Academic Journal
Integrating aptamer-transduced signal amplification and superwettable droplet microarrays for single-cell detection of cell surface vimentin.
| Τίτλος: | Integrating aptamer-transduced signal amplification and superwettable droplet microarrays for single-cell detection of cell surface vimentin. |
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| Συγγραφείς: | Miao X; Institute of Functional Nano and Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu Province, 215123, PR China., Lang G; Institute of Functional Nano and Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu Province, 215123, PR China., Xiao X; Institute of Functional Nano and Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu Province, 215123, PR China., Yang S; Institute of Functional Nano and Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu Province, 215123, PR China., Zhao J; Institute of Functional Nano and Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu Province, 215123, PR China., Zhou M; Institute of Functional Nano and Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu Province, 215123, PR China., Wang X; Institute of Functional Nano and Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu Province, 215123, PR China., Zaczek A; Laboratory of Translational Oncology Intercollegiate Faculty of Biotechnology Medical University of Gdansk Gda' nsk, 80-211, Poland., Liu J; Institute of Functional Nano and Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu Province, 215123, PR China. Electronic address: jliu@suda.edu.cn. |
| Πηγή: | Talanta [Talanta] 2026 Oct 01; Vol. 308, pp. 129868. Date of Electronic Publication: 2026 Apr 21. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: MEDLINE |
| Imprint Name(s): | Publication: Amsterdam : Elsevier Original Publication: Oxford : Pergamon Press |
| Ιατρικοί όροι (MeSH): | Vimentin*/analysis , Vimentin*/metabolism , Aptamers, Nucleotide*/chemistry , Single-Cell Analysis*/methods , Microarray Analysis*/methods, Biomarkers, Tumor/analysis ; Humans ; Cell Line, Tumor ; Epithelial-Mesenchymal Transition ; Limit of Detection ; MCF-7 Cells ; MDA-MB-231 Cells |
| Περίληψη: | Metastasis is a leading cause of cancer-related mortality, with epithelial-mesenchymal transition (EMT) playing a critical role in this process. Cell surface vimentin (CSV) has emerged as a promising biomarker for detecting aggressive and metastatic tumor cells, requiring optimized analytical methods for its detection. Herein, we report a platform that combines aptamer-mediated enzymatic cycling amplification with a superwettable droplet microarray (SDM) chip for sensitive CSV detection at the single-cell level. It features synergistic engineering of enzymatic nucleic acid cleavage for signal amplification and the confinement of the analytes within the ultrasmall volume of the microdroplet reactors on the chip. Our SDM assay achieves a limit of detection (LOD) as low as a single tumor cell and shows applicability across the tested cancer cell lines (MDA-MB-231, MCF-7, and PANC-1). Furthermore, it can detect CSV changes associated with TGF-β-induced phenotypic transition in a PANC-1 EMT model, and preserve cell proliferative capacity. This work provides a proof-of-concept approach for single-cell level CSV analysis, with the potential to support biomedical research on tumor cell sub-phenotypes and inform potential diagnostic strategies for personalized medicine. (Copyright © 2026 Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Cell surface vimentin; Enzymatic cycling amplification; Superwettable droplet microarrays |
| Substance Nomenclature: | 0 (Vimentin) 0 (Aptamers, Nucleotide) 0 (Biomarkers, Tumor) |
| Entry Date(s): | Date Created: 20260423 Date Completed: 20260715 Latest Revision: 20260715 |
| Update Code: | 20260716 |
| DOI: | 10.1016/j.talanta.2026.129868 |
| PMID: | 42025028 |
| Βάση Δεδομένων: | MEDLINE |
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