Academic Journal
Efficient control of Tribulium castaneum using zeolitic imidazolate framework-8 nanocarrier-mediated delivery of dsRNA targeting Paramyosin.
| Title: | Efficient control of Tribulium castaneum using zeolitic imidazolate framework-8 nanocarrier-mediated delivery of dsRNA targeting Paramyosin. |
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| Authors: | Jiang L; Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling, China.; Shaanxi Provincial Center for Bio-Pesticide Engineering, Northwest A&F University, Yangling, China., Lu X; Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling, China.; Shaanxi Provincial Center for Bio-Pesticide Engineering, Northwest A&F University, Yangling, China., Jiang Y; Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling, China.; Shaanxi Provincial Center for Bio-Pesticide Engineering, Northwest A&F University, Yangling, China., Jin C; Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling, China.; Shaanxi Provincial Center for Bio-Pesticide Engineering, Northwest A&F University, Yangling, China., Wang L; School of Tropical Agriculture and Forestry, Hainan University, Haikou, China., Ma Z; Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling, China.; Shaanxi Provincial Center for Bio-Pesticide Engineering, Northwest A&F University, Yangling, China., Wu H; Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling, China.; Shaanxi Provincial Center for Bio-Pesticide Engineering, Northwest A&F University, Yangling, China. |
| Source: | Pest management science [Pest Manag Sci] 2026 Aug; Vol. 82 (8), pp. 7768-7779. Date of Electronic Publication: 2026 Apr 19. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Published for SCI by Wiley Country of Publication: England NLM ID: 100898744 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1526-4998 (Electronic) Linking ISSN: 1526498X NLM ISO Abbreviation: Pest Manag Sci Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: West Sussex, UK : Published for SCI by Wiley, c2000- |
| MeSH Terms: | Tribolium*/genetics , Tribolium*/growth & development , Tribolium*/drug effects , Insecticides*/pharmacology , Insect Control*/methods , Imidazoles*/chemistry , Insect Proteins*/genetics , Insect Proteins*/metabolism , RNA, Double-Stranded*, Larva/growth & development ; Larva/genetics ; Larva/drug effects ; Animals ; RNA Interference |
| Abstract: | Background: Tribolium castaneum is a major pest that affects stored grains globally. RNA insecticides present a promising strategy for the management of insect pests; however, they encounter challenges in target gene discovery, cost and efficiency. The paramyosin (PM) gene has shown potential as a target for RNAi-based insecticides. This study aims to explore the potential of ZIF-8@dsTcPM complexes as an RNA insecticide, assessing their effectiveness in targeting the TcPM gene while ensuring stability and protection of dsRNA. Results: This study discovered that the PM gene exists throughoutthe life cycle of T. castaneum and is predominantly located in adult muscle tissues. The dsTcPM notably downregulated TcPM gene expression, causing high mortalities in larvae and adults. The PM gene was highly conserved across diverse insect orders but absent in vertebrates, suggesting that the RNA insecticides targeting this gene may offer broad-spectrum control of insect pests with mammalian safety. The L4440-HT115 produced functional dsTcPM efficiently (2.15 μg mL-1) via fermentation under optimized conditions (0.8 mm IPTG at 37 °C for 6 h). ZIF-8 can spontaneously form stable complexes with dsTcPM with an average particle size of 125.64 nm to effectively protect dsTcPM from nuclease and hemolymph degradation. ZIF-8@dsTcPM not only exhibited safety for HEK293T cells, but also demonstrated significant toxicity to adult T. castaneum via the feeding method, with a mortality of 62.22% on the ninth day post-treatment. Conclusion: This work demonstrates that the ZIF-8@dsTcPM offers a promising option for the effective prevention and sustainable control of T. castaneum. © 2026 Society of Chemical Industry. (© 2026 Society of Chemical Industry.) |
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| Grant Information: | Open Fund Project of the Key Laboratory of Plant Protection Resources and Pest Management Northwest A&F University, Ministry of Education; China Postdoctoral Science Foundation; National Key Research and Development Program of China; National Natural Science Foundation of China |
| Contributed Indexing: | Keywords: Paramyosin; RNA insecticides; Tribolium castaneum; fermentation; nanomaterials |
| Substance Nomenclature: | 0 (Insecticides) 0 (RNA, Double-Stranded) 0 (Imidazoles) 0 (Insect Proteins) |
| Entry Date(s): | Date Created: 20260420 Date Completed: 20260710 Latest Revision: 20260726 |
| Update Code: | 20260726 |
| PubMed Central ID: | PMC13352346 |
| DOI: | 10.1002/ps.70839 |
| PMID: | 42002970 |
| Database: | MEDLINE |
| ISSN: | 1526-4998 |
|---|---|
| DOI: | 10.1002/ps.70839 |