; National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang, 316022, People's Republic of China. Electronic address: wangtianming@zjou.edu.cn.
Πηγή: Comparative biochemistry and physiology. Part D, Genomics & proteomics [Comp Biochem Physiol Part D Genomics Proteomics] 2026 Sep; Vol. 59, pp. 101853. Date of Electronic Publication: 2026 May 04.
Τύπος έκδοσης: Journal Article; Research Support, Non-U.S. Gov't
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101270611 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-0407 (Electronic) Linking ISSN: 1744117X NLM ISO Abbreviation: Comp Biochem Physiol Part D Genomics Proteomics Subsets: MEDLINE
Imprint Name(s): Original Publication: Amsterdam : Elsevier, 2006-
Ιατρικοί όροι (MeSH): Metamorphosis, Biological*/genetics , Receptors, G-Protein-Coupled*/genetics , Anthocidaris*/genetics , Anthocidaris*/growth & development , Transcriptome* , Gene Expression Regulation, Developmental*, Larva/genetics ; Larva/growth & development ; Animals ; Gene Expression Profiling
Περίληψη: Larval settlement and metamorphosis are critical developmental transitions in sea urchins and represent a major bottleneck in aquaculture. Here, we used stage-resolved transcriptome sequencing to characterize genome-wide gene expression dynamics during settlement and metamorphosis in the purple sea urchin Heliocidaris crassispina, with a particular focus on G protein-coupled receptors (GPCRs). Based on a refined chromosome-level genome annotation, 763 GPCR candidate genes were identified and their expression patterns were systematically examined across six-arm larvae, eight-arm competent larvae, and early juveniles. Transcriptomic analyses revealed pronounced stage-dependent remodeling, with the competent larval stage showing a marked expansion of expressed GPCRs. Time-series clustering identified two major GPCR expression patterns: one associated with the competence stage and characterized by transient or peak-shaped upregulation, and another that became more prominent after settlement and remained highly expressed in early juveniles. PPI network analysis further suggested that these temporal patterns were associated with coordinated network organization rather than isolated receptor-level changes. Representative GPCR expression patterns were independently validated by qRT-PCR. These results provide a stage-resolved transcriptomic framework for understanding GPCR expression dynamics during sea urchin settlement and metamorphosis and offer a basis for future functional studies in echinoderms.
(Copyright © 2026. Published by Elsevier Inc.)
Competing Interests: Declaration of competing interest The authors declare that they have no competing financial interests.
Contributed Indexing: Keywords: G protein-coupled receptors; Heliocidaris crassispina; Metamorphosis; RNA-Seq; Temporal gene expression
Molecular Sequence: BioProject PRJNA1426057
Substance Nomenclature: 0 (Receptors, G-Protein-Coupled)
Entry Date(s): Date Created: 20260509 Date Completed: 20260606 Latest Revision: 20260610
Update Code: 20260610
DOI: 10.1016/j.cbd.2026.101853
PMID: 42105464
Βάση Δεδομένων: MEDLINE
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  Data: Stage-resolved transcriptomic analysis of GPCR expression during settlement and metamorphosis in the sea urchin Heliocidaris crassispina.
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  Data: <searchLink fieldCode="AU" term="%22Jia+J%22">Jia J</searchLink>; National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang, 316022, People's Republic of China.<br /><searchLink fieldCode="AU" term="%22Ge+L%22">Ge L</searchLink>; National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang, 316022, People's Republic of China.<br /><searchLink fieldCode="AU" term="%22Ding+K%22">Ding K</searchLink>; National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang, 316022, People's Republic of China.<br /><searchLink fieldCode="AU" term="%22Yang+J%22">Yang J</searchLink>; National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang, 316022, People's Republic of China.<br /><searchLink fieldCode="AU" term="%22Wang+J%22">Wang J</searchLink>; National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang, 316022, People's Republic of China.<br /><searchLink fieldCode="AU" term="%22Weng+J%22">Weng J</searchLink>; National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang, 316022, People's Republic of China; Shengsi Haichuang Science and Technology Development Research Institute, Zhoushan, Zhejiang, 202452, People's Republic of China.<br /><searchLink fieldCode="AU" term="%22Yang+J%22">Yang J</searchLink>; National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang, 316022, People's Republic of China. Electronic address: yangjingwen@zjou.edu.cn.<br /><searchLink fieldCode="AU" term="%22Wang+T%22">Wang T</searchLink>; National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang, 316022, People's Republic of China. Electronic address: wangtianming@zjou.edu.cn.
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  Data: Larval settlement and metamorphosis are critical developmental transitions in sea urchins and represent a major bottleneck in aquaculture. Here, we used stage-resolved transcriptome sequencing to characterize genome-wide gene expression dynamics during settlement and metamorphosis in the purple sea urchin Heliocidaris crassispina, with a particular focus on G protein-coupled receptors (GPCRs). Based on a refined chromosome-level genome annotation, 763 GPCR candidate genes were identified and their expression patterns were systematically examined across six-arm larvae, eight-arm competent larvae, and early juveniles. Transcriptomic analyses revealed pronounced stage-dependent remodeling, with the competent larval stage showing a marked expansion of expressed GPCRs. Time-series clustering identified two major GPCR expression patterns: one associated with the competence stage and characterized by transient or peak-shaped upregulation, and another that became more prominent after settlement and remained highly expressed in early juveniles. PPI network analysis further suggested that these temporal patterns were associated with coordinated network organization rather than isolated receptor-level changes. Representative GPCR expression patterns were independently validated by qRT-PCR. These results provide a stage-resolved transcriptomic framework for understanding GPCR expression dynamics during sea urchin settlement and metamorphosis and offer a basis for future functional studies in echinoderms.<br /> (Copyright © 2026. Published by Elsevier Inc.)
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  Data: Declaration of competing interest The authors declare that they have no competing financial interests.
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        StartPage: 101853
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      – SubjectFull: Larva genetics
        Type: general
      – SubjectFull: Larva growth & development
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      – SubjectFull: Animals
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