Academic Journal
Integrative screening of plant volatiles through transient receptor potential channel docking, olfactometry, and field validation in Bemisia tabaci.
| Τίτλος: | Integrative screening of plant volatiles through transient receptor potential channel docking, olfactometry, and field validation in Bemisia tabaci. |
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| Συγγραφείς: | Paschapur AU; ICAR-Vivekananda Parvatiya Krishi Anusandhan Sansthan, Almora, India.; Division of Entomology, ICAR-Indian Agricultural Research Institute, New Delhi, India., Pandey D; Division of Entomology, ICAR-Indian Agricultural Research Institute, New Delhi, India., Singh L; Centre for Protected Cultivation Technology, ICAR-Indian Agricultural Research Institute, New Delhi, India., Marella SM; Division of Entomology, ICAR-Indian Agricultural Research Institute, New Delhi, India., Nebapure SM; Division of Entomology, ICAR-Indian Agricultural Research Institute, New Delhi, India., Subramanian S; Division of Entomology, ICAR-Indian Agricultural Research Institute, New Delhi, India. |
| Πηγή: | Pest management science [Pest Manag Sci] 2026 Aug; Vol. 82 (8), pp. 7463-7482. Date of Electronic Publication: 2026 Apr 16. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Published for SCI by Wiley Country of Publication: England NLM ID: 100898744 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1526-4998 (Electronic) Linking ISSN: 1526498X NLM ISO Abbreviation: Pest Manag Sci Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: West Sussex, UK : Published for SCI by Wiley, c2000- |
| Ιατρικοί όροι (MeSH): | Volatile Organic Compounds*/chemistry , Volatile Organic Compounds*/pharmacology , Transient Receptor Potential Channels*/metabolism , Transient Receptor Potential Channels*/chemistry , Transient Receptor Potential Channels*/genetics , Hemiptera*/physiology , Hemiptera*/drug effects , Insect Proteins*/metabolism , Insect Proteins*/chemistry , Insect Proteins*/genetics , Plants*/chemistry, Insect Control/methods ; Animals ; Molecular Docking Simulation ; Olfactometry ; RNA Interference |
| Περίληψη: | Background: Bemisia tabaci is a globally damaging pest for which insecticide reliance and resistance demand eco-rational, mechanism-informed alternatives. This study aimed to build a multi-tier pipeline of (TRP) channel docking, Y-tube olfactometry, RNA interference (RNAi) functional validation, and field trapping to identify and mechanistically support plant volatile attractants for improved monitoring/management. Results: Docking of 51 volatile organic compounds against six modelled TRP receptors (Transient Receptor Potential Cation Channel Subfamily A Member 1 (TRPA1), TRPV-Inactive, TRPL, Transient Receptor Potential Melastatin (TRPM), TRPML, TRPP-PKD1) yielded binding energies from -3.2 to -7.6 kcal mol-1, with TRPM/TRPA1 showing the most stable interactions; shortlisted ligands showed ΔG -5.9 to -7.6 kcal mol-1 and Ki 2.7-47.3 μM. In Y-tube assays (five concentrations: 10000-1 ppm, n = 5), attraction was ligand-dependent (F = 1852.03, P < 0.0001); top ligands reached A Conclusion: The convergence of docking affinity, lab attraction, RNAi-dependent behavioural suppression, and consistent field performance supports TRPA1/TRPM as key contributors to volatile-guided orientation and delivers validated attractants (notably thymol, β-caryophyllene, cuminaldehyde) for stronger, more reliable whitefly monitoring and semiochemical-based integrated pest management. © 2026 Society of Chemical Industry. (© 2026 Society of Chemical Industry.) |
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| Grant Information: | Indian Council of Agricultural Research |
| Contributed Indexing: | Keywords: RNA interference (RNAi); Y‐tube olfactometry; field trap evaluation; molecular docking; plant volatile organic compounds (VOCs); semiochemical‐based pest management; transient receptor potential (TRP) channels |
| Substance Nomenclature: | 0 (Volatile Organic Compounds) 0 (Transient Receptor Potential Channels) 0 (Insect Proteins) |
| Entry Date(s): | Date Created: 20260417 Date Completed: 20260710 Latest Revision: 20260813 |
| Update Code: | 20260813 |
| PubMed Central ID: | PMC13352296 |
| DOI: | 10.1002/ps.70812 |
| PMID: | 41992464 |
| Βάση Δεδομένων: | MEDLINE |
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