Academic Journal
Combining Yeast Display and Bacterial Genomic Library for the Unbiased Isolation of Novel Polysaccharide-Binding Peptides.
| Τίτλος: | Combining Yeast Display and Bacterial Genomic Library for the Unbiased Isolation of Novel Polysaccharide-Binding Peptides. |
|---|---|
| Συγγραφείς: | Stabile A; Department of Life Sciences, University of Trieste, Via L. Giorgieri 5, 34127 Trieste, Italy., Scaramella G; Department of Life Sciences, University of Trieste, Via L. Giorgieri 5, 34127 Trieste, Italy., Puccio S; Laboratory of Translational Immunology, Istituto di Ricovero e Cura a Carattere Scientifico Humanitas Research Hospital, Via Manzoni 56, 20089 Rozzano, Italy.; Institute of Genetic and Biomedical Research, National Research Council, Via Manzoni 113, 20089 Rozzano, Italy., Brady J; Food Science Department, Cornell University, 101A Stocking Hall, Ithaca, NY 14853, USA., Goltermann L; Costerton Biofilm Center, Department of Immunology and Microbiology, University of Copenhagen, Blegdamsvej 3B, DK-2200 Copenhagen, Denmark., Tolker-Nielsen T; Costerton Biofilm Center, Department of Immunology and Microbiology, University of Copenhagen, Blegdamsvej 3B, DK-2200 Copenhagen, Denmark., Bellich B; Institute for Maternal and Child Health, Istituto di Ricovero e Cura a Carattere Scientifico 'Burlo Garofolo', Via dell'Istria 65/1, 34137 Trieste, Italy., De Zotti S; Department of Life Sciences, University of Trieste, Via L. Giorgieri 5, 34127 Trieste, Italy., Lagatolla C; Department of Life Sciences, University of Trieste, Via L. Giorgieri 5, 34127 Trieste, Italy., Ferrara F; Specifica Inc., an IQVIA Laboratories Company, Santa Fe, NM 87501, USA., Rizzo R; Department of Life Sciences, University of Trieste, Via L. Giorgieri 5, 34127 Trieste, Italy., Cescutti P; Department of Life Sciences, University of Trieste, Via L. Giorgieri 5, 34127 Trieste, Italy., Sblattero D; Department of Life Sciences, University of Trieste, Via L. Giorgieri 5, 34127 Trieste, Italy. |
| Πηγή: | International journal of molecular sciences [Int J Mol Sci] 2026 Mar 06; Vol. 27 (5). Date of Electronic Publication: 2026 Mar 06. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Basel, Switzerland : MDPI, [2000- |
| Ιατρικοί όροι (MeSH): | Peptides*/metabolism , Peptides*/chemistry , Peptides*/isolation & purification , Peptides*/genetics , Peptides*/pharmacology , Burkholderia*/genetics , Burkholderia*/metabolism , Polysaccharides*/metabolism , Saccharomyces cerevisiae*/genetics , Saccharomyces cerevisiae*/metabolism , Genomic Library* , Genome, Bacterial*, Biofilms/drug effects ; Biofilms/growth & development ; Protein Binding ; Cell Surface Display Techniques ; Peptide Library |
| Περίληψη: | Here, we present a novel yeast surface display-based platform for the discovery of biofilm-associated exopolysaccharide-binding peptides. Unlike conventional synthetic libraries, our approach utilizes the genomic diversity of Burkholderia multivorans strain C1576 through open-reading frame-filtered genomic fragment libraries, thereby enriching for naturally encoded carbohydrate-binding domains. By iterative fluorescence-activated cell sorting, we identified 21 peptides with confirmed binding to two structurally distinct rhamnose-rich polysaccharides: the exopolysaccharide Epol C1576 and the capsular polysaccharide CPS KpB-1. Biophysical characterization revealed that these peptides adopt predominantly α-helical or disordered conformations and undergo structural rearrangements upon polysaccharide binding. Functional assays demonstrated that selected peptides modulate biofilm architecture and bacterial viability in a species-specific manner, although they do not have a direct bactericidal effect against planktonic cells. This proof-of-concept study establishes yeast surface display as a powerful tool for the discovery of biofilm-targeting peptides and provides a basis for development of novel diagnostics and therapeutics to combat biofilm-associated infections. |
| References: | Microbiol Mol Biol Rev. 2006 Jun;70(2):283-95. (PMID: 16760304) Antimicrob Agents Chemother. 2014 Aug;58(8):4399-403. (PMID: 24841267) Mol Biotechnol. 2025 Jun;67(6):2161-2184. (PMID: 38822912) Int J Biol Macromol. 2024 Nov;281(Pt 2):136222. (PMID: 39362422) Cell Rep Phys Sci. 2024 Mar 20;5(3):. (PMID: 38605913) Nucleic Acids Res. 2016 Jan 4;44(D1):D1087-93. (PMID: 26602694) Appl Environ Microbiol. 2013 Aug;79(15):4551-63. (PMID: 23686272) NPJ Biofilms Microbiomes. 2025 Dec 6;12(1):3. (PMID: 41353267) Biofouling. 2003 Apr;19(2):139-50. (PMID: 14618698) Biochem Biophys Res Commun. 1998 Mar 6;244(1):253-7. (PMID: 9514864) Microbiol Spectr. 2015 Jun;3(3):. (PMID: 26185074) Microbiol Spectr. 2015 Apr;3(2):. (PMID: 26104709) Anal Biochem. 2006 Jul 1;354(1):54-63. (PMID: 16713984) Nucleic Acids Res. 2014 Jan;42(Database issue):D490-5. (PMID: 24270786) Nucleic Acids Res. 2021 Jan 8;49(D1):D288-D297. (PMID: 33151284) Carbohydr Res. 2015 Jun 26;411:42-8. (PMID: 25974852) Amino Acids. 2017 Oct;49(10):1755-1771. (PMID: 28756544) Acta Crystallogr D Biol Crystallogr. 2014 Oct;70(Pt 10):2583-92. (PMID: 25286843) Genome Res. 2010 Nov;20(11):1605-12. (PMID: 20841432) J Immunol Methods. 2004 Jul;290(1-2):69-80. (PMID: 15261572) J Vis Exp. 2018 Oct 3;(140):. (PMID: 30346377) Expert Opin Drug Discov. 2024 Aug;19(8):887-915. (PMID: 39074492) Nat Commun. 2019 May 16;10(1):2183. (PMID: 31097723) Viruses. 2023 Sep 09;15(9):. (PMID: 37766309) ISME J. 2019 Jan;13(1):104-117. (PMID: 30116044) Int J Biol Macromol. 2020 Jul 15;155:315-323. (PMID: 32224183) Nucleic Acids Res. 2020 Jul 2;48(W1):W200-W207. (PMID: 32402076) Nucleic Acids Res. 2025 Jan 6;53(D1):D403-D410. (PMID: 39526377) Curr Opin Microbiol. 2016 Oct;33:35-40. (PMID: 27318321) Chem Rev. 1997 Apr 1;97(2):391-410. (PMID: 11848876) FEMS Microbiol Rev. 2022 Mar 3;46(2):. (PMID: 34673942) Antimicrob Agents Chemother. 1988 Apr;32(4):518-23. (PMID: 3132093) Nature. 2024 Jun;630(8016):493-500. (PMID: 38718835) Trends Microbiol. 2005 Jan;13(1):20-6. (PMID: 15639628) Microbiology (Reading). 2001 Jan;147(Pt 1):3-9. (PMID: 11160795) Front Immunol. 2012 Aug 14;3:249. (PMID: 22912635) Front Microbiol. 2019 May 03;10:913. (PMID: 31130925) Methods Mol Biol. 2022;2491:29-62. (PMID: 35482183) N Biotechnol. 2024 May 25;80:56-68. (PMID: 38354946) Nucleic Acids Res. 2010 May;38(9):e110. (PMID: 20144949) J Appl Microbiol. 2022 Mar;132(3):1573-1596. (PMID: 34606679) BMC Genomics. 2011 Jun 15;12 Suppl 1:S5. (PMID: 21810207) J Pept Sci. 2009 Sep;15(9):595-600. (PMID: 19466693) Appl Environ Microbiol. 2003 Mar;69(3):1408-16. (PMID: 12620823) Adv Microb Physiol. 2019;74:97-141. (PMID: 31126537) J Clin Microbiol. 2000 Feb;38(2):910-3. (PMID: 10655415) Commun Biol. 2023 Apr 5;6(1):371. (PMID: 37019943) Nucleic Acids Res. 2025 Jan 6;53(D1):D444-D456. (PMID: 39565202) Nat Commun. 2025 Jun 25;16(1):5367. (PMID: 40562762) Nucleic Acids Res. 2024 Jul 5;52(W1):W176-W181. (PMID: 38747347) |
| Grant Information: | R01 GM123283 United States GM NIGMS NIH HHS; GM123283 NIH Common Fund; NNF22OC0080132 Novo Nordisk Foundation |
| Contributed Indexing: | Keywords: antibiofilm peptides; biofilm; polysaccharides; yeast display |
| Substance Nomenclature: | 0 (Peptides) 0 (Polysaccharides) 0 (Peptide Library) |
| Entry Date(s): | Date Created: 20260314 Date Completed: 20260710 Latest Revision: 20260921 |
| Update Code: | 20260921 |
| PubMed Central ID: | PMC12986056 |
| DOI: | 10.3390/ijms27052417 |
| PMID: | 41828634 |
| Βάση Δεδομένων: | MEDLINE |
καταχωρήστε σχόλιο πρώτοι!