Academic Journal
Salicylic acid-mediated biosynthesis of anti-oomycete diterpenoids in Euphorbia lathyris L. suspension cells.
| Τίτλος: | Salicylic acid-mediated biosynthesis of anti-oomycete diterpenoids in Euphorbia lathyris L. suspension cells. |
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| Συγγραφείς: | Li P; Jiangsu Key Laboratory for Conservation and Utilization of Plant Resources, Jiangsu Province Engineering Research Center of Eco-Cultivation and High-Value Utilization of Chinese Medicinal Materials, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China., Sun X; Jiangsu Key Laboratory for Conservation and Utilization of Plant Resources, Jiangsu Province Engineering Research Center of Eco-Cultivation and High-Value Utilization of Chinese Medicinal Materials, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China., Zhang G; Jiangsu Key Laboratory for Conservation and Utilization of Plant Resources, Jiangsu Province Engineering Research Center of Eco-Cultivation and High-Value Utilization of Chinese Medicinal Materials, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China.; Nanjing University of Chinese Medicine, Nanjing, 210023, China., Wang B; Jiangsu Key Laboratory for Conservation and Utilization of Plant Resources, Jiangsu Province Engineering Research Center of Eco-Cultivation and High-Value Utilization of Chinese Medicinal Materials, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China., Xiao C; Jiangsu Key Laboratory for Conservation and Utilization of Plant Resources, Jiangsu Province Engineering Research Center of Eco-Cultivation and High-Value Utilization of Chinese Medicinal Materials, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China.; Nanjing University of Chinese Medicine, Nanjing, 210023, China., Tian M; Jiangsu Key Laboratory for Conservation and Utilization of Plant Resources, Jiangsu Province Engineering Research Center of Eco-Cultivation and High-Value Utilization of Chinese Medicinal Materials, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China., Xu S; Jiangsu Key Laboratory for Conservation and Utilization of Plant Resources, Jiangsu Province Engineering Research Center of Eco-Cultivation and High-Value Utilization of Chinese Medicinal Materials, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China., Liu H; Henan Jinling Honeysuckle Pharmaceutical Co., Ltd., Xinxiang Henan, 453300, China., Feng X; Jiangsu Key Laboratory for Conservation and Utilization of Plant Resources, Jiangsu Province Engineering Research Center of Eco-Cultivation and High-Value Utilization of Chinese Medicinal Materials, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China.; Nanjing University of Chinese Medicine, Nanjing, 210023, China., Li L; Jiangsu Key Laboratory for Conservation and Utilization of Plant Resources, Jiangsu Province Engineering Research Center of Eco-Cultivation and High-Value Utilization of Chinese Medicinal Materials, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China. xt_llw@163.com., Zhao W; Jiangsu Key Laboratory for Conservation and Utilization of Plant Resources, Jiangsu Province Engineering Research Center of Eco-Cultivation and High-Value Utilization of Chinese Medicinal Materials, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China. zhaowanlitcm@126.com., Chen Y; Jiangsu Key Laboratory for Conservation and Utilization of Plant Resources, Jiangsu Province Engineering Research Center of Eco-Cultivation and High-Value Utilization of Chinese Medicinal Materials, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China. ychen@jib.ac.cn.; Nanjing University of Chinese Medicine, Nanjing, 210023, China. ychen@jib.ac.cn. |
| Πηγή: | Plant cell reports [Plant Cell Rep] 2026 Apr 08; Vol. 45 (5). Date of Electronic Publication: 2026 Apr 08. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Springer Country of Publication: Germany NLM ID: 9880970 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-203X (Electronic) Linking ISSN: 07217714 NLM ISO Abbreviation: Plant Cell Rep Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Berlin ; New York : Springer, 1981- |
| Ιατρικοί όροι (MeSH): | Euphorbia*/metabolism , Euphorbia*/drug effects , Euphorbia*/genetics , Euphorbia*/cytology , Diterpenes*/metabolism , Diterpenes*/pharmacology , Diterpenes*/chemistry , Salicylic Acid*/pharmacology, Gene Expression Regulation, Plant/drug effects ; Plant Proteins/metabolism ; Plant Proteins/genetics ; Cells, Cultured |
| Περίληψη: | Key Message: Salicylic acid treatment significantly enhances the production of bioactive Euphorbia diterpenoids in Euphorbia lathyris suspension cultures by activating defense and diterpenoid biosynthetic pathways, including upregulating the key acyltransferase gene ElBAHD1. The medicinal plant Euphorbia lathyris produces pharmacologically active diterpenoids, but the content of which is limited in Euphorbia plants. An efficient suspension culture system of E. lathyris was established to enhance the production of bioactive diterpenoids. Salicylic acid (SA) treatment (550 μM, 10 days) significantly enhanced the production of lathyrane-type diterpenoids jolkinol A' (1), jolkinol A (2), jolkinol B (5), and a new 16-hydroxylated jolkinol A derivative (7). In particular, the volumetric yield of jolkinol A (2) was achieved to 47.00 mg L-1, a 5.4-fold increase over unelicited controls. Intriguingly, SA also induced the production of polycyclic diterpenoids, including ent-atisane and ent-kaurane. Transcriptomic analysis revealed SA activated defense and diterpenoid biosynthetic pathways, upregulating a series of genes including the acyltransferase gene ElBAHD1. Overexpression of ElBAHD1 promoted the accumulation of jolkinol A' (1), jolkinol A (2), and jolkinol B (5) in transgenic hairy roots and influenced the expression of diterpenoid biosynthetic genes. Notably, jolkinol A (2) exhibited potent antifungal activity against Phytophthora capsici, comparable to that of the commercial fungicide metalaxyl (84.76%) at 200 μg mL-1. This work provides insights into SA-induced biosynthesis mechanism of bioactive Euphorbia diterpenoids, and lays a robust foundation for the future metabolic engineering of E. lathyris. (© 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.) |
| Competing Interests: | Declarations. Conflict of interest: The authors declare no competing interests. |
| References: | Planta Med. 1984 Jun;50(3):259-61. (PMID: 6484031) Chem Biodivers. 2020 Feb;17(2):e1900531. (PMID: 31825561) Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9166-71. (PMID: 12089320) Molecules. 2022 May 09;27(9):. (PMID: 35566390) Front Plant Sci. 2018 Apr 27;9:554. (PMID: 29755494) Proc Natl Acad Sci U S A. 2016 Aug 23;113(34):E5082-9. (PMID: 27506796) Pest Manag Sci. 2021 Dec;77(12):5407-5417. (PMID: 34314099) ACS Med Chem Lett. 2021 Jun 25;12(7):1159-1165. (PMID: 34267886) Mol Cell. 2024 Jan 4;84(1):131-141. (PMID: 38103555) BMC Genomics. 2020 Aug 15;21(1):561. (PMID: 32799801) J Nat Prod. 1996 Nov;59(11):1051-5. (PMID: 8946746) Front Genet. 2021 Mar 29;12:657060. (PMID: 33854529) Ecotoxicol Environ Saf. 2021 Feb;209:111813. (PMID: 33360212) Chin J Nat Med. 2020 Dec;18(12):890-897. (PMID: 33357719) Plants (Basel). 2023 Sep 03;12(17):. (PMID: 37687411) Nat Prod Rep. 2022 Nov 16;39(11):2132-2174. (PMID: 36111621) J Ethnopharmacol. 2015 Dec 24;176:90-101. (PMID: 26485050) Pest Manag Sci. 2024 Mar;80(3):1228-1239. (PMID: 37897133) Nature. 2025 Sep;645(8079):208-217. (PMID: 40702180) Bioorg Chem. 2021 Jul;112:104944. (PMID: 33962090) Molecules. 2023 Mar 26;28(7):. (PMID: 37049722) Nature. 2025 Sep;645(8079):218-227. (PMID: 40702181) Org Lett. 2021 Dec 17;23(24):9602-9605. (PMID: 34816718) Org Biomol Chem. 2023 Aug 30;21(34):6949-6955. (PMID: 37581482) Bioorg Chem. 2020 Jan;95:103546. (PMID: 31911302) Plant Cell Tissue Organ Cult. 2023;153(3):447-458. (PMID: 37197003) Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):12715-20. (PMID: 12232048) Crit Rev Biotechnol. 2022 Feb;42(1):125-144. (PMID: 34151663) Appl Microbiol Biotechnol. 2024 Jun 19;108(1):382. (PMID: 38896329) Plant J. 2025 Feb;121(4):e70003. (PMID: 39968625) Front Plant Sci. 2021 Aug 09;12:697476. (PMID: 34434205) Phytochemistry. 2022 Jun;198:113142. (PMID: 35231502) Plant Physiol Biochem. 2018 Nov;132:271-280. (PMID: 30240989) Nature. 2025 Sep;645(8079):201-207. (PMID: 40702178) Biomed Pharmacother. 2022 May;149:112883. (PMID: 35692123) Phytochemistry. 2021 Oct;190:112846. (PMID: 34229224) Plant Physiol Biochem. 2023 Mar;196:431-443. (PMID: 36758290) Plant Commun. 2021 Aug 23;2(5):100235. (PMID: 34746764) New Phytol. 2025 Sep;247(6):2591-2600. (PMID: 40671640) Nat Prod Rep. 2010 Nov;27(11):1521-30. (PMID: 20890488) Proc Natl Acad Sci U S A. 2022 May 24;119(21):e2203890119. (PMID: 35584121) J Nat Prod. 2019 Jun 28;82(6):1634-1644. (PMID: 31180680) J Exp Bot. 2019 Aug 7;70(15):3969-3979. (PMID: 31120500) Nucleic Acids Res. 2017 Jan 4;45(D1):D1040-D1045. (PMID: 27924042) Int J Biol Macromol. 2025 Apr;302:139859. (PMID: 39814283) |
| Grant Information: | BE2022370 the Jiangsu Provincial Key Research and Development Program; 32300337 National Natural Science Foundation of China; 32200326 National Natural Science Foundation of China; 32270404 National Natural Science Foundation of China; JSPKLB202309 Open Fund of the Jiangsu Key Laboratory for Plant Resources Research and Utilization; JIBTF202304 Talent Fund of the Jiangsu Institute of Botany; JSPKLB202501 Scientific Fund of Nanjing Botanical Garden Mem. Sun Yat-Sen |
| Contributed Indexing: | Keywords: Euphorbia lathyris; Diterpenoids; Jolkinol A; Salicylic acid; Suspension cell |
| Substance Nomenclature: | 0 (Diterpenes) O414PZ4LPZ (Salicylic Acid) 0 (Plant Proteins) |
| Entry Date(s): | Date Created: 20260408 Date Completed: 20260714 Latest Revision: 20261010 |
| Update Code: | 20261010 |
| DOI: | 10.1007/s00299-026-03799-5 |
| PMID: | 41949771 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1432-203X |
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| DOI: | 10.1007/s00299-026-03799-5 |