Academic Journal
Neutralizing epitope mapping for deltacoronavirus receptor-binding domain and seroepidemiological survey across different animal species.
| Τίτλος: | Neutralizing epitope mapping for deltacoronavirus receptor-binding domain and seroepidemiological survey across different animal species. |
|---|---|
| Συγγραφείς: | Li D; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, China., Hao L; Luoyang Putai Biotechnology Co. Ltd, Luoyang, China., Huang B; Zhejiang Lab, Hangzhou, Zhejiang, China., Wang Z; Luoyang Putai Biotechnology Co. Ltd, Luoyang, China., Wang Z; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, China., Guo M; Zhejiang Lab, Hangzhou, Zhejiang, China.; School of Life Sciences and Technology, Tongji University, Shanghai, China., Zhang Y; National Research Center for Veterinary Medicine, Luoyang, China., Deng J; Luoyang Putai Biotechnology Co. Ltd, Luoyang, China., Li Y; Luoyang Putai Biotechnology Co. Ltd, Luoyang, China., Zhao S; Luoyang Putai Biotechnology Co. Ltd, Luoyang, China., Tian K; National Research Center for Veterinary Medicine, Luoyang, China., Li X; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, China.; Jiangsu Key Laboratory of Zoonosis, Jiangsu Interdisciplinary Center for Zoonoses and Biosafety, Yangzhou University, Yangzhou, PR China. |
| Πηγή: | Virulence [Virulence] 2026 Dec 31; Vol. 17 (1), pp. 2668161. Date of Electronic Publication: 2026 May 07. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101531386 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2150-5608 (Electronic) Linking ISSN: 21505594 NLM ISO Abbreviation: Virulence Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2015- : Philadelphia, PA : Taylor & Francis Original Publication: Austin, Tex. : Landes Bioscience |
| Ιατρικοί όροι (MeSH): | Deltacoronavirus*/immunology , Deltacoronavirus*/genetics , Antibodies, Neutralizing*/immunology , Swine Diseases*/virology , Swine Diseases*/epidemiology , Swine Diseases*/immunology , Coronavirus Infections*/veterinary , Coronavirus Infections*/epidemiology , Coronavirus Infections*/virology , Coronavirus Infections*/immunology , Receptors, Virus*/immunology , Receptors, Virus*/metabolism , Epitope Mapping*, Antibodies, Monoclonal/immunology ; Antibodies, Viral/immunology ; Antibodies, Viral/blood ; Epitopes/immunology ; Enzyme-Linked Immunosorbent Assay/methods ; Animals ; Swine ; Seroepidemiologic Studies ; Neutralization Tests |
| Περίληψη: | Porcine deltacoronavirus (PDCoV) is an emerging enteric pathogen that poses a significant threat to the global swine industry and carries a potential public health risk. The development of neutralizing monoclonal antibodies (mAbs) is crucial for preventing and controlling PDCoV. However, research on neutralizing linear epitopes within the PDCoV receptor-binding domain (RBD) and the establishment of serological assays based on such mAbs remains limited. In this study, we generated two neutralizing mAbs, 1C12 and 3A11, targeting the PDCoV RBD. Epitope mapping revealed that 1C12 recognizes a linear epitope (316DFGEARLD323), whereas 3A11 binds to a conformational epitope. Further residue analysis identified Arg321 as the most critical residue for binding to 1C12. Additionally, the integrity of the entire 321RLD323 motif was found to be indispensable for the conformational recognition by mAb 3A11. Using these mAbs, we developed two blocking enzyme-linked immunosorbent assay (bELISA) methods (bELISA-1C12 and bELISA-3A11). Evaluation of 150 clinical samples demonstrated that bELISA-1C12 exhibited 93.3% concordance with a virus neutralization test (VNT), indicating higher diagnostic sensitivity than bELISA-3A11 (92.7%). A large-scale serosurvey of pig populations across ten Chinese provinces in 2025, conducted using bELISA-1C12, revealed a PDCoV seropositivity rate of 13.6%. Notably, when extending the serological screening to 21 additional animal species, we detected PDCoV antibodies in peafowl serum for the first time, suggesting possible exposure to the virus. This study identifies key antigenic epitopes on the PDCoV RBD and provides valuable tools for epidemiological surveillance and assessing the transmission risk of PDCoV. |
| References: | BMC Vet Res. 2016 Jun 08;12:95. (PMID: 27277214) Sci China Life Sci. 2025 Dec;68(12):3756-3774. (PMID: 41144156) Arch Virol. 2020 Apr;165(4):845-851. (PMID: 32052195) Nat Commun. 2024 Jun 22;15(1):5330. (PMID: 38909062) J Virol. 2025 Feb 25;99(2):e0202524. (PMID: 39840987) Transbound Emerg Dis. 2017 Feb;64(1):3-10. (PMID: 27718337) Science. 2020 Aug 21;369(6506):1014-1018. (PMID: 32540904) Nucleic Acids Res. 2017 Jul 3;45(W1):W24-W29. (PMID: 28472356) Transbound Emerg Dis. 2025 Nov 24;2025:3988285. (PMID: 41333610) Int J Mol Sci. 2023 Dec 04;24(23):. (PMID: 38069424) PLoS Pathog. 2025 Jan 07;21(1):e1012836. (PMID: 39774464) Virulence. 2026 Dec;17(1):2614154. (PMID: 41503690) Adv Sci (Weinh). 2025 Dec;12(46):e11920. (PMID: 40985348) Emerg Microbes Infect. 2024 Dec;13(1):2400559. (PMID: 39222358) Epidemiology. 2005 Jan;16(1):73-81. (PMID: 15613948) J Vet Med Sci. 2016 May 3;78(4):601-6. (PMID: 26668175) Virus Res. 2024 Feb;340:199303. (PMID: 38145807) Cancer. 1950 Jan;3(1):32-5. (PMID: 15405679) J Virol. 2012 Apr;86(7):3995-4008. (PMID: 22278237) N Engl J Med. 2020 Feb 20;382(8):727-733. (PMID: 31978945) Infect Genet Evol. 2018 Jul;61:176-182. (PMID: 29621617) Transbound Emerg Dis. 2017 Oct;64(5):1364-1370. (PMID: 28758347) Transbound Emerg Dis. 2025 Jun 4;2025:2339024. (PMID: 40503219) Virus Res. 2016 Dec 2;226:71-84. (PMID: 27270129) J Clin Microbiol. 2025 Mar 12;63(3):e0161524. (PMID: 39950715) J Virol. 2021 Nov 23;95(24):e0111821. (PMID: 34549985) Transbound Emerg Dis. 2019 Jul;66(4):1436-1441. (PMID: 30941894) Virulence. 2026 Dec 31;17(1):2668161. (PMID: 42093621) J Virol. 2018 Jul 17;92(15):. (PMID: 29769348) Int J Biol Macromol. 2023 Jul 1;242(Pt 4):125190. (PMID: 37276902) Nat Commun. 2022 Mar 18;13(1):1467. (PMID: 35304871) Sci Rep. 2024 Jan 30;14(1):2464. (PMID: 38291341) J Virol. 2024 Sep 17;98(9):e0053524. (PMID: 39158273) Proc Natl Acad Sci U S A. 2018 May 29;115(22):E5135-E5143. (PMID: 29760102) Transbound Emerg Dis. 2018 Oct;65(5):1197-1207. (PMID: 29607611) J Virol Methods. 2017 Nov;249:76-78. (PMID: 28860101) Viruses. 2022 Aug 18;14(8):. (PMID: 36016437) Emerg Infect Dis. 2014 Jul;20(7):1227-30. (PMID: 24964136) Trends Microbiol. 2016 Jun;24(6):490-502. (PMID: 27012512) J Virol. 2023 Nov 30;97(11):e0095823. (PMID: 37846983) Viruses. 2019 Jun 21;11(6):. (PMID: 31234434) Genome Announc. 2014 Nov 26;2(6):. (PMID: 25428966) PLoS One. 2015 Apr 16;10(4):e0124363. (PMID: 25881086) Clin Infect Dis. 2022 Feb 11;74(3):446-454. (PMID: 34013321) Nat Rev Mol Cell Biol. 2022 Jan;23(1):3-20. (PMID: 34611326) Nature. 2021 Dec;600(7887):133-137. (PMID: 34789872) J Gen Virol. 2017 Feb;98(2):173-178. (PMID: 27995863) Arch Virol. 2017 Aug;162(8):2357-2362. (PMID: 28374120) Immunity. 2024 Dec 10;57(12):2914-2927.e7. (PMID: 39488210) Cell. 2018 Mar 8;172(6):1157-1159. (PMID: 29522735) Transbound Emerg Dis. 2015 Dec;62(6):575-80. (PMID: 26250097) Int J Biol Macromol. 2024 Oct;278(Pt 4):135049. (PMID: 39182883) Transbound Emerg Dis. 2025 Jun 10;2025:9997711. (PMID: 40529623) Transbound Emerg Dis. 2022 Sep;69(5):e1561-e1573. (PMID: 35184388) J Virol Methods. 2023 Oct;320:114775. (PMID: 37482197) |
| Contributed Indexing: | Keywords: Porcine deltacoronavirus; blocking ELISA; monoclonal antibody; neutralizing epitope |
| Substance Nomenclature: | 0 (Antibodies, Monoclonal) 0 (Antibodies, Viral) 0 (Antibodies, Neutralizing) 0 (Epitopes) 0 (Receptors, Virus) |
| Entry Date(s): | Date Created: 20260507 Date Completed: 20260716 Latest Revision: 20260813 |
| Update Code: | 20260813 |
| PubMed Central ID: | PMC13166184 |
| DOI: | 10.1080/21505594.2026.2668161 |
| PMID: | 42093621 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 2150-5608 |
|---|---|
| DOI: | 10.1080/21505594.2026.2668161 |