Academic Journal
The binding mechanism of an iron-chelating peptide from duck plasma and insights into its absorption-enhancing effect in Caco-2 cells.
| Τίτλος: | The binding mechanism of an iron-chelating peptide from duck plasma and insights into its absorption-enhancing effect in Caco-2 cells. |
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| Συγγραφείς: | Peng J; Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, PR China; School of Life Sciences, Anhui Normal University, Wuhu, Anhui 241001, PR China., Luo C; Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, PR China; School of Life Sciences, Anhui Normal University, Wuhu, Anhui 241001, PR China., Guo H; Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, PR China; School of Life Sciences, Anhui Normal University, Wuhu, Anhui 241001, PR China., Li Z; Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, PR China; School of Life Sciences and Food Engineering, Huaiyin Institute of Technology, Huaian, Jiangsu 223003, PR China., Zhou Y; Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, PR China., Zou Y; Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, PR China., Yang B; Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, PR China., Cheng M; Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, PR China., Ma J; Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, PR China., Xu W; Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, PR China., Wang D; Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, PR China., Yang J; Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, PR China; School of Life Sciences, Anhui Normal University, Wuhu, Anhui 241001, PR China. Electronic address: 20210007@jaas.ac.cn. |
| Πηγή: | Food research international (Ottawa, Ont.) [Food Res Int] 2026 Jul 01; Vol. 235, pp. 119166. Date of Electronic Publication: 2026 Apr 15. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science Country of Publication: Canada NLM ID: 9210143 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7145 (Electronic) Linking ISSN: 09639969 NLM ISO Abbreviation: Food Res Int Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Ottawa, Ontario, Canada : Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science, c1992- |
| Ιατρικοί όροι (MeSH): | Ducks*/blood , Iron Chelating Agents*/chemistry , Iron Chelating Agents*/pharmacology , Iron Chelating Agents*/metabolism , Iron*/metabolism , Peptides*/chemistry , Peptides*/blood , Peptides*/pharmacology , Peptides*/metabolism , Intestinal Absorption*/drug effects, Ferritins/metabolism ; Humans ; Caco-2 Cells ; Animals ; Molecular Docking Simulation ; Bioactive Peptides, Dietary ; Biological Availability |
| Περίληψη: | This study aimed to elucidate the Fe2+ chelation mechanism of a duck plasma-derived peptide (RCLQ) and its iron bioavailability-enhancing potential, exploring a high-value strategy for duck blood by-product utilization. The RCLQ-Fe complex was prepared and characterized. Structural characterization, molecular docking and molecular dynamics simulation were employed to identify the specific chelation sites and binding mechanism. Stability under varying pH, temperature, and simulated gastrointestinal digestion was assessed. A Caco-2 monolayer model evaluated iron absorption efficacy via cellular ferritin levels and key iron metabolism-related gene/protein expression. Molecular simulations and spectral data revealed Fe2+ primarily binds to the amide oxygen of Leu and Gln within RCLQ. The RCLQ-Fe chelate demonstrates remarkable stability, outperforming conventional ferrous sulfate. In Caco-2 cells, ferritin levels in RCLQ-Fe-treated groups exceeded those in ferrous sulfate groups; the high-concentration RCLQ-Fe group showed 6.16-fold greater iron transport than controls at 120 min, via upregulating Dmt1, IRP1, Dcytb and Epn1. This study elucidated the Fe2+ binding mechanism of RCLQ and iron absorption-enhancing pathways of RCLQ-Fe in Caco-2 cells. It provides a theoretical basis for RCLQ-Fe as a promising high-efficiency iron fortifier and a novel strategy for high-value utilization of duck blood by-products. (Copyright © 2026 Elsevier Ltd. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Binding mechanism; Bioavailability; Caco-2 cell; Duck plasma peptide; Ferrous-peptide chelate (RCLQ-Fe); Iron absorption |
| Substance Nomenclature: | 0 (Iron Chelating Agents) E1UOL152H7 (Iron) 0 (Peptides) 9007-73-2 (Ferritins) 0 (Bioactive Peptides, Dietary) |
| Entry Date(s): | Date Created: 20260505 Date Completed: 20260716 Latest Revision: 20260716 |
| Update Code: | 20260716 |
| DOI: | 10.1016/j.foodres.2026.119166 |
| PMID: | 42083210 |
| Βάση Δεδομένων: | MEDLINE |
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