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Functional suppression accompanies internal mitochondrial reorganization during cellular reprogramming.

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Τίτλος: Functional suppression accompanies internal mitochondrial reorganization during cellular reprogramming.
Συγγραφείς: Diak N; Department of Molecular Biology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Medykow 18 Street, 40-752, Katowice, Poland., Sieroń L; Department of Molecular Biology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Medykow 18 Street, 40-752, Katowice, Poland., Janelt K; Department of Molecular Biology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Medykow 18 Street, 40-752, Katowice, Poland., Chajec L; Faculty of Natural Sciences, Institute of Biology, Biotechnology and Environmental Protection, University of Silesia in Katowice, Bankowa 9, 40-007, Katowice, Poland., Fus-Kujawa A; Department of Molecular Biology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Medykow 18 Street, 40-752, Katowice, Poland., Dziedzic-Kowalska A; Department of Molecular Biology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Medykow 18 Street, 40-752, Katowice, Poland., Trybus A; Department of Molecular Biology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Medykow 18 Street, 40-752, Katowice, Poland., Raszczok K; Department of Molecular Biology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Medykow 18 Street, 40-752, Katowice, Poland.; Students Scientific Society, Faculty of Medical Sciences, Medical University of Silesia, Katowice, Poland., Bajdak-Rusinek K; Department of Molecular Biology, Faculty of Medical Sciences in Katowice, Medical University of Silesia, Medykow 18 Street, 40-752, Katowice, Poland. kbajdak-rusinek@sum.edu.pl.
Πηγή: Histochemistry and cell biology [Histochem Cell Biol] 2026 Apr 20; Vol. 164 (1). Date of Electronic Publication: 2026 Apr 20.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Springer Country of Publication: Germany NLM ID: 9506663 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-119X (Electronic) Linking ISSN: 09486143 NLM ISO Abbreviation: Histochem Cell Biol Subsets: MEDLINE
Imprint Name(s): Original Publication: Berlin : Springer
Ιατρικοί όροι (MeSH): Mitochondria*/metabolism , Induced Pluripotent Stem Cells*/metabolism , Induced Pluripotent Stem Cells*/cytology , Cellular Reprogramming*, Fibroblasts/metabolism ; Fibroblasts/cytology ; Reactive Oxygen Species/metabolism ; Humans ; Cells, Cultured ; Membrane Potential, Mitochondrial
Περίληψη: During the conversion of fibroblasts into induced pluripotent stem cells (iPSCs), cellular metabolism shifts from oxidative phosphorylation toward glycolysis; however, how functional and structural mitochondrial adaptations are coordinated remains incompletely understood. Here, we examined selected aspects of mitochondrial function, targeted gene expression, and ultrastructure in fibroblasts and iPSCs derived from healthy donors and patients with osteogenesis imperfecta (OI). Targeted gene expression analysis was performed in all four cell types, while functional and ultrastructural assays focused on OI-derived cells. Flow cytometry revealed reduced mitochondrial mass and reactive oxygen species (ROS) levels in iPSCs compared with fibroblasts. Mitochondrial membrane potential showed modestly reduced fluorescence depending on the probe used. Quantitative transmission electron microscopy demonstrated internal mitochondrial reorganization in iPSCs, including altered morphology and simplified cristae architecture, despite preservation of overall membrane integrity. These findings indicate that reprogramming induces coordinated functional downscaling and structural remodeling of mitochondria. Furthermore, differential expression of matrix metalloproteinases suggests that extracellular matrix remodeling accompanies metabolic adaptation during reprogramming.
(© 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Competing Interests: Declarations. Conflict of interest: The authors declare no competing interests.
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Grant Information: PCN-2-034/N/2/O Institutional funding from Medical University of Silesia; BNW-1-154/N/3/1 Institutional funding from Medical University of Silesia
Contributed Indexing: Keywords: Cellular reprogramming; Induced pluripotent stem cells; Metabolic remodeling; Mitochondrial function; Mitochondrial ultrastructure; Transmission electron microscopy
Substance Nomenclature: 0 (Reactive Oxygen Species)
Entry Date(s): Date Created: 20260420 Date Completed: 20260715 Latest Revision: 20260715
Update Code: 20260715
DOI: 10.1007/s00418-026-02472-y
PMID: 42010006
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1432-119X
DOI:10.1007/s00418-026-02472-y