Effect of solvent pretreatments on trans-dentinal cytotoxicity of bioactive materials.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	Effect of solvent pretreatments on trans-dentinal cytotoxicity of bioactive materials.
Συγγραφείς:	Salim IA; Department of Cariology and Restorative Dentistry, Institute of Dentistry, University of Turku.; Adhesive Dentistry Research Group, Biomaterials and Medical Device Research Program, Biocity.; Finnish Doctoral Program in Oral Sciences (FINDOS), Institute of Dentistry, University of Turku., Stape THS; Department of Cariology and Restorative Dentistry, Institute of Dentistry, University of Turku.; Adhesive Dentistry Research Group, Biomaterials and Medical Device Research Program, Biocity., Seseogullari-Dirihan R; Department of Cariology and Restorative Dentistry, Institute of Dentistry, University of Turku.; Department of General Dental Science, School of Dentistry, Marquette University., Mutluay MM; Department of Cariology and Restorative Dentistry, Institute of Dentistry, University of Turku.; Department of Oral and Maxillofacial Diseases, Institute of Dentistry, University of Helsinki., Tezvergil-Mutluay A; Department of Cariology and Restorative Dentistry, Institute of Dentistry, University of Turku.; Adhesive Dentistry Research Group, Biomaterials and Medical Device Research Program, Biocity.; Turku University Hospital, TYKS, University of Turku.
Πηγή:	Dental materials journal [Dent Mater J] 2026 Mar 29; Vol. 45 (2), pp. 144-151. Date of Electronic Publication: 2026 Mar 10.
Τύπος έκδοσης:	Journal Article
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: Japanese Society for Dental Materials and Devices Country of Publication: Japan NLM ID: 8309299 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1881-1361 (Electronic) Linking ISSN: 02874547 NLM ISO Abbreviation: Dent Mater J Subsets: MEDLINE
Imprint Name(s):	Original Publication: Tokyo-to : Japanese Society for Dental Materials and Devices, [1982?-
Ιατρικοί όροι (MeSH):	Solvents/toxicity , Dimethyl Sulfoxide/toxicity , Ethanol/toxicity , Dentin/drug effects , Biocompatible Materials*/toxicity, Cell Survival/drug effects ; Silicates/toxicity ; Oxides/toxicity ; Aluminum Compounds/toxicity ; Calcium Compounds/toxicity ; Odontoblasts/drug effects ; Dentin Permeability/drug effects ; Methacrylates/toxicity ; Humans ; Materials Testing ; Drug Combinations ; Cells, Cultured
Περίληψη:	This study evaluated the effect of solvent-based dentin pretreatments containing dimethyl sulfoxide (DMSO) and/or ethanol on trans-dentinal cell viability of bioactive materials in simulated deep clinical cavities. Dentin discs (300 μm) were prepared, balanced among groups according to dentin permeability and autoclaved. Three-dimensional cultures of odontoblast-like cells were transferred to the pulpal aspect of dentin discs inside perfusion split-chambers designed for trans-dentinal cytotoxicity test following ISO 7405 standards. Dentin pretreatments were composed of ethanol (EtOH), DMSO, their 50% (v/v) aqueous dilutions and 50% (v/v) DMSO/EtOH. Pretreatments (1.5 μL) were applied on cell-seeded dentin discs and a 1-mm increment of one of three bioactive materials: a light-curable methacrylate-based silicate (TheraCal), a mineral trioxide aggregate (MTA) or an experimental pre-reacted glass-ionomer filler (S-PRG). Cell viability was analyzed spectrometrically according to the MTT test and cytotoxicity levels were classified following ISO 7405. TheraCal was considered severely cytotoxic, MTA moderately cytotoxic and S-PRG non-cytotoxic. Assessment of cell damage by solvents was ranked as EtOH=EtOH/H2O>DMSO>DMSO/EtOH=DMSO/H2O. The light-curable methacrylate-based silicate was the only material with considerable trans-dentinal cytotoxicity. While ethanol was harmful to odontoblast-like cells, DMSO had a minor role in cytotoxic responses used alone or combined to bioactive materials. Solvents must be considered for safe use during vital-pulp therapy.
Contributed Indexing:	Keywords: Cell viability; DMSO; MTA; S-PRG; TheraCal
Substance Nomenclature:	0 (Solvents) YOW8V9698H (Dimethyl Sulfoxide) 3K9958V90M (Ethanol) 0 (mineral trioxide aggregate) 0 (Drug Combinations) 0 (Silicates) 0 (Oxides) 0 (Aluminum Compounds) 0 (Calcium Compounds) 0 (Biocompatible Materials) 0 (Methacrylates)
Entry Date(s):	Date Created: 20260311 Date Completed: 20260710 Latest Revision: 20260710
Update Code:	20260711
DOI:	10.4012/dmj.2025-102
PMID:	41813147
Βάση Δεδομένων:	MEDLINE

Περιγραφή
ISSN:	1881-1361
DOI:	10.4012/dmj.2025-102