Doxycycline-induced toxic perturbations on manure GHG potential: Phage-microbe interactions and BSF pretreatment mitigation.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	Doxycycline-induced toxic perturbations on manure GHG potential: Phage-microbe interactions and BSF pretreatment mitigation.
Συγγραφείς:	Deng WK; College of Animal Science, South China Agricultural University, Guangzhou, Guangdong 510642, China., Deng YH; College of Animal Science, South China Agricultural University, Guangzhou, Guangdong 510642, China., Xie SY; College of Animal Science, South China Agricultural University, Guangzhou, Guangdong 510642, China., Chen JY; College of Animal Science, South China Agricultural University, Guangzhou, Guangdong 510642, China., Xing SC; Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou 510642, China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry Agriculture, Guangzhou, Guangdong 510642, China; National-Local Joint Engineering Research Center for Livestock Breeding, Guangzhou, Guangdong 510642, China., Liao XD; College of Animal Science, South China Agricultural University, Guangzhou, Guangdong 510642, China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry Agriculture, Guangzhou, Guangdong 510642, China; National-Local Joint Engineering Research Center for Livestock Breeding, Guangzhou, Guangdong 510642, China; State Key Laboratory of Swine and Poultry Breeding Industry, Guangzhou, Guangdong 510642, China. Electronic address: xdliao@scau.edu.cn.
Πηγή:	Ecotoxicology and environmental safety [Ecotoxicol Environ Saf] 2026 Jun 15; Vol. 318, pp. 120204. Date of Electronic Publication: 2026 May 07.
Τύπος έκδοσης:	Journal Article
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7805381 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2414 (Electronic) Linking ISSN: 01476513 NLM ISO Abbreviation: Ecotoxicol Environ Saf Subsets: MEDLINE
Imprint Name(s):	Original Publication: Amsterdam, Netherlands : Elsevier
Ιατρικοί όροι (MeSH):	Manure/microbiology , Manure/analysis , Doxycycline/toxicity , Bacteriophages/physiology , Bacteriophages/drug effects , Anti-Bacterial Agents/toxicity , Greenhouse Gases/analysis , Greenhouse Gases/metabolism , Air Pollutants/analysis , Air Pollutants/metabolism, Methane/metabolism ; Methane/analysis ; Nitrous Oxide/metabolism ; Nitrous Oxide/analysis ; Feces/chemistry ; Feces/microbiology ; Animals ; Chickens
Περίληψη:	The large-scale production of poultry manure poses significant environmental safety challenges, and Black Soldier Fly (BSF) has emerged as a potential resource utilization solution. However, the toxicological effects of doxycycline (DOX) contamination in poultry feces on methane (CH4) and nitrous oxide (N2O) emission potential throughout the BSF manure recycling process remains unclear, especially from a phage-microbe interaction perspective. This study investigates the roles of bacterial and phage-related functional genes associated with CH4 and N2O metabolism in the "chicken manure-organic fertilizer" and "chicken manure-BSF-chicken" pathways. The results reveal that DOX in poultry manure causes toxicological perturbations of the microbial community, significantly elevating the levels of key functional genes (mcrA/pmoA and (nirS+nirK)/nosZ) linked to GHG emission potential. These DOX-induced elevated gene levels persisted in BSF-derived organic fertilizer and in the feces of laying hens fed BSF reared on contaminated manure. However, 24-hour starvation pretreatment combined with 8-hour drying at 65 °C can effectively alleviate the negative toxicological effects induced by DOX, and the emission potentials of CH₄ and N₂O in the feces of laying hens fed BSF treated in this way were reduced by 91.1% and 82.4%, respectively. Importantly, phage-mediated horizontal gene transfer (HGT) plays a significant regulatory role in these gene changes, further amplifying the DOX-induced GHG emission risk. This study highlights the potential of BSF pretreatment to reduce the environmental risk of antibiotic-contaminated poultry manure while addressing climate concerns from an environmental toxicology perspective. It also provides a scalable toxicological risk mitigation strategy to reduce greenhouse gas emission potential in poultry farming systems, which is of great significance for the environmental safety of intensive poultry production. (Copyright © 2026 The Authors. Published by Elsevier Inc. All rights reserved.)
Competing Interests:	Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Contributed Indexing:	Keywords: Doxycycline; Environmental toxicology; Greenhouse gas emission potential; Phage-microbe interaction; Poultry manure
Substance Nomenclature:	0 (Manure) N12000U13O (Doxycycline) OP0UW79H66 (Methane) 0 (Anti-Bacterial Agents) K50XQU1029 (Nitrous Oxide) 0 (Greenhouse Gases) 0 (Air Pollutants)
Entry Date(s):	Date Created: 20260508 Date Completed: 20260716 Latest Revision: 20260716
Update Code:	20260716
DOI:	10.1016/j.ecoenv.2026.120204
PMID:	42102708
Βάση Δεδομένων:	MEDLINE

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