Academic Journal
Protocol for identifying cellular reprogramming minimal networks using combinatorial transcription factor screening.
| Τίτλος: | Protocol for identifying cellular reprogramming minimal networks using combinatorial transcription factor screening. |
|---|---|
| Συγγραφείς: | Altman AR; Molecular Medicine and Gene Therapy, Science for Life Laboratory, Lund Stem Cell Center, Lund University, BMC A12, 221 84 Lund, Sweden; Wallenberg Centre for Molecular Medicine, Lund University, Lund, Sweden., Pértiga-Cabral D; Molecular Medicine and Gene Therapy, Science for Life Laboratory, Lund Stem Cell Center, Lund University, BMC A12, 221 84 Lund, Sweden; Wallenberg Centre for Molecular Medicine, Lund University, Lund, Sweden; CNC - Centre for Neuroscience and Cell Biology, University of Coimbra, Largo Marquês Do Pombal, 3004-517 Coimbra, Portugal; CiBB - Centre for Innovative Biomedicine and Biotechnology, University of Coimbra, Coimbra, Portugal; Doctoral Programme in Experimental Biology and Biomedicine, University of Coimbra, Coimbra, Portugal., Pereira CF; Molecular Medicine and Gene Therapy, Science for Life Laboratory, Lund Stem Cell Center, Lund University, BMC A12, 221 84 Lund, Sweden; Wallenberg Centre for Molecular Medicine, Lund University, Lund, Sweden; CNC - Centre for Neuroscience and Cell Biology, University of Coimbra, Largo Marquês Do Pombal, 3004-517 Coimbra, Portugal; CiBB - Centre for Innovative Biomedicine and Biotechnology, University of Coimbra, Coimbra, Portugal; Asgard Therapeutics AB, Medicon Village, 223 81 Lund, Sweden., Kurochkin I; Molecular Medicine and Gene Therapy, Science for Life Laboratory, Lund Stem Cell Center, Lund University, BMC A12, 221 84 Lund, Sweden; Wallenberg Centre for Molecular Medicine, Lund University, Lund, Sweden. Electronic address: ilia.kurochkin@med.lu.se. |
| Πηγή: | STAR protocols [STAR Protoc] 2026 Jun 19; Vol. 7 (2), pp. 104527. Date of Electronic Publication: 2026 Apr 28. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: [Cambridge, MA] : Cell Press, [2020]- |
| Ιατρικοί όροι (MeSH): | Transcription Factors*/metabolism , Transcription Factors*/genetics , Cellular Reprogramming*/genetics, Fibroblasts/cytology ; Fibroblasts/metabolism ; Lentivirus/genetics ; Humans |
| Περίληψη: | Direct reprogramming offers a powerful approach to generate therapeutic cell types, but progress is limited by an incomplete understanding of transcription factor (TF) cooperativity. Here, we present a protocol for performing combinatorial TF screening to resolve reprogramming factor networks that drive cell identity. We describe steps for arrayed lentiviral production, transduction, and reprogramming of human fibroblasts into distinct immune cells. We detail procedures for cell purification, library preparation, sequencing, and analysis to resolve TF combinations and dynamics. For complete details on the use and execution of this protocol, please refer to Kurochkin et al.1. (Copyright © 2026 The Authors. Published by Elsevier Inc. All rights reserved.) |
| Competing Interests: | Declaration of interests C.-F.P. has equity interests and serves in a management position at Asgard Therapeutics AB, which develops cancer immunotherapies based on DC reprogramming technologies. C.-F.P. is an inventor on granted patents U.S. 11,345,891, JP 7303743, and CN ZL201880005047.3 and on patent application WO2018/185709 held by Asgard Therapeutics that cover the cell reprogramming approach described here. |
| Contributed Indexing: | Keywords: Bioinformatics; Flow Cytometry; Immunology; RNAseq; Sequencing; Single Cell; Stem Cells |
| Substance Nomenclature: | 0 (Transcription Factors) |
| Entry Date(s): | Date Created: 20260429 Date Completed: 20260623 Latest Revision: 20260623 |
| Update Code: | 20260623 |
| PubMed Central ID: | PMC13138051 |
| DOI: | 10.1016/j.xpro.2026.104527 |
| PMID: | 42054212 |
| Βάση Δεδομένων: | MEDLINE |
καταχωρήστε σχόλιο πρώτοι!