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Effect of solvent pretreatments on trans-dentinal cytotoxicity of bioactive materials.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Effect of solvent pretreatments on trans-dentinal cytotoxicity of bioactive materials.
Συγγραφείς: Salim IA; Department of Cariology and Restorative Dentistry, Institute of Dentistry, University of Turku.; Adhesive Dentistry Research Group, Biomaterials and Medical Device Research Program, Biocity.; Finnish Doctoral Program in Oral Sciences (FINDOS), Institute of Dentistry, University of Turku., Stape THS; Department of Cariology and Restorative Dentistry, Institute of Dentistry, University of Turku.; Adhesive Dentistry Research Group, Biomaterials and Medical Device Research Program, Biocity., Seseogullari-Dirihan R; Department of Cariology and Restorative Dentistry, Institute of Dentistry, University of Turku.; Department of General Dental Science, School of Dentistry, Marquette University., Mutluay MM; Department of Cariology and Restorative Dentistry, Institute of Dentistry, University of Turku.; Department of Oral and Maxillofacial Diseases, Institute of Dentistry, University of Helsinki., Tezvergil-Mutluay A; Department of Cariology and Restorative Dentistry, Institute of Dentistry, University of Turku.; Adhesive Dentistry Research Group, Biomaterials and Medical Device Research Program, Biocity.; Turku University Hospital, TYKS, University of Turku.
Πηγή: Dental materials journal [Dent Mater J] 2026 Mar 29; Vol. 45 (2), pp. 144-151. Date of Electronic Publication: 2026 Mar 10.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Japanese Society for Dental Materials and Devices Country of Publication: Japan NLM ID: 8309299 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1881-1361 (Electronic) Linking ISSN: 02874547 NLM ISO Abbreviation: Dent Mater J Subsets: MEDLINE
Imprint Name(s): Original Publication: Tokyo-to : Japanese Society for Dental Materials and Devices, [1982?-
Ιατρικοί όροι (MeSH): Solvents*/toxicity , Dimethyl Sulfoxide*/toxicity , Ethanol*/toxicity , Dentin*/drug effects , Biocompatible Materials*/toxicity, Cell Survival/drug effects ; Silicates/toxicity ; Oxides/toxicity ; Aluminum Compounds/toxicity ; Calcium Compounds/toxicity ; Odontoblasts/drug effects ; Dentin Permeability/drug effects ; Methacrylates/toxicity ; Humans ; Materials Testing ; Drug Combinations ; Cells, Cultured
Περίληψη: This study evaluated the effect of solvent-based dentin pretreatments containing dimethyl sulfoxide (DMSO) and/or ethanol on trans-dentinal cell viability of bioactive materials in simulated deep clinical cavities. Dentin discs (300 μm) were prepared, balanced among groups according to dentin permeability and autoclaved. Three-dimensional cultures of odontoblast-like cells were transferred to the pulpal aspect of dentin discs inside perfusion split-chambers designed for trans-dentinal cytotoxicity test following ISO 7405 standards. Dentin pretreatments were composed of ethanol (EtOH), DMSO, their 50% (v/v) aqueous dilutions and 50% (v/v) DMSO/EtOH. Pretreatments (1.5 μL) were applied on cell-seeded dentin discs and a 1-mm increment of one of three bioactive materials: a light-curable methacrylate-based silicate (TheraCal), a mineral trioxide aggregate (MTA) or an experimental pre-reacted glass-ionomer filler (S-PRG). Cell viability was analyzed spectrometrically according to the MTT test and cytotoxicity levels were classified following ISO 7405. TheraCal was considered severely cytotoxic, MTA moderately cytotoxic and S-PRG non-cytotoxic. Assessment of cell damage by solvents was ranked as EtOH=EtOH/H2O>DMSO>DMSO/EtOH=DMSO/H2O. The light-curable methacrylate-based silicate was the only material with considerable trans-dentinal cytotoxicity. While ethanol was harmful to odontoblast-like cells, DMSO had a minor role in cytotoxic responses used alone or combined to bioactive materials. Solvents must be considered for safe use during vital-pulp therapy.
Contributed Indexing: Keywords: Cell viability; DMSO; MTA; S-PRG; TheraCal
Substance Nomenclature: 0 (Solvents)
YOW8V9698H (Dimethyl Sulfoxide)
3K9958V90M (Ethanol)
0 (mineral trioxide aggregate)
0 (Drug Combinations)
0 (Silicates)
0 (Oxides)
0 (Aluminum Compounds)
0 (Calcium Compounds)
0 (Biocompatible Materials)
0 (Methacrylates)
Entry Date(s): Date Created: 20260311 Date Completed: 20260710 Latest Revision: 20260710
Update Code: 20260711
DOI: 10.4012/dmj.2025-102
PMID: 41813147
Βάση Δεδομένων: MEDLINE
FullText Text:
  Availability: 0
Header DbId: cmedm
DbLabel: MEDLINE
An: 41813147
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Effect of solvent pretreatments on trans-dentinal cytotoxicity of bioactive materials.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AU" term="%22Salim+IA%22">Salim IA</searchLink>; Department of Cariology and Restorative Dentistry, Institute of Dentistry, University of Turku.; Adhesive Dentistry Research Group, Biomaterials and Medical Device Research Program, Biocity.; Finnish Doctoral Program in Oral Sciences (FINDOS), Institute of Dentistry, University of Turku.<br /><searchLink fieldCode="AU" term="%22Stape+THS%22">Stape THS</searchLink>; Department of Cariology and Restorative Dentistry, Institute of Dentistry, University of Turku.; Adhesive Dentistry Research Group, Biomaterials and Medical Device Research Program, Biocity.<br /><searchLink fieldCode="AU" term="%22Seseogullari-Dirihan+R%22">Seseogullari-Dirihan R</searchLink>; Department of Cariology and Restorative Dentistry, Institute of Dentistry, University of Turku.; Department of General Dental Science, School of Dentistry, Marquette University.<br /><searchLink fieldCode="AU" term="%22Mutluay+MM%22">Mutluay MM</searchLink>; Department of Cariology and Restorative Dentistry, Institute of Dentistry, University of Turku.; Department of Oral and Maxillofacial Diseases, Institute of Dentistry, University of Helsinki.<br /><searchLink fieldCode="AU" term="%22Tezvergil-Mutluay+A%22">Tezvergil-Mutluay A</searchLink>; Department of Cariology and Restorative Dentistry, Institute of Dentistry, University of Turku.; Adhesive Dentistry Research Group, Biomaterials and Medical Device Research Program, Biocity.; Turku University Hospital, TYKS, University of Turku.
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%228309299%22">Dental materials journal</searchLink> [Dent Mater J] 2026 Mar 29; Vol. 45 (2), pp. 144-151. <i>Date of Electronic Publication: </i>2026 Mar 10.
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  Data: Journal Article
– Name: Language
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  Data: English
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Japanese+Society+for+Dental+Materials+and+Devices%22">Japanese Society for Dental Materials and Devices </searchLink><i>Country of Publication: </i>Japan <i>NLM ID: </i>8309299 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1881-1361 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2202874547%22">02874547 </searchLink><i>NLM ISO Abbreviation: </i>Dent Mater J <i>Subsets: </i>MEDLINE
– Name: PublisherInfo
  Label: Imprint Name(s)
  Group: PubInfo
  Data: <i>Original Publication</i>: Tokyo-to : Japanese Society for Dental Materials and Devices, [1982?-
– Name: SubjectMESH
  Label: MeSH Terms
  Group: Su
  Data: <searchLink fieldCode="MM" term="%22Solvents%22">Solvents*</searchLink>/<searchLink fieldCode="MM" term="%22Solvents+toxicity%22">toxicity</searchLink> <br /><searchLink fieldCode="MM" term="%22Dimethyl+Sulfoxide%22">Dimethyl Sulfoxide*</searchLink>/<searchLink fieldCode="MM" term="%22Dimethyl+Sulfoxide+toxicity%22">toxicity</searchLink> <br /><searchLink fieldCode="MM" term="%22Ethanol%22">Ethanol*</searchLink>/<searchLink fieldCode="MM" term="%22Ethanol+toxicity%22">toxicity</searchLink> <br /><searchLink fieldCode="MM" term="%22Dentin%22">Dentin*</searchLink>/<searchLink fieldCode="MM" term="%22Dentin+drug+effects%22">drug effects</searchLink> <br /><searchLink fieldCode="MM" term="%22Biocompatible+Materials%22">Biocompatible Materials*</searchLink>/<searchLink fieldCode="MM" term="%22Biocompatible+Materials+toxicity%22">toxicity</searchLink><br /><searchLink fieldCode="MH" term="%22Cell+Survival%22">Cell Survival</searchLink>/<searchLink fieldCode="MH" term="%22Cell+Survival+drug+effects%22">drug effects</searchLink> ; <searchLink fieldCode="MH" term="%22Silicates%22">Silicates</searchLink>/<searchLink fieldCode="MH" term="%22Silicates+toxicity%22">toxicity</searchLink> ; <searchLink fieldCode="MH" term="%22Oxides%22">Oxides</searchLink>/<searchLink fieldCode="MH" term="%22Oxides+toxicity%22">toxicity</searchLink> ; <searchLink fieldCode="MH" term="%22Aluminum+Compounds%22">Aluminum Compounds</searchLink>/<searchLink fieldCode="MH" term="%22Aluminum+Compounds+toxicity%22">toxicity</searchLink> ; <searchLink fieldCode="MH" term="%22Calcium+Compounds%22">Calcium Compounds</searchLink>/<searchLink fieldCode="MH" term="%22Calcium+Compounds+toxicity%22">toxicity</searchLink> ; <searchLink fieldCode="MH" term="%22Odontoblasts%22">Odontoblasts</searchLink>/<searchLink fieldCode="MH" term="%22Odontoblasts+drug+effects%22">drug effects</searchLink> ; <searchLink fieldCode="MH" term="%22Dentin+Permeability%22">Dentin Permeability</searchLink>/<searchLink fieldCode="MH" term="%22Dentin+Permeability+drug+effects%22">drug effects</searchLink> ; <searchLink fieldCode="MH" term="%22Methacrylates%22">Methacrylates</searchLink>/<searchLink fieldCode="MH" term="%22Methacrylates+toxicity%22">toxicity</searchLink> ; <searchLink fieldCode="MH" term="%22Humans%22">Humans</searchLink> ; <searchLink fieldCode="MH" term="%22Materials+Testing%22">Materials Testing</searchLink> ; <searchLink fieldCode="MH" term="%22Drug+Combinations%22">Drug Combinations</searchLink> ; <searchLink fieldCode="MH" term="%22Cells%2C+Cultured%22">Cells, Cultured</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: This study evaluated the effect of solvent-based dentin pretreatments containing dimethyl sulfoxide (DMSO) and/or ethanol on trans-dentinal cell viability of bioactive materials in simulated deep clinical cavities. Dentin discs (300 μm) were prepared, balanced among groups according to dentin permeability and autoclaved. Three-dimensional cultures of odontoblast-like cells were transferred to the pulpal aspect of dentin discs inside perfusion split-chambers designed for trans-dentinal cytotoxicity test following ISO 7405 standards. Dentin pretreatments were composed of ethanol (EtOH), DMSO, their 50% (v/v) aqueous dilutions and 50% (v/v) DMSO/EtOH. Pretreatments (1.5 μL) were applied on cell-seeded dentin discs and a 1-mm increment of one of three bioactive materials: a light-curable methacrylate-based silicate (TheraCal), a mineral trioxide aggregate (MTA) or an experimental pre-reacted glass-ionomer filler (S-PRG). Cell viability was analyzed spectrometrically according to the MTT test and cytotoxicity levels were classified following ISO 7405. TheraCal was considered severely cytotoxic, MTA moderately cytotoxic and S-PRG non-cytotoxic. Assessment of cell damage by solvents was ranked as EtOH=EtOH/H<subscript>2</subscript>O>DMSO>DMSO/EtOH=DMSO/H<subscript>2</subscript>O. The light-curable methacrylate-based silicate was the only material with considerable trans-dentinal cytotoxicity. While ethanol was harmful to odontoblast-like cells, DMSO had a minor role in cytotoxic responses used alone or combined to bioactive materials. Solvents must be considered for safe use during vital-pulp therapy.
– Name: SubjectMinor
  Label: Contributed Indexing
  Group:
  Data: <i>Keywords: </i>Cell viability; DMSO; MTA; S-PRG; TheraCal
– Name: NumberCAS
  Label: Substance Nomenclature
  Group: ID
  Data: 0 (Solvents)<br />YOW8V9698H (Dimethyl Sulfoxide)<br />3K9958V90M (Ethanol)<br />0 (mineral trioxide aggregate)<br />0 (Drug Combinations)<br />0 (Silicates)<br />0 (Oxides)<br />0 (Aluminum Compounds)<br />0 (Calcium Compounds)<br />0 (Biocompatible Materials)<br />0 (Methacrylates)
– Name: DateEntry
  Label: Entry Date(s)
  Group: Date
  Data: <i>Date Created: </i>20260311 <i>Date Completed: </i>20260710 <i>Latest Revision: </i>20260710
– Name: DateUpdate
  Label: Update Code
  Group: Date
  Data: 20260711
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.4012/dmj.2025-102
– Name: AN
  Label: PMID
  Group: ID
  Data: 41813147
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cmedm&AN=41813147
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.4012/dmj.2025-102
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        StartPage: 144
    Subjects:
      – SubjectFull: Cell Survival drug effects
        Type: general
      – SubjectFull: Silicates toxicity
        Type: general
      – SubjectFull: Oxides toxicity
        Type: general
      – SubjectFull: Aluminum Compounds toxicity
        Type: general
      – SubjectFull: Calcium Compounds toxicity
        Type: general
      – SubjectFull: Odontoblasts drug effects
        Type: general
      – SubjectFull: Dentin Permeability drug effects
        Type: general
      – SubjectFull: Methacrylates toxicity
        Type: general
      – SubjectFull: Humans
        Type: general
      – SubjectFull: Materials Testing
        Type: general
      – SubjectFull: Drug Combinations
        Type: general
      – SubjectFull: Cells, Cultured
        Type: general
      – SubjectFull: Solvents toxicity
        Type: general
      – SubjectFull: Dimethyl Sulfoxide toxicity
        Type: general
      – SubjectFull: Ethanol toxicity
        Type: general
      – SubjectFull: Dentin drug effects
        Type: general
      – SubjectFull: Biocompatible Materials toxicity
        Type: general
    Titles:
      – TitleFull: Effect of solvent pretreatments on trans-dentinal cytotoxicity of bioactive materials.
        Type: main
  BibRelationships:
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          Name:
            NameFull: Salim IA
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            NameFull: Stape THS
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            NameFull: Seseogullari-Dirihan R
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            NameFull: Mutluay MM
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            NameFull: Tezvergil-Mutluay A
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            – D: 29
              M: 03
              Text: 2026 Mar 29
              Type: published
              Y: 2026
          Identifiers:
            – Type: issn-electronic
              Value: 1881-1361
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              Value: 45
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              Value: 2
          Titles:
            – TitleFull: Dental materials journal
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