Academic Journal
Cytotoxicity and antibacterial BioEffects of CuO-Zn nanoparticles synthesized from Lactobacillus helveticus yogurt isolates targeting RsbA gene expression.
| Τίτλος: | Cytotoxicity and antibacterial BioEffects of CuO-Zn nanoparticles synthesized from Lactobacillus helveticus yogurt isolates targeting RsbA gene expression. |
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| Συγγραφείς: | Ahmed ME; Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq. mais.emad@sc.uobaghdad.edu.iq., Al Kubaisi WAH; Department of Basic Sciences, College of Dentistry, University of Kerbala, Kerbala, Iraq., Alhashimi HMM; Department of Microbiology, College of Medicine, University of Kerbala, Kerbala, Iraq., Alfaifi MY; Central Labs, King Khalid University, Alqura'a, Abha, Saudi Arabia.; Biology Department, Faculty of Science, King Khalid University, Abha, 9004, Saudi Arabia.; Tissue Culture and Cancer Biology Research Laboratory, King Khalid University, Abha, Saudi Arabia., Shati AA; Biology Department, Faculty of Science, King Khalid University, Abha, 9004, Saudi Arabia.; Tissue Culture and Cancer Biology Research Laboratory, King Khalid University, Abha, Saudi Arabia., Elbehairi SEI; Biology Department, Faculty of Science, King Khalid University, Abha, 9004, Saudi Arabia.; Tissue Culture and Cancer Biology Research Laboratory, King Khalid University, Abha, Saudi Arabia., Aufy M; Department of Pharmaceutical Sciences, Division of Pharmacology and Toxicology, University of Vienna, Vienna, Austria.; Department of Pharmaceutical Sciences, Division of Pharmaceutical Chemistry, University of Vienna, Vienna, Austria., Hussein AM; Department of Pharmaceutical Sciences, Division of Pharmacology and Toxicology, University of Vienna, Vienna, Austria. ahmed.hussein@univie.ac.at. |
| Πηγή: | Scientific reports [Sci Rep] 2026 Apr 22; Vol. 16 (1). Date of Electronic Publication: 2026 Apr 22. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: London : Nature Publishing Group, copyright 2011- |
| Ιατρικοί όροι (MeSH): | Anti-Bacterial Agents*/pharmacology , Anti-Bacterial Agents*/chemistry , Copper*/chemistry , Copper*/pharmacology , Lactobacillus helveticus*/metabolism , Lactobacillus helveticus*/isolation & purification , Lactobacillus helveticus*/chemistry , Yogurt*/microbiology , Metal Nanoparticles*/chemistry , Zinc Oxide*/chemistry , Zinc Oxide*/pharmacology , Proteus mirabilis*/drug effects , Bacterial Proteins*/genetics , Bacterial Proteins*/metabolism, Humans ; Microbial Sensitivity Tests ; Nanoparticles |
| Περίληψη: | Proteus mirabilis is an opportunistic urinary tract pathogen distinguished by its swarming motility and dimorphic behavior. This study examined the antibacterial potential of native Bulgarian dairy Lactobacillus strains against clinically significant P. mirabilis isolates. By developing and characterizing copper oxide (CuONPs) and zinc oxide (ZnONPs) nanoparticles as antimicrobial agents targeting P. mirabilis, this work contributes to addressing the global challenge of antimicrobial resistance, aligning with the United Nations Sustainable Development Goals (UNSDGs) that aim to reduce infectious disease mortality through innovative therapeutic approaches. Two multidrug-resistant P. mirabilis isolates (M1 and M2) displaying swarming motility were selected for analysis. The antimicrobial activity of Lactobacillus helveticus, a heterofermentative lactic acid bacterium commonly found in dairy products, was evaluated. This strain produces helveticin, a bacteriocin-like inhibitory substance (BLIS) with notable bactericidal activity. CuONPs and ZnONPs were biosynthesized using L. helveticus isolated from yogurt, with strain identity confirmed via 16 S rRNA gene sequencing. Nanoparticles were subsequently extracted and partially purified through 70% ammonium sulfate precipitation. Characterization revealed surface plasmon resonance peaks at 250 nm (CuONPs) and 300 nm (ZnONPs). AFM analysis indicated particle sizes ranging from 15 to 26 nm, while TEM imaging showed predominantly spherical to irregular morphologies between 9 and 20 nm. Cytotoxicity evaluation using the MTT assay demonstrated dose-dependent reductions in the viability of WRL68 human hepatic cells, with up to 60% inhibition at higher concentrations. Notably, both nanoparticle types effectively inhibited P. mirabilis swarming motility, and qPCR analysis confirmed significant downregulation of the rsbA gene, a crucial regulator of this behavior. Overall, green-synthesized CuONPs and ZnONPs derived from L. helveticus displayed potent anti-swarming and antivirulence effects against P. mirabilis. Their ability to suppress virulence-associated gene expression underscores their promise as eco-friendly antimicrobial agents for managing opportunistic pathogens and mitigating multidrug resistance. |
| Competing Interests: | Declarations. Competing interests: The authors declare no competing interests. |
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| Grant Information: | (CL/CO/B/3) Central labs at King Khalid University |
| Contributed Indexing: | Keywords: Copper nanoparticles; Green synthesized nanoparticles; L. helveticus; Zinc oxide nanoparticles |
| Substance Nomenclature: | 0 (Anti-Bacterial Agents) 789U1901C5 (Copper) SOI2LOH54Z (Zinc Oxide) 0 (Bacterial Proteins) |
| Entry Date(s): | Date Created: 20260422 Date Completed: 20260628 Latest Revision: 20260726 |
| Update Code: | 20260726 |
| PubMed Central ID: | PMC13272949 |
| DOI: | 10.1038/s41598-026-49023-y |
| PMID: | 42020702 |
| Βάση Δεδομένων: | MEDLINE |
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