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Nitrospira-Driven partial Denitrification: Kinetics, Mechanisms, and synergistic potential with anammox.

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Τίτλος: Nitrospira-Driven partial Denitrification: Kinetics, Mechanisms, and synergistic potential with anammox.
Συγγραφείς: Wang J; School of Environment and Climate, Guangdong Engineering Research Center of Water Treatment Processes and Materials, and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 510632, China., Huang J; South China Institute of Environmental Science, Ministry of Ecology and Environment, Guangdong Provincial Key Laboratory of Water and Air Pollution Control, State Key Laboratory of Water Environment Simulation and Pollution Control, Guangzhou 51053, China., Li D; School of Environment and Climate, Guangdong Engineering Research Center of Water Treatment Processes and Materials, and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 510632, China., Guo Y; School of Environment and Climate, Guangdong Engineering Research Center of Water Treatment Processes and Materials, and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 510632, China., Zeng Y; School of Environment and Climate, Guangdong Engineering Research Center of Water Treatment Processes and Materials, and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 510632, China., Liu G; School of Environment and Climate, Guangdong Engineering Research Center of Water Treatment Processes and Materials, and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 510632, China; South China Institute of Environmental Science, Ministry of Ecology and Environment, Guangdong Provincial Key Laboratory of Water and Air Pollution Control, State Key Laboratory of Water Environment Simulation and Pollution Control, Guangzhou 51053, China. Electronic address: gqliu@jnu.edu.cn.
Πηγή: Bioresource technology [Bioresour Technol] 2026 Jul; Vol. 451, pp. 134530. Date of Electronic Publication: 2026 Mar 29.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Elsevier Applied Science Country of Publication: England NLM ID: 9889523 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2976 (Electronic) Linking ISSN: 09608524 NLM ISO Abbreviation: Bioresour Technol Subsets: MEDLINE
Imprint Name(s): Original Publication: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991-
Ιατρικοί όροι (MeSH): Denitrification*/physiology , Denitrification*/drug effects , Bacteria*/metabolism , Bacteria*/genetics , Anaerobic Ammonia Oxidation*, Nitrites/metabolism ; Formates/metabolism ; Nitrogen/metabolism ; Nitrates/metabolism ; Kinetics ; Biomass ; Oxidation-Reduction
Περίληψη: In nitrite-oxidizing bacteria (NOB)-enriched biomass, formate (3 mM) promoted partial denitrification (PD) by NOB-Nitrospira, resulting in a nitrite accumulation rate of 1.02 ± 0.17 mg-N/(g-SS·h). The relationship between nitrite accumulation rate and formate concentration followed Michaelis-Menten kinetics (R2 = 0.95), with a half-saturation constant of 3.18 mM. The Nitrospira in NOB biomass harbored fdh gene (encoding formate dehydrogenase) and nitrate reduction genes narG and napA, which were upregulated by 137.0%, 5.5%, and 127.0% with formate addition, respectively. This revealed a synergy between formate oxidation (electron donation) via fdh and nitrate reduction, thereby enabling PD. At 3 mM formate, the addition of NOB into anaerobic ammonium oxidizing bacteria (AnAOB) biomass increased the nitrogen removal rate from 1.74 to 3.56 mg-N/(g-SS·h), demonstrating effective coupling between NOB-driven PD and anammox. This study reveals that NOB Nitrospira can shift from a competitor to a collaborator with AnAOB, offering a potential new pathway for nitrogen removal.
(Copyright © 2026 Elsevier Ltd. All rights reserved.)
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Contributed Indexing: Keywords: Anaerobic ammonia oxidation; Formate; Microbial synergy; Nitrate reduction; Nitrite-oxidizing bacteria
Substance Nomenclature: 0 (Nitrites)
0 (Formates)
N762921K75 (Nitrogen)
0 (Nitrates)
0YIW783RG1 (formic acid)
Entry Date(s): Date Created: 20260331 Date Completed: 20260714 Latest Revision: 20260714
Update Code: 20260714
DOI: 10.1016/j.biortech.2026.134530
PMID: 41916454
Βάση Δεδομένων: MEDLINE
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  Data: Nitrospira-Driven partial Denitrification: Kinetics, Mechanisms, and synergistic potential with anammox.
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  Data: <searchLink fieldCode="AU" term="%22Wang+J%22">Wang J</searchLink>; School of Environment and Climate, Guangdong Engineering Research Center of Water Treatment Processes and Materials, and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 510632, China.<br /><searchLink fieldCode="AU" term="%22Huang+J%22">Huang J</searchLink>; South China Institute of Environmental Science, Ministry of Ecology and Environment, Guangdong Provincial Key Laboratory of Water and Air Pollution Control, State Key Laboratory of Water Environment Simulation and Pollution Control, Guangzhou 51053, China.<br /><searchLink fieldCode="AU" term="%22Li+D%22">Li D</searchLink>; School of Environment and Climate, Guangdong Engineering Research Center of Water Treatment Processes and Materials, and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 510632, China.<br /><searchLink fieldCode="AU" term="%22Guo+Y%22">Guo Y</searchLink>; School of Environment and Climate, Guangdong Engineering Research Center of Water Treatment Processes and Materials, and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 510632, China.<br /><searchLink fieldCode="AU" term="%22Zeng+Y%22">Zeng Y</searchLink>; School of Environment and Climate, Guangdong Engineering Research Center of Water Treatment Processes and Materials, and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 510632, China.<br /><searchLink fieldCode="AU" term="%22Liu+G%22">Liu G</searchLink>; School of Environment and Climate, Guangdong Engineering Research Center of Water Treatment Processes and Materials, and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 510632, China; South China Institute of Environmental Science, Ministry of Ecology and Environment, Guangdong Provincial Key Laboratory of Water and Air Pollution Control, State Key Laboratory of Water Environment Simulation and Pollution Control, Guangzhou 51053, China. Electronic address: gqliu@jnu.edu.cn.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Elsevier+Applied+Science%22">Elsevier Applied Science </searchLink><i>Country of Publication: </i>England <i>NLM ID: </i>9889523 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1873-2976 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2209608524%22">09608524 </searchLink><i>NLM ISO Abbreviation: </i>Bioresour Technol <i>Subsets: </i>MEDLINE
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  Data: <i>Original Publication</i>: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991-
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  Data: <searchLink fieldCode="MM" term="%22Denitrification%22">Denitrification*</searchLink>/<searchLink fieldCode="MM" term="%22Denitrification+physiology%22">physiology</searchLink> <br /><searchLink fieldCode="MM" term="%22Denitrification%22">Denitrification*</searchLink>/<searchLink fieldCode="MM" term="%22Denitrification+drug+effects%22">drug effects</searchLink> <br /><searchLink fieldCode="MM" term="%22Bacteria%22">Bacteria*</searchLink>/<searchLink fieldCode="MM" term="%22Bacteria+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Bacteria%22">Bacteria*</searchLink>/<searchLink fieldCode="MM" term="%22Bacteria+genetics%22">genetics</searchLink> <br /><searchLink fieldCode="MM" term="%22Anaerobic+Ammonia+Oxidation%22">Anaerobic Ammonia Oxidation*</searchLink><br /><searchLink fieldCode="MH" term="%22Nitrites%22">Nitrites</searchLink>/<searchLink fieldCode="MH" term="%22Nitrites+metabolism%22">metabolism</searchLink> ; <searchLink fieldCode="MH" term="%22Formates%22">Formates</searchLink>/<searchLink fieldCode="MH" term="%22Formates+metabolism%22">metabolism</searchLink> ; <searchLink fieldCode="MH" term="%22Nitrogen%22">Nitrogen</searchLink>/<searchLink fieldCode="MH" term="%22Nitrogen+metabolism%22">metabolism</searchLink> ; <searchLink fieldCode="MH" term="%22Nitrates%22">Nitrates</searchLink>/<searchLink fieldCode="MH" term="%22Nitrates+metabolism%22">metabolism</searchLink> ; <searchLink fieldCode="MH" term="%22Kinetics%22">Kinetics</searchLink> ; <searchLink fieldCode="MH" term="%22Biomass%22">Biomass</searchLink> ; <searchLink fieldCode="MH" term="%22Oxidation-Reduction%22">Oxidation-Reduction</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: In nitrite-oxidizing bacteria (NOB)-enriched biomass, formate (3 mM) promoted partial denitrification (PD) by NOB-Nitrospira, resulting in a nitrite accumulation rate of 1.02 ± 0.17 mg-N/(g-SS·h). The relationship between nitrite accumulation rate and formate concentration followed Michaelis-Menten kinetics (R<superscript>2</superscript> = 0.95), with a half-saturation constant of 3.18 mM. The Nitrospira in NOB biomass harbored fdh gene (encoding formate dehydrogenase) and nitrate reduction genes narG and napA, which were upregulated by 137.0%, 5.5%, and 127.0% with formate addition, respectively. This revealed a synergy between formate oxidation (electron donation) via fdh and nitrate reduction, thereby enabling PD. At 3 mM formate, the addition of NOB into anaerobic ammonium oxidizing bacteria (AnAOB) biomass increased the nitrogen removal rate from 1.74 to 3.56 mg-N/(g-SS·h), demonstrating effective coupling between NOB-driven PD and anammox. This study reveals that NOB Nitrospira can shift from a competitor to a collaborator with AnAOB, offering a potential new pathway for nitrogen removal.<br /> (Copyright © 2026 Elsevier Ltd. All rights reserved.)
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  Label: Competing Interests
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  Data: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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  Data: <i>Keywords: </i>Anaerobic ammonia oxidation; Formate; Microbial synergy; Nitrate reduction; Nitrite-oxidizing bacteria
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  Data: 0 (Nitrites)<br />0 (Formates)<br />N762921K75 (Nitrogen)<br />0 (Nitrates)<br />0YIW783RG1 (formic acid)
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        Value: 10.1016/j.biortech.2026.134530
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        Text: English
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        StartPage: 134530
    Subjects:
      – SubjectFull: Nitrites metabolism
        Type: general
      – SubjectFull: Formates metabolism
        Type: general
      – SubjectFull: Nitrogen metabolism
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      – SubjectFull: Nitrates metabolism
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      – TitleFull: Nitrospira-Driven partial Denitrification: Kinetics, Mechanisms, and synergistic potential with anammox.
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