Academic Journal
Computational-guided semi-rational design to reprogram phospholipase D into industrially robust enzyme.
| Τίτλος: | Computational-guided semi-rational design to reprogram phospholipase D into industrially robust enzyme. |
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| Συγγραφείς: | Kong XL; Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, PR China; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China. Electronic address: 3171281291@qq.com., Zhang P; Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, PR China; National Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou 311300, PR China. Electronic address: 2652726703@qq.com., Qin ZQ; Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, PR China; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China. Electronic address: 76674326@qq.com., Su C; Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, PR China; Institute of Future Food Technology, JITRI, Yixing 214200, PR China. Electronic address: 174020590@qq.com., Li H; Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, PR China; Institute of Future Food Technology, JITRI, Yixing 214200, PR China. Electronic address: liheng@jiangnan.edu.cn., Lu ZM; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China. Electronic address: zmlu@jiangnan.edu.cn., Gong JS; Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, PR China; Institute of Future Food Technology, JITRI, Yixing 214200, PR China; Shandong Kunda Biotechnology Co., Ltd, Linyi 276400, PR China. Electronic address: jinsonggong.bio@hotmail.com., Xu ZH; Institute of Future Food Technology, JITRI, Yixing 214200, PR China; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China; Innovation Center for Advanced Brewing Science and Technology, College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, PR China. Electronic address: zhenghxu@jiangnan.edu.cn., Shi JS; Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, PR China; Institute of Future Food Technology, JITRI, Yixing 214200, PR China. Electronic address: shijs@163.com. |
| Πηγή: | Bioresource technology [Bioresour Technol] 2026 Jul; Vol. 451, pp. 134528. Date of Electronic Publication: 2026 Mar 29. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Applied Science Country of Publication: England NLM ID: 9889523 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2976 (Electronic) Linking ISSN: 09608524 NLM ISO Abbreviation: Bioresour Technol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991- |
| Ιατρικοί όροι (MeSH): | Phospholipase D*/chemistry , Phospholipase D*/genetics , Phospholipase D*/metabolism , Protein Engineering*/methods , Computer-Aided Design*, Mutation/genetics ; Molecular Dynamics Simulation ; Enzyme Stability ; Temperature ; Thermodynamics |
| Περίληψη: | Phospholipase D (PLD) catalyzes the synthesis of rare phospholipid derivatives from phospholipids, and these derivatives possess significant application value and market potential. However, most reported PLDs show poor thermal stability, with melting temperatures below 60 °C, limiting their industrial application. This study employed a computer-aided design strategy integrating ancestral sequence reconstruction (ASR) and Gibbs folding free energy (ΔΔG) calculation to enhance PLD's thermal stability. Through iterative accumulation mutagenesis, a combinatorial mutant PLD (Copyright © 2026 Elsevier Ltd. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Ancestral enzyme residue substitution; Energy calculation; Molecular dynamics simulation; Phosphatidylserine; Thermal stability modification |
| Substance Nomenclature: | EC 3.1.4.4 (Phospholipase D) |
| Entry Date(s): | Date Created: 20260331 Date Completed: 20260714 Latest Revision: 20260714 |
| Update Code: | 20260714 |
| DOI: | 10.1016/j.biortech.2026.134528 |
| PMID: | 41916458 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-2976 |
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| DOI: | 10.1016/j.biortech.2026.134528 |