Design and fabrication of a platform for circulating tumor cells (CTCs) isolation and detection by MiRGD@NTA@Au-Fe3O4 nanoparticles.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	Design and fabrication of a platform for circulating tumor cells (CTCs) isolation and detection by MiRGD@NTA@Au-Fe3O4 nanoparticles.
Συγγραφείς:	Abdollahi Z; Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, P. O. Box: 14115-154, Iran., Borghei YS; Center for Bioscience & Technology, Institute for Convergence Science & Technology, Sharif University of Technology, Iran., Hosseinkhani S; Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, P. O. Box: 14115-154, Iran. Electronic address: saman_h@modares.ac.ir.
Πηγή:	Biosensors & bioelectronics [Biosens Bioelectron] 2026 Sep 01; Vol. 307, pp. 118754. Date of Electronic Publication: 2026 Apr 30.
Τύπος έκδοσης:	Journal Article
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: Elsevier Advanced Technology Country of Publication: England NLM ID: 9001289 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4235 (Electronic) Linking ISSN: 09565663 NLM ISO Abbreviation: Biosens Bioelectron Subsets: MEDLINE
Imprint Name(s):	Publication: Oxford : Elsevier Advanced Technology Original Publication: [Barking, Essex, England] : Elsevier Applied Science, 1989-
Ιατρικοί όροι (MeSH):	Biosensing Techniques/methods , Neoplastic Cells, Circulating/pathology , Cell Separation/methods , Magnetite Nanoparticles/chemistry , Oligopeptides*/chemistry, Gold/chemistry ; Colorimetry/methods ; Metal Nanoparticles/chemistry ; Nickel/chemistry ; Humans ; Limit of Detection ; MDA-MB-231 Cells
Περίληψη:	To enable simple and rapid detection of circulating tumor cells (CTCs), we developed a targeted colorimetric biosensor based on MiRGD-functionalized Fe3O4-PEI-Au nanoparticles conjugated with TGA and Ni⁺²-NTA (MiRGD@NTA@Au-Fe3O4 nanoparticles). The biosensor enables magnetic separation of target cells and visual signal generation through the peroxidase-mimicking activity of Au-Fe3O4 nanoparticles, using TMB as a substrate. Colorimetric signals were monitored at 650 nm and discerned by the naked eye. Spectral data and fluorescence microscopy confirmed the specific binding of MiRGD peptides to cancer cells, ensuring discrimination from non-cancerous cells. Under optimized conditions, the assay exhibited a linear response for MDA-MB-231 cells suspended in PBS in the range of 5-5000 cells/mL, with a limit of detection (LOD) 6 cell/mL. The biosensor also demonstrated robust performance in human blood samples, with LOD 8 cell/mL, confirming its applicability in complex biological environments. These results highlight MiRGD@NTA@Au-Fe3O4 nanoparticles as a promising platform for simple, rapid, and high-sensitivity detection of CTCs, offering potential for clinical diagnostics and personalized cancer monitoring. (Copyright © 2026 Elsevier B.V. All rights reserved.)
Competing Interests:	Declaration of competing interest There is no conflict of interest to declare.
Contributed Indexing:	Keywords: Cancer diagnostics; Circulating tumor cells (CTCs); Colorimetric biosensor; Magnetic-gold nanoparticles; Peroxidase-mimicking activity; Targeted peptide
Substance Nomenclature:	7440-57-5 (Gold) 0 (Magnetite Nanoparticles) 7OV03QG267 (Nickel) 0 (Oligopeptides)
Entry Date(s):	Date Created: 20260505 Date Completed: 20260716 Latest Revision: 20260716
Update Code:	20260716
DOI:	10.1016/j.bios.2026.118754
PMID:	42085915
Βάση Δεδομένων:	MEDLINE

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