Academic Journal
Targeted disruption of vacuolar-sorting protein and inhibitor of apoptosis genes via virus-induced gene silencing alters survival and reproduction of Diaphorina citri.
| Τίτλος: | Targeted disruption of vacuolar-sorting protein and inhibitor of apoptosis genes via virus-induced gene silencing alters survival and reproduction of Diaphorina citri. |
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| Συγγραφείς: | Killiny N; Department of Plant Pathology, Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, FL, USA., Rashidi M; Department of Plant Pathology, Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, FL, USA., Shilts T; Department of Plant Pathology, Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, FL, USA., Stelinski LL; Department of Entomology and Nematology, Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, FL, USA., Bonning BC; Department of Entomology and Nematology, Gainesville, IFAS, University of Florida, Gainesville, FL, USA. |
| Πηγή: | Pest management science [Pest Manag Sci] 2026 Aug; Vol. 82 (8), pp. 7596-7609. Date of Electronic Publication: 2026 Apr 20. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Published for SCI by Wiley Country of Publication: England NLM ID: 100898744 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1526-4998 (Electronic) Linking ISSN: 1526498X NLM ISO Abbreviation: Pest Manag Sci Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: West Sussex, UK : Published for SCI by Wiley, c2000- |
| Ιατρικοί όροι (MeSH): | Hemiptera*/genetics , Hemiptera*/physiology , Hemiptera*/growth & development , Hemiptera*/virology , Inhibitor of Apoptosis Proteins*/genetics , Inhibitor of Apoptosis Proteins*/metabolism , Insect Proteins*/genetics , Insect Proteins*/metabolism , Insect Control*/methods , Gene Silencing* , RNA Interference*, Nymph/growth & development ; Nymph/physiology ; Nymph/genetics ; Reproduction/genetics ; Animals ; Citrus ; Plant Diseases |
| Περίληψη: | Background: Citrus production worldwide is severely impacted by a devastating disease known as citrus greening, or Huanglongbing (HLB). The phytopathogen causing HLB is transmitted between trees by the Asian citrus psyllid, Diaphorina citri, which acts as the primary vector. Currently, there is no cure for HLB, and management efforts primarily rely on the use of insecticides. However, there is a growing need for alternative, environmentally sustainable control methods, such as RNA interference (RNAi). Results: We investigated the impact of gene silencing on the mortality of D. citri by targeting two genes, vacuolar-sorting protein/sucrose non-fermenting protein 7 (DcSnf7) and inhibitor of apoptosis 5 (DcIap5). Gene silencing was initially assessed by delivering synthesized double-stranded RNA (dsRNA) to D. citri through topical feeding. To further assess gene suppression in vivo, we used a virus-induced gene silencing (VIGS) approach to deliver RNAi to psyllids via citrus plants. Suppressing DcSnf7 and DcIap5 individually resulted in elevated nymph mortality; however, the combined suppression of both genes using dual dsRNA treatment did not yield an additive effect. We modified the infectious Citrus tristeza virus (CTV-T36) clone to individually and jointly carry the truncated genes, DcSnf7 and DcIap5. Over two successive generations, D. citri reared on plants inoculated with CTV-tSnf7, CTV-tIap5, or the combined construct CTV-tSnf7-tIap5 exhibited increased mortality at all life stages, as well as significantly reduced fecundity and fertility, compared to insects reared on non-infected or CTV-wt-inoculated control plants. In addition, these VIGS plants shortened the lifespan of D. citri. Notably, the dual construct CTV-tSnf7-tIap5 consistently produced the most pronounced reductions in survival, fecundity, fertility, and longevity of D. citri across all experiments, exceeding the effects observed with either single-gene construct. Conclusions: Our results suggest that silencing key genes of D. citri using RNAi mediated by VIGS represents a promising control strategy that could play a role in HLB management. © 2026 Society of Chemical Industry. (© 2026 Society of Chemical Industry.) |
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| Grant Information: | National Institute of Food and Agriculture |
| Contributed Indexing: | Keywords: Asian citrus psyllid; RNA interference; citrus greening disease; inhibitor of apoptosis (Iap5); vacuolar‐sorting protein (Snf7); virus‐induced gene silencing (VIGS) |
| Substance Nomenclature: | 0 (Inhibitor of Apoptosis Proteins) 0 (Insect Proteins) |
| SCR Organism: | Diaphorina citri |
| Entry Date(s): | Date Created: 20260421 Date Completed: 20260710 Latest Revision: 20260726 |
| Update Code: | 20260726 |
| PubMed Central ID: | PMC13352222 |
| DOI: | 10.1002/ps.70824 |
| PMID: | 42010772 |
| Βάση Δεδομένων: | MEDLINE |
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