Academic Journal
Structure and function of mouse lens suture examined by 2-photon fluorescence microscopic imaging.
| Τίτλος: | Structure and function of mouse lens suture examined by 2-photon fluorescence microscopic imaging. |
|---|---|
| Συγγραφείς: | Zhang Q; Department of Physics, University of California, Berkeley, CA, 94720, USA.; Department of Biomedical Engineering, College of Biomedicine, City University of Hong Kong, Kowloon, Hong Kong SAR, China., Zhu J; Department of Neuroscience, University of California, Berkeley, CA, 94720, USA., Painter T; Herbert Wertheim School of Optometry, University of California, Berkeley, CA, 94720, USA.; Vision Science Program, University of California, Berkeley, CA, 94720, USA., Xia CH; Herbert Wertheim School of Optometry, University of California, Berkeley, CA, 94720, USA.; Vision Science Program, University of California, Berkeley, CA, 94720, USA., Ji N; Department of Physics, University of California, Berkeley, CA, 94720, USA.; Department of Neuroscience, University of California, Berkeley, CA, 94720, USA.; Helen Wills Neuroscience Institute, University of California, Berkeley, CA, 94720, USA.; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Gong X; Herbert Wertheim School of Optometry, University of California, Berkeley, CA, 94720, USA. xgong@berkeley.edu.; Vision Science Program, University of California, Berkeley, CA, 94720, USA. xgong@berkeley.edu. |
| Πηγή: | Scientific reports [Sci Rep] 2026 Mar 24; Vol. 16 (1). Date of Electronic Publication: 2026 Mar 24. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: London : Nature Publishing Group, copyright 2011- |
| Ιατρικοί όροι (MeSH): | Lens, Crystalline*/diagnostic imaging , Lens, Crystalline*/metabolism , Lens, Crystalline*/pathology , Lens, Crystalline*/ultrastructure , Cataract*/pathology , Cataract*/diagnostic imaging , Microscopy, Fluorescence, Multiphoton*/methods, Animals ; Mice ; Mice, Knockout ; Microscopy, Fluorescence |
| Περίληψη: | We used two-photon fluorescence microscopy (2PFM) to examine lens fiber and suture architecture, as well as permeability, in wild-type (WT) lenses and in lenses from klotho-like protein homology (KLPH) knockout (KLPH-KO) mice. KLPH is a type I membrane glycoprotein encoded by the Lctl gene; KLPH-KO mice develop lens suture cataracts. Lens sutures have been hypothesized to serve as pathways for transporting ions, nutrients, and other factors as part of the lens microcirculation required to maintain homeostasis and transparency. Three-dimensional (3D) in vivo imaging revealed typical "Y" and "double-Y" anterior suture geometries, along with depth-dependent variation, in WT lenses. In contrast, KLPH-KO lenses exhibited markedly heterogeneous suture morphologies and misalignment between the anterior and posterior suture planes. Quantification of pattern variability using the mean structural similarity index (SSIM) of individual z-stacks relative to the stack mean projection revealed a significant increase in pattern randomization in KLPH-KO lenses (p < 0.05). Both WT and KLPH-KO lenses displayed voids near sutures and enlarged vacuoles distributed throughout the lens in vivo. Notably, KLPH-KO lenses exhibited irregular, enlarged central voids containing ~ 2-5 μm amorphous structures, consistent with subcellular remnants and/or membrane-associated aggregates. In conclusion, this study provides novel morphological markers for characterizing suture cataracts and associated fiber pathology. It further demonstrates that the dye-impermeable lens suture represents a stabilized interface formed by elongated fiber ends, supporting lens integrity and maintaining the organization of lens fibers. (© 2026. The Author(s).) |
| Competing Interests: | Declarations. Competing interests: The authors declare no competing interests. |
| Σχόλια: | Update of: bioRxiv. 2025 Nov 07:2025.09.08.675023. doi: 10.1101/2025.09.08.675023.. (PMID: 41030987) |
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| Grant Information: | T32 EY007043 United States EY NEI NIH HHS; U01 NS118300 United States NS NINDS NIH HHS |
| Entry Date(s): | Date Created: 20260325 Date Completed: 20260713 Latest Revision: 20260726 |
| Update Code: | 20260726 |
| PubMed Central ID: | PMC13168428 |
| DOI: | 10.1038/s41598-026-45299-2 |
| PMID: | 41876809 |
| Βάση Δεδομένων: | MEDLINE |
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