Academic Journal
Only intercalating ions cause the temperature sensitivity in acridine orange-stained artificial nine-plasmid system-2.
| Τίτλος: | Only intercalating ions cause the temperature sensitivity in acridine orange-stained artificial nine-plasmid system-2. |
|---|---|
| Συγγραφείς: | Tang N; Department of Pathogen Biology, Chengdu Medical College, Chengdu, China., Peng C; General Practice Department, Second Affiliated Hospital, Chengdu Medical College, Chengdu, China., Liu F; Medical Records Department, First Affiliated Hospital, Chengdu Medical College, Chengdu, China., Liu T; Department of Pathogen Biology, Chengdu Medical College, Chengdu, China., Wu J; Department of General Surgery, Hanyuan County People's Hospital, Ya'an, China., Yang X; Department of General Surgery, Hanyuan County People's Hospital, Ya'an, China. 476211922@qq.com., Pan Q; Department of Pathogen Biology, Chengdu Medical College, Chengdu, China. ppqlove@126.com. |
| Πηγή: | Scientific reports [Sci Rep] 2026 May 06; Vol. 16 (1). Date of Electronic Publication: 2026 May 06. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: London : Nature Publishing Group, copyright 2011- |
| Ιατρικοί όροι (MeSH): | Acridine Orange*/chemistry , Plasmids*/chemistry , Intercalating Agents*/chemistry , Ions*/chemistry, DNA/chemistry ; Temperature ; Spectrometry, Fluorescence |
| Περίληψη: | This study aimed to investigate which ions influence the temperature sensitivity of acridine orange (AO)-stained nine-plasmid system-2. At 5 ℃ and 25 ℃, the nine-plasmid system-2 was electrophoresed on an undyed agarose gel, followed by post-staining with AO solutions containing different ions to detect their effects on the temperature sensitivity. Then, UV-Vis absorption and fluorescence spectra of the system combined with various ion-AO solutions were measured at 5 ℃-45 ℃, and ANOVA was used for intergroup comparisons. At 5 ℃, post-staining with the Tris-AO solution yielded 9, 4, 4, 2, and 1 bands, while post-staining with the AMPD-AO solution produced 9, 6, 4, 3, and 1 bands. Post-staining with other ion-AO solutions resulted in unchanged band numbers at 5 °C, and no temperature sensitivity was observed. Spectral measurements demonstrated that Tris-AO and AMPD-AO solutions containing the system exhibited significant fluctuations in optical signal intensity with temperature, showing temperature sensitivity. The temperature sensitivity correlates with the interference effect of specific ions on AO insertion into DNA. The organic weak base cations Tris and AMPD, which possess approximately tetrahedral symmetric structures containing amino and hydroxymethyl groups, induce temperature sensitivity, which strengthens with higher ionic strength. Other ions can only reduce the staining effect of AO. (© 2026. The Author(s).) |
| Competing Interests: | Declarations. Competing interests: The authors declare no competing interests. |
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| Grant Information: | 24LHWMZY1-02 the Clinical Scientific Research Fund of Chengdu Medical College - Hanyuan County People's Hospital; 24LHBBYY1 the Clinical Scientific Research Fund of Chengdu Medical College - Nuclear Industry 416 Hospital |
| Contributed Indexing: | Keywords: Acridine orange; Fluorescence spectrum; Ions; Nine plasmid systems-2; Temperature-sensitivity; Ultraviolet-visible spectrum |
| Substance Nomenclature: | F30N4O6XVV (Acridine Orange) 0 (Intercalating Agents) 0 (Ions) 9007-49-2 (DNA) |
| Entry Date(s): | Date Created: 20260506 Date Completed: 20260707 Latest Revision: 20260813 |
| Update Code: | 20260813 |
| PubMed Central ID: | PMC13338076 |
| DOI: | 10.1038/s41598-026-51846-8 |
| PMID: | 42091628 |
| Βάση Δεδομένων: | MEDLINE |
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