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Cytocompatibility and influence on dentin permeability of galactomannan from Caesalpinia ferrea seeds.

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Τίτλος: Cytocompatibility and influence on dentin permeability of galactomannan from Caesalpinia ferrea seeds.
Συγγραφείς: Rabelo CS; Department of Clinical Dentistry, School of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, CE, Brazil., Rocha AVC; Department of Clinical Dentistry, School of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, CE, Brazil., Marques VM; Department of Clinical Dentistry, School of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, CE, Brazil., Mejía VC; Department of Clinical Dentistry, School of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, CE, Brazil., Rodrigues FAM; Department of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza, CE, Brazil., Ricardo NMPS; Department of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza, CE, Brazil., Silva PGB; Department of Dentistry, Unichristus, Fortaleza, CE, Brazil., Mokeem LS; Program in Dental Biomedical Sciences, University of Maryland School of Dentistry, Baltimore, MD, USA., Melo MAS; Division of Operative Dentistry, Department of General Dentistry, University of Maryland School of Dentistry, Baltimore, MD, USA., Passos VF; Department of Restorative Dentistry, School of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, CE, Brazil. Electronic address: vanarapassos@ufc.br.
Πηγή: Archives of oral biology [Arch Oral Biol] 2026 Jun; Vol. 186, pp. 106573. Date of Electronic Publication: 2026 Mar 12.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Pergamon Press Country of Publication: England NLM ID: 0116711 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1506 (Electronic) Linking ISSN: 00039969 NLM ISO Abbreviation: Arch Oral Biol Subsets: MEDLINE
Imprint Name(s): Publication: Oxford : Pergamon Press
Original Publication: Oxford, New York [etc.] Pergamon Press.
Ιατρικοί όροι (MeSH): Caesalpinia*/chemistry , Seeds*/chemistry , Mannans*/pharmacology , Dentin Permeability*/drug effects , Dentin*/drug effects, Galactose/analogs & derivatives ; Fibroblasts/drug effects ; Dental Pulp/cytology ; Dental Pulp/drug effects ; Gingiva/cytology ; Gingiva/drug effects ; Cell Survival/drug effects ; Humans ; Microscopy, Electron, Scanning
Περίληψη: Objectives: To investigate the effects of galactomannan extracted from Caesalpinia ferrea seeds on dentin permeability and its cytocompatibility with oral cells.
Design: Sixty dentin discs were treated with EDTA for 5 min and, after determining maximum permeability, randomly assigned to four groups (n = 12): distilled water (DW), potassium oxalate (PO), 1% juca seed galactomannan (JSG1), and 2% juca seed galactomannan (JSG2). Dentin permeability was measured again after treatment and after a 5-day erosive-abrasive cycle (four daily immersions in 0.3% citric acid for 5 min, followed by 60 min in artificial saliva, with brushing after the first and last challenges). Treatments were reapplied daily. The percentage of dentin permeability (%Lp) was calculated relative to maximum permeability. Surface morphology was examined by scanning electron microscopy (SEM), and cytotoxicity was tested in human gingival fibroblasts (HGF) and dental pulp stem cells (DPSC). Data were analyzed using ANOVA and Bonferroni test (p < 0.05).
Results: After treatment, no significant differences in permeability were observed among groups (p > 0.05). Following cycling, JSG2 showed significantly lower permeability than DW (p < 0.05). Only DW exhibited a significant increase in permeability over time (p < 0.05). SEM images revealed that PO and JSG2 maintained partially obliterated tubules even after cycling. Cytotoxicity assays showed cell viability near 100% for HGF and 88% for DPSC at the highest juca seed galactomannan (JSG) concentration, confirming cytocompatibility.
Conclusions: JSG at 2% concentration partially occluded dentinal tubules, preserved dentin permeability under erosive-abrasive conditions, and demonstrated cytocompatibility with HGF and DPSC.
(Copyright © 2026 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Contributed Indexing: Keywords: Caesalpinia; Cytotoxicity; Dentin hypersensitivity; Dentin permeability; Tubule occlusion
Substance Nomenclature: X2RN3Q8DNE (Galactose)
11078-30-1 (galactomannan)
0 (Mannans)
Entry Date(s): Date Created: 20260318 Date Completed: 20260711 Latest Revision: 20260711
Update Code: 20260711
DOI: 10.1016/j.archoralbio.2026.106573
PMID: 41849957
Βάση Δεδομένων: MEDLINE
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  Data: Cytocompatibility and influence on dentin permeability of galactomannan from Caesalpinia ferrea seeds.
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  Data: &lt;searchLink fieldCode=&quot;AU&quot; term=&quot;%22Rabelo+CS%22&quot;&gt;Rabelo CS&lt;/searchLink&gt;; Department of Clinical Dentistry, School of Pharmacy, Dentistry and Nursing, Federal University of Cear&#225;, Fortaleza, CE, Brazil.&lt;br /&gt;&lt;searchLink fieldCode=&quot;AU&quot; term=&quot;%22Rocha+AVC%22&quot;&gt;Rocha AVC&lt;/searchLink&gt;; Department of Clinical Dentistry, School of Pharmacy, Dentistry and Nursing, Federal University of Cear&#225;, Fortaleza, CE, Brazil.&lt;br /&gt;&lt;searchLink fieldCode=&quot;AU&quot; term=&quot;%22Marques+VM%22&quot;&gt;Marques VM&lt;/searchLink&gt;; Department of Clinical Dentistry, School of Pharmacy, Dentistry and Nursing, Federal University of Cear&#225;, Fortaleza, CE, Brazil.&lt;br /&gt;&lt;searchLink fieldCode=&quot;AU&quot; term=&quot;%22Mej&#237;a+VC%22&quot;&gt;Mej&#237;a VC&lt;/searchLink&gt;; Department of Clinical Dentistry, School of Pharmacy, Dentistry and Nursing, Federal University of Cear&#225;, Fortaleza, CE, Brazil.&lt;br /&gt;&lt;searchLink fieldCode=&quot;AU&quot; term=&quot;%22Rodrigues+FAM%22&quot;&gt;Rodrigues FAM&lt;/searchLink&gt;; Department of Organic and Inorganic Chemistry, Federal University of Cear&#225;, Fortaleza, CE, Brazil.&lt;br /&gt;&lt;searchLink fieldCode=&quot;AU&quot; term=&quot;%22Ricardo+NMPS%22&quot;&gt;Ricardo NMPS&lt;/searchLink&gt;; Department of Organic and Inorganic Chemistry, Federal University of Cear&#225;, Fortaleza, CE, Brazil.&lt;br /&gt;&lt;searchLink fieldCode=&quot;AU&quot; term=&quot;%22Silva+PGB%22&quot;&gt;Silva PGB&lt;/searchLink&gt;; Department of Dentistry, Unichristus, Fortaleza, CE, Brazil.&lt;br /&gt;&lt;searchLink fieldCode=&quot;AU&quot; term=&quot;%22Mokeem+LS%22&quot;&gt;Mokeem LS&lt;/searchLink&gt;; Program in Dental Biomedical Sciences, University of Maryland School of Dentistry, Baltimore, MD, USA.&lt;br /&gt;&lt;searchLink fieldCode=&quot;AU&quot; term=&quot;%22Melo+MAS%22&quot;&gt;Melo MAS&lt;/searchLink&gt;; Division of Operative Dentistry, Department of General Dentistry, University of Maryland School of Dentistry, Baltimore, MD, USA.&lt;br /&gt;&lt;searchLink fieldCode=&quot;AU&quot; term=&quot;%22Passos+VF%22&quot;&gt;Passos VF&lt;/searchLink&gt;; Department of Restorative Dentistry, School of Pharmacy, Dentistry and Nursing, Federal University of Cear&#225;, Fortaleza, CE, Brazil. Electronic address: vanarapassos@ufc.br.
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– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Objectives: To investigate the effects of galactomannan extracted from Caesalpinia ferrea seeds on dentin permeability and its cytocompatibility with oral cells.&lt;br /&gt;Design: Sixty dentin discs were treated with EDTA for 5 min and, after determining maximum permeability, randomly assigned to four groups (n = 12): distilled water (DW), potassium oxalate (PO), 1% juca seed galactomannan (JSG1), and 2% juca seed galactomannan (JSG2). Dentin permeability was measured again after treatment and after a 5-day erosive-abrasive cycle (four daily immersions in 0.3% citric acid for 5 min, followed by 60 min in artificial saliva, with brushing after the first and last challenges). Treatments were reapplied daily. The percentage of dentin permeability (%Lp) was calculated relative to maximum permeability. Surface morphology was examined by scanning electron microscopy (SEM), and cytotoxicity was tested in human gingival fibroblasts (HGF) and dental pulp stem cells (DPSC). Data were analyzed using ANOVA and Bonferroni test (p &amp;lt; 0.05).&lt;br /&gt;Results: After treatment, no significant differences in permeability were observed among groups (p &amp;gt; 0.05). Following cycling, JSG2 showed significantly lower permeability than DW (p &amp;lt; 0.05). Only DW exhibited a significant increase in permeability over time (p &amp;lt; 0.05). SEM images revealed that PO and JSG2 maintained partially obliterated tubules even after cycling. Cytotoxicity assays showed cell viability near 100% for HGF and 88% for DPSC at the highest juca seed galactomannan (JSG) concentration, confirming cytocompatibility.&lt;br /&gt;Conclusions: JSG at 2% concentration partially occluded dentinal tubules, preserved dentin permeability under erosive-abrasive conditions, and demonstrated cytocompatibility with HGF and DPSC.&lt;br /&gt; (Copyright &#169; 2026 The Authors. Published by Elsevier Ltd.. All rights reserved.)
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  Data: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
– Name: SubjectMinor
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  Data: &lt;i&gt;Keywords: &lt;/i&gt;Caesalpinia; Cytotoxicity; Dentin hypersensitivity; Dentin permeability; Tubule occlusion
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  Label: Substance Nomenclature
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  Data: X2RN3Q8DNE (Galactose)&lt;br /&gt;11078-30-1 (galactomannan)&lt;br /&gt;0 (Mannans)
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        Text: English
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        StartPage: 106573
    Subjects:
      – SubjectFull: Galactose analogs & derivatives
        Type: general
      – SubjectFull: Fibroblasts drug effects
        Type: general
      – SubjectFull: Dental Pulp cytology
        Type: general
      – SubjectFull: Dental Pulp drug effects
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      – SubjectFull: Humans
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      – SubjectFull: Microscopy, Electron, Scanning
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      – SubjectFull: Caesalpinia chemistry
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      – SubjectFull: Dentin drug effects
        Type: general
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