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Partial Denitrification-Mediated anammox Evolution in anoxic Compartments: Deciphering metabolic activity and microbial community.

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Τίτλος: Partial Denitrification-Mediated anammox Evolution in anoxic Compartments: Deciphering metabolic activity and microbial community.
Συγγραφείς: Li J; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China., Wu Y; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China., Li X; Sinopec Beijing Research Institute of Chemical Industry, Beijing 100013, China., Gao R; Power China Huadong Engineering Corporation Limited, Hangzhou 311122, China., Chen X; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China., Zhang S; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China., Zhang J; Beijing Tansi Environmental Protection Technology Co., Ltd, Beijing 100081, China., Zhang L; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China., Zhang S; Beijing Drainage Group Co. Ltd, Beijing 100022, China., Peng Y; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China. Electronic address: pyz@bjut.edu.cn.
Πηγή: Bioresource technology [Bioresour Technol] 2026 Sep; Vol. 455, pp. 134812. Date of Electronic Publication: 2026 May 06.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Elsevier Applied Science Country of Publication: England NLM ID: 9889523 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2976 (Electronic) Linking ISSN: 09608524 NLM ISO Abbreviation: Bioresour Technol Subsets: MEDLINE
Imprint Name(s): Original Publication: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991-
Ιατρικοί όροι (MeSH): Ammonium Compounds*/metabolism , Denitrification* , Anaerobic Ammonia Oxidation*, Bioreactors/microbiology ; Nitrogen/isolation & purification ; Nitrogen/metabolism ; Wastewater/chemistry ; Bacteria/metabolism ; Bacteria/genetics ; Biofilms ; Anaerobiosis ; Oxidation-Reduction
Περίληψη: The integration of partial denitrification (PD, NO3-→NO2-) with anaerobic ammonium oxidation (Anammox) in anoxic biofilm systems presents a transformative approach for enhanced nitrogen removal from municipal wastewater. Through a 7-month comparative analysis of spatially stratified anoxic zones in an anaerobic-anoxic-oxic bioreactor treating real wastewater (NH4+: 47.6 ± 4.7 mg N/L; COD: 154.8 ± 29.6 mg/L), this study achieved 71.8 ± 5.8% total nitrogen removal (effluent TN: 12.9 ± 3.9 mg N/L), aiming to propose optimization frameworks targeting biofilm carrier deployment in the anoxic zone. Test results showed that functional dominance partitioning emerged as a key determinant: the first anoxic zone (A1) exhibited peak anammox activity (0.034 kg N/m3/d) via rapid acetate-driven nitrate reduction, while the third zone (A3) sustained maximum Ca. Brocadia abundance (1.7%). Metagenomic sequencing further revealed that the highest ratio of NO3- reductase gene (narG) to NO2- reductase genes (nirS, nirK) was 2.06 in A3 compared to 1.39-1.68 in the other biofilms, indicating a stronger ability to supply NO2- to anammox. Carbon metabolic gene distribution revealed A1's acetate/glucose preference versus A3's endogenous metabolism dominance (elevated TCA cycle genes). This study proposes an innovative biofilm management framework for energy-efficient municipal wastewater treatment: front-positioned carriers maximize anammox nitrogen removal under moderate carbon-to-nitrogen ratios (3-5), while rear-positioned units secure anammox biomass retention during carbon surges.
(Copyright © 2026 Elsevier Ltd. All rights reserved.)
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Contributed Indexing: Keywords: Anoxic zone; Biofilms; Metagenomic sequencing; Municipal wastewater; Nitrogen removal
Substance Nomenclature: N762921K75 (Nitrogen)
0 (Wastewater)
0 (Ammonium Compounds)
Entry Date(s): Date Created: 20260508 Date Completed: 20260716 Latest Revision: 20260716
Update Code: 20260716
DOI: 10.1016/j.biortech.2026.134812
PMID: 42102935
Βάση Δεδομένων: MEDLINE
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  Data: Partial Denitrification-Mediated anammox Evolution in anoxic Compartments: Deciphering metabolic activity and microbial community.
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  Data: <searchLink fieldCode="AU" term="%22Li+J%22">Li J</searchLink>; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China.<br /><searchLink fieldCode="AU" term="%22Wu+Y%22">Wu Y</searchLink>; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China.<br /><searchLink fieldCode="AU" term="%22Li+X%22">Li X</searchLink>; Sinopec Beijing Research Institute of Chemical Industry, Beijing 100013, China.<br /><searchLink fieldCode="AU" term="%22Gao+R%22">Gao R</searchLink>; Power China Huadong Engineering Corporation Limited, Hangzhou 311122, China.<br /><searchLink fieldCode="AU" term="%22Chen+X%22">Chen X</searchLink>; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China.<br /><searchLink fieldCode="AU" term="%22Zhang+S%22">Zhang S</searchLink>; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China.<br /><searchLink fieldCode="AU" term="%22Zhang+J%22">Zhang J</searchLink>; Beijing Tansi Environmental Protection Technology Co., Ltd, Beijing 100081, China.<br /><searchLink fieldCode="AU" term="%22Zhang+L%22">Zhang L</searchLink>; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China.<br /><searchLink fieldCode="AU" term="%22Zhang+S%22">Zhang S</searchLink>; Beijing Drainage Group Co. Ltd, Beijing 100022, China.<br /><searchLink fieldCode="AU" term="%22Peng+Y%22">Peng Y</searchLink>; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China. Electronic address: pyz@bjut.edu.cn.
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  Data: <searchLink fieldCode="MM" term="%22Ammonium+Compounds%22">Ammonium Compounds*</searchLink>/<searchLink fieldCode="MM" term="%22Ammonium+Compounds+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Denitrification%22">Denitrification*</searchLink> <br /><searchLink fieldCode="MM" term="%22Anaerobic+Ammonia+Oxidation%22">Anaerobic Ammonia Oxidation*</searchLink><br /><searchLink fieldCode="MH" term="%22Bioreactors%22">Bioreactors</searchLink>/<searchLink fieldCode="MH" term="%22Bioreactors+microbiology%22">microbiology</searchLink> ; <searchLink fieldCode="MH" term="%22Nitrogen%22">Nitrogen</searchLink>/<searchLink fieldCode="MH" term="%22Nitrogen+isolation+%26+purification%22">isolation & purification</searchLink> ; <searchLink fieldCode="MH" term="%22Nitrogen%22">Nitrogen</searchLink>/<searchLink fieldCode="MH" term="%22Nitrogen+metabolism%22">metabolism</searchLink> ; <searchLink fieldCode="MH" term="%22Wastewater%22">Wastewater</searchLink>/<searchLink fieldCode="MH" term="%22Wastewater+chemistry%22">chemistry</searchLink> ; <searchLink fieldCode="MH" term="%22Bacteria%22">Bacteria</searchLink>/<searchLink fieldCode="MH" term="%22Bacteria+metabolism%22">metabolism</searchLink> ; <searchLink fieldCode="MH" term="%22Bacteria%22">Bacteria</searchLink>/<searchLink fieldCode="MH" term="%22Bacteria+genetics%22">genetics</searchLink> ; <searchLink fieldCode="MH" term="%22Biofilms%22">Biofilms</searchLink> ; <searchLink fieldCode="MH" term="%22Anaerobiosis%22">Anaerobiosis</searchLink> ; <searchLink fieldCode="MH" term="%22Oxidation-Reduction%22">Oxidation-Reduction</searchLink>
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  Data: The integration of partial denitrification (PD, NO<subscript>3</subscript><superscript>-</superscript>→NO<subscript>2</subscript><superscript>-</superscript>) with anaerobic ammonium oxidation (Anammox) in anoxic biofilm systems presents a transformative approach for enhanced nitrogen removal from municipal wastewater. Through a 7-month comparative analysis of spatially stratified anoxic zones in an anaerobic-anoxic-oxic bioreactor treating real wastewater (NH<subscript>4</subscript><superscript>+</superscript>: 47.6 ± 4.7 mg N/L; COD: 154.8 ± 29.6 mg/L), this study achieved 71.8 ± 5.8% total nitrogen removal (effluent TN: 12.9 ± 3.9 mg N/L), aiming to propose optimization frameworks targeting biofilm carrier deployment in the anoxic zone. Test results showed that functional dominance partitioning emerged as a key determinant: the first anoxic zone (A1) exhibited peak anammox activity (0.034 kg N/m<superscript>3</superscript>/d) via rapid acetate-driven nitrate reduction, while the third zone (A3) sustained maximum Ca. Brocadia abundance (1.7%). Metagenomic sequencing further revealed that the highest ratio of NO<subscript>3</subscript><superscript>-</superscript> reductase gene (narG) to NO<subscript>2</subscript><superscript>-</superscript> reductase genes (nirS, nirK) was 2.06 in A3 compared to 1.39-1.68 in the other biofilms, indicating a stronger ability to supply NO<subscript>2</subscript><superscript>-</superscript> to anammox. Carbon metabolic gene distribution revealed A1's acetate/glucose preference versus A3's endogenous metabolism dominance (elevated TCA cycle genes). This study proposes an innovative biofilm management framework for energy-efficient municipal wastewater treatment: front-positioned carriers maximize anammox nitrogen removal under moderate carbon-to-nitrogen ratios (3-5), while rear-positioned units secure anammox biomass retention during carbon surges.<br /> (Copyright © 2026 Elsevier Ltd. All rights reserved.)
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  Data: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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  Data: <i>Keywords: </i>Anoxic zone; Biofilms; Metagenomic sequencing; Municipal wastewater; Nitrogen removal
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