Academic Journal
Surface-Initiated PET-RAFT Enables In Situ Glycopolymer Microarrays for Live-Cell Glycan Profiling and Programmable Biointerfaces.
| Τίτλος: | Surface-Initiated PET-RAFT Enables In Situ Glycopolymer Microarrays for Live-Cell Glycan Profiling and Programmable Biointerfaces. |
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| Συγγραφείς: | Mahdy AS; Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.; University of Chinese Academy of Sciences, Beijing 100049, China., Zhou J; Key Laboratory of Flexible Optoelectronic Materials and Technology (Ministry of Education), School of Optoelectronic Materials & Technology, Jianghan University, Wuhan 430056, China., Ni X; Key Laboratory of Flexible Optoelectronic Materials and Technology (Ministry of Education), School of Optoelectronic Materials & Technology, Jianghan University, Wuhan 430056, China., Chen Y; College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Road, Suzhou 215123, P. R. China., Gong J; Key Laboratory of Flexible Optoelectronic Materials and Technology (Ministry of Education), School of Optoelectronic Materials & Technology, Jianghan University, Wuhan 430056, China., Chen G; College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Road, Suzhou 215123, P. R. China., Geng J; Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.; Key Laboratory of Biomedical Imaging Science and System, Chinese Academy of Sciences, Shenzhen 518055, China.; State Key Laboratory of Biomedical Imaging Science and System, Shenzhen 518055, China.; Faculty of Pharmaceutical Sciences, Shenzhen University of Advanced Technology, Shenzhen 518107, China. |
| Πηγή: | Biomacromolecules [Biomacromolecules] 2026 Apr 13; Vol. 27 (4), pp. 2925-2937. Date of Electronic Publication: 2026 Apr 01. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 100892849 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1526-4602 (Electronic) Linking ISSN: 15257797 NLM ISO Abbreviation: Biomacromolecules Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Washington, DC : American Chemical Society, c2000- |
| Ιατρικοί όροι (MeSH): | Polysaccharides*/chemistry , Polysaccharides*/metabolism , Microarray Analysis*/methods , Polymers*/chemistry, Humans ; Hep G2 Cells ; Animals ; HeLa Cells ; Surface Properties ; Mice ; Cell Adhesion |
| Περίληψη: | Glycopolymer microarrays offer a powerful route to probe glycan-protein interactions, but existing "grafting-to" approaches require presynthesized polymers and provide limited control over surface density and multivalent presentation. Here we introduce a surface-initiated photoinduced electron-transfer RAFT (SI-PET-RAFT) platform that enables the in situ growth of well-defined glycopolymers directly on solid substrates under mild, oxygen-tolerant conditions. Using a combinatorial library of glycopolymers, we mapped the binding preferences of live HepG2, RAW264.7, and HeLa cells, revealing distinct and composition-dependent glycan-recognition signatures driven by multivalent effects. Guided by these insights, we designed thermoresponsive glycopolymer brushes on 96-well plates that mediate selective HepG2 adhesion at 37 °C and rapid, nondestructive release at 4 °C. This integrated workflow links high-throughput glycopolymer screening to the rational engineering of functional biointerfaces, offering broad opportunities in diagnostics, cell sorting, and glycan-targeted biomaterial design. |
| Substance Nomenclature: | 0 (Polysaccharides) 0 (Polymers) |
| Entry Date(s): | Date Created: 20260401 Date Completed: 20260714 Latest Revision: 20260714 |
| Update Code: | 20260714 |
| DOI: | 10.1021/acs.biomac.6c00229 |
| PMID: | 41919576 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1526-4602 |
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| DOI: | 10.1021/acs.biomac.6c00229 |