Academic Journal
Urease-null soybean (eu3-a) under salt and copper stress: nitrogen metabolism, antioxidant defense, and arginine pathway genes.
| Title: | Urease-null soybean (eu3-a) under salt and copper stress: nitrogen metabolism, antioxidant defense, and arginine pathway genes. |
|---|---|
| Authors: | de Souza SCR; Department of Plant Biology, Institute of Biology, University of Campinas, PO Box 6109, Campinas, SP, 13083-970, Brazil. sarahsouza@ufscar.br.; Department of Botany, Federal University of São Carlos, PO Box 676, São Carlos, SP, 13565-905, Brazil. sarahsouza@ufscar.br., Silveira NM; Department of Biodiversity, Institute of Biosciences, São Paulo State University (UNESP), PO Box 1515, Rio Claro, SP, 13506-900, Brazil., Tofanello VR; Department of Plant Biology, Institute of Biology, University of Campinas, PO Box 6109, Campinas, SP, 13083-970, Brazil., Polacco JC; Department of Biochemistry, Interdisciplinary Plant Group, University of Missouri, 117 Schweitzer Hall, Columbia, MO, 65211, USA., Mazzafera P; Department of Plant Biology, Institute of Biology, University of Campinas, PO Box 6109, Campinas, SP, 13083-970, Brazil. |
| Source: | Planta [Planta] 2026 Apr 16; Vol. 263 (5). Date of Electronic Publication: 2026 Apr 16. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Springer-Verlag [etc.] Country of Publication: Germany NLM ID: 1250576 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-2048 (Electronic) Linking ISSN: 00320935 NLM ISO Abbreviation: Planta Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Berlin, New York, Springer-Verlag [etc.] |
| MeSH Terms: | Glycine max*/genetics , Glycine max*/physiology , Glycine max*/drug effects , Glycine max*/enzymology , Glycine max*/metabolism , Nitrogen*/metabolism , Copper*/toxicity , Urease*/genetics , Urease*/metabolism , Antioxidants*/metabolism , Arginine*/metabolism, Photosynthesis/drug effects ; Sodium Chloride/pharmacology ; Gene Expression Regulation, Plant/drug effects ; Plant Leaves/physiology ; Salt Stress ; Stress, Physiological |
| Abstract: | Main Conclusion: The eu3-a mutant exhibited greater tolerance to salt stress but increased sensitivity to copper stress, with distinct impacts on nitrogen metabolism, photosynthesis, and antioxidant responses. The eu3-a soybean mutant is urease-null, lacking all urease activity responsible for catalyzing the hydrolysis of urea into ammonia and carbon dioxide. In this study, the urease-null eu3-a soybean mutant was used to assess the saline and copper stresses on nitrogen metabolism. Seeds of eu3-a/eu3-a and the corresponding dominant homozygous Eu3 precursor line were collectively referred to as near-isogenic lines (NILs). Experiments were conducted under hydroponic conditions using plants at the reproductive stage (R1-R2) and subjected to either salinity stress (NaCl: 0, 50, and 100 mM) or copper stress (CuCl (© 2026. The Author(s).) |
| Competing Interests: | Declarations. Conflict of interest: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
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| Grant Information: | 150069/2017-0 Conselho Nacional de Desenvolvimento Científico e Tecnológico; 2023/13662-3 Fundação de Amparo à Pesquisa do Estado de São Paulo; Grant 14/09730-4 Fundação de Amparo à Pesquisa do Estado de São Paulo |
| Contributed Indexing: | Keywords: Arginase; Nitrate; Polyamines; Proline; Tryptophan; Urea |
| Substance Nomenclature: | N762921K75 (Nitrogen) 789U1901C5 (Copper) EC 3.5.1.5 (Urease) 0 (Antioxidants) 94ZLA3W45F (Arginine) 451W47IQ8X (Sodium Chloride) |
| Entry Date(s): | Date Created: 20260416 Date Completed: 20260715 Latest Revision: 20260715 |
| Update Code: | 20260715 |
| PubMed Central ID: | PMC13086885 |
| DOI: | 10.1007/s00425-026-05001-2 |
| PMID: | 41989567 |
| Database: | MEDLINE |
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