Academic Journal
Visualizing nanoplastic-metal ion co-exposure stress with an NIR biothiol responsive fluorescent probe.
| Τίτλος: | Visualizing nanoplastic-metal ion co-exposure stress with an NIR biothiol responsive fluorescent probe. |
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| Συγγραφείς: | Wei S; Shandong Key Laboratory of Coastal Zone Environmental Processes and Ecological Security, Shandong Engineering Research Center for Coastal Zone Ecological & Environmental Monitoring Technologies and Equipment, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China. liangweizhang@yic.ac.cn.; University of Chinese Academy of Sciences, Beijing 100049, China., Zhang L; Shandong Key Laboratory of Coastal Zone Environmental Processes and Ecological Security, Shandong Engineering Research Center for Coastal Zone Ecological & Environmental Monitoring Technologies and Equipment, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China. liangweizhang@yic.ac.cn.; University of Chinese Academy of Sciences, Beijing 100049, China., Tan J; Shandong Key Laboratory of Coastal Zone Environmental Processes and Ecological Security, Shandong Engineering Research Center for Coastal Zone Ecological & Environmental Monitoring Technologies and Equipment, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China. liangweizhang@yic.ac.cn.; University of Chinese Academy of Sciences, Beijing 100049, China., Liu S; College of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, China., Arabi M; Shandong Key Laboratory of Coastal Zone Environmental Processes and Ecological Security, Shandong Engineering Research Center for Coastal Zone Ecological & Environmental Monitoring Technologies and Equipment, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China. liangweizhang@yic.ac.cn., Ostovan A; Shandong Key Laboratory of Coastal Zone Environmental Processes and Ecological Security, Shandong Engineering Research Center for Coastal Zone Ecological & Environmental Monitoring Technologies and Equipment, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China. liangweizhang@yic.ac.cn., Zhuang X; College of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, China., Chen L; Shandong Key Laboratory of Coastal Zone Environmental Processes and Ecological Security, Shandong Engineering Research Center for Coastal Zone Ecological & Environmental Monitoring Technologies and Equipment, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China. liangweizhang@yic.ac.cn.; Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao 266237, China.; University of Chinese Academy of Sciences, Beijing 100049, China. |
| Πηγή: | The Analyst [Analyst] 2026 Jun 02; Vol. 151 (11), pp. 3291-3297. Date of Electronic Publication: 2026 Jun 02. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 0372652 Publication Model: Electronic Cited Medium: Internet ISSN: 1364-5528 (Electronic) Linking ISSN: 00032654 NLM ISO Abbreviation: Analyst Subsets: MEDLINE |
| Imprint Name(s): | Publication: Cambridge : Royal Society of Chemistry Original Publication: London : Chemical Society |
| Ιατρικοί όροι (MeSH): | Fluorescent Dyes*/chemistry , Fluorescent Dyes*/toxicity , Fluorescent Dyes*/chemical synthesis , Sulfhydryl Compounds*/analysis , Sulfhydryl Compounds*/chemistry , Mercury*/chemistry , Mercury*/toxicity , Glutathione*/analysis, Oxidative Stress/drug effects ; Polymethyl Methacrylate/chemistry ; Polymethyl Methacrylate/toxicity ; Naphthalimides/chemistry ; Naphthalimides/toxicity ; Naphthalimides/radiation effects ; Naphthalimides/chemical synthesis ; Cysteine/analysis ; Zebrafish ; Animals ; Fluorescent Chemosensor Compounds ; Humans ; HeLa Cells ; Limit of Detection ; Infrared Rays |
| Περίληψη: | Visualizing the combined toxic effects of micro(nano)plastics and heavy metal ions remains challenging due to the lack of suitable in situ imaging tools, and current co-exposure models are often simplified by simple mixing rather than using pre-formed complexes. In this work, a near-infrared biothiol fluorescent probe was developed based on a naphthalimide scaffold with an α,β-unsaturated acetyl group as a recognition site. The probe exhibits high sensitivity and selectivity toward biothiols, and the detection limit was determined to be 0.47 μM for Cys, 0.37 μM for GSH, and 0.95 μM for Hcy, respectively. This enables the monitoring of biothiol fluctuations in living cells and zebrafish. Using pre-formed PMMA-Hg2+ complexes as co-exposure pollutant models, we investigated nanoplastic-metal ion complex induced oxidative stress in living cells and zebrafish. Imaging results revealed that PMMA-Hg2+ complex co-exposure induces distinct biothiol fluctuation patterns compared to single exposure of PMMA or Hg2+. The surface charge alterations of complexes and lysosomal function were found to be involved in the process. This study provides visual evidence for elucidating the combined toxicity mechanisms of nanoplastic-heavy metal complex co-exposure. |
| Substance Nomenclature: | 0 (Fluorescent Dyes) 0 (Fluorescent Chemosensor Compounds) 0 (Sulfhydryl Compounds) FXS1BY2PGL (Mercury) GAN16C9B8O (Glutathione) 9011-14-7 (Polymethyl Methacrylate) 0 (Naphthalimides) K848JZ4886 (Cysteine) |
| Entry Date(s): | Date Created: 20260505 Date Completed: 20260616 Latest Revision: 20260616 |
| Update Code: | 20260616 |
| DOI: | 10.1039/d6an00278a |
| PMID: | 42084039 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1364-5528 |
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| DOI: | 10.1039/d6an00278a |