Academic Journal
Micropatterning Substrates Using Deep UV Lithography: A Model to Study Host-Pathogen Interactions of A. fumigatus with Adherent Cells.
| Τίτλος: | Micropatterning Substrates Using Deep UV Lithography: A Model to Study Host-Pathogen Interactions of A. fumigatus with Adherent Cells. |
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| Συγγραφείς: | Polleux J; Research and Innovation Unit, Health University of Applied Sciences Tyrol, FH Gesundheit Tirol, Innsbruck, Austria. julien.polleux@fhg-tirol.ac.at.; Department of Ophthalmic Optics, Health University of Applied Sciences Tyrol, FH Gesundheit Tirol, Innsbruck, Austria. julien.polleux@fhg-tirol.ac.at., Heinrich L; Research and Innovation Unit, Health University of Applied Sciences Tyrol, FH Gesundheit Tirol, Innsbruck, Austria., Perkhofer S; Research and Innovation Unit, Health University of Applied Sciences Tyrol, FH Gesundheit Tirol, Innsbruck, Austria., Mach N; Research and Innovation Unit, Health University of Applied Sciences Tyrol, FH Gesundheit Tirol, Innsbruck, Austria. |
| Πηγή: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2026; Vol. 3028, pp. 167-177. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press |
| Ιατρικοί όροι (MeSH): | Aspergillus fumigatus*/physiology , Alveolar Epithelial Cells*/microbiology , Alveolar Epithelial Cells*/cytology , Host-Pathogen Interactions*, Cell Culture Techniques/methods ; Epithelial Cells/microbiology ; Ultraviolet Rays ; Humans ; Animals ; Cell Adhesion |
| Περίληψη: | Coculturing fungal pathogens with epithelial cell monolayers offers a powerful in vitro model for investigating host-pathogen interactions. Recent advances in bioengineering-particularly the use of micropatterned substrates-allow for the spatial confinement of alveolar epithelial cells into densely packed islands that closely resemble the structural organization of the flattened alveolus. This chapter outlines a comprehensive protocol that includes: (1) deep UV lithography for the fabrication of micropatterned glass coverslips, (2) culture of alveolar epithelial cells to generate uniform arrays of cell monolayer islands, and (3) infection with Aspergillus conidia to enable reliable quantification of host-pathogen dynamics. (© 2026. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
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| Contributed Indexing: | Keywords: Alveolar cells; Aspergillus fumigatus; Cell monolayer; Host-pathogen interactions; Micropatterning |
| Entry Date(s): | Date Created: 20260412 Date Completed: 20260714 Latest Revision: 20260714 |
| Update Code: | 20260715 |
| DOI: | 10.1007/978-1-0716-5222-0_12 |
| PMID: | 41968252 |
| Βάση Δεδομένων: | MEDLINE |
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