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Patient-Derived IgG Epitope Mapping of Bet v 1 Reveals Hypoallergenic Peptide Candidates for Safe and Next-Generation Allergen Immunotherapy.

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Τίτλος: Patient-Derived IgG Epitope Mapping of Bet v 1 Reveals Hypoallergenic Peptide Candidates for Safe and Next-Generation Allergen Immunotherapy.
Συγγραφείς: Šošić L; Department of Dermatology, University of Zurich, Schlieren, Switzerland., Paolucci M; Department of Dermatology, University of Zurich, Schlieren, Switzerland., Streuli A; Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland.; Laboratory for Pharmaceutical Analytics, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland., Melillo DA; Department of Dermatology, University of Zurich, Schlieren, Switzerland., Jiang T; Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria., Campana R; Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria., Lang CCV; Department of Dermatology, University of Zurich, Schlieren, Switzerland.; Department of Dermatology, University Hospital Zurich, Zurich, Switzerland., Kündig TM; Department of Dermatology, University of Zurich, Schlieren, Switzerland.; Department of Dermatology, University Hospital Zurich, Zurich, Switzerland., Steuer C; Laboratory for Pharmaceutical Analytics, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland., Eyer K; Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland.; Department of Biomedicine, Aarhus University, Aarhus, Denmark., Johansen P; Department of Dermatology, University of Zurich, Schlieren, Switzerland.; Department of Dermatology, University Hospital Zurich, Zurich, Switzerland.
Πηγή: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology [Clin Exp Allergy] 2026 Aug; Vol. 56 (8), pp. 881-892. Date of Electronic Publication: 2026 Mar 10.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 8906443 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2222 (Electronic) Linking ISSN: 09547894 NLM ISO Abbreviation: Clin Exp Allergy Subsets: MEDLINE
Imprint Name(s): Original Publication: Oxford : Blackwell Scientific Publications, c1989-
Ιατρικοί όροι (MeSH): Immunoglobulin G*/immunology , Immunoglobulin G*/blood , Desensitization, Immunologic*/methods , Antigens, Plant*/immunology , Antigens, Plant*/chemistry , Allergens*/immunology , Peptides*/immunology , Rhinitis, Allergic, Seasonal*/immunology , Rhinitis, Allergic, Seasonal*/therapy , Epitope Mapping*, Basophils/immunology ; Immunoglobulin E/immunology ; Immunoglobulin E/blood ; Epitopes/immunology ; Humans ; Female ; Adult ; Male
Περίληψη: Background: Allergen immunotherapy (AIT) is the only curative treatment for allergic diseases, primarily by inducing allergen-neutralising IgG antibodies. However, its use is limited by frequent allergic adverse events.
Objective: To facilitate the design of IgG-epitope-based peptide vaccines, this study aimed to identify IgG-binding regions on the major birch pollen allergen, Bet v 1, using patient-derived immune profiles.
Methods: Blood samples were collected from 30 birch pollen-allergic patients, both AIT-treated and untreated; healthy individuals were included as controls. Sera were analysed for allergen-specific IgE, IgG, and IgG4, and basophil degranulation inhibition was assessed in humanised RBL cells and blood from allergic individuals. We used DropMap microfluidics for single-cell affinity profiling of IgG- and IgG4-secreting B cells. IgG epitopes were mapped using overlapping peptides and CLIPS technology. Candidate AIT peptides were synthesised on solid phase via Fmoc strategy, and their allergenicity was assessed in basophil degranulation and cellular antigen stimulation assays.
Results: Sera from AIT-treated patients exhibited elevated IgG and IgG4 levels and enhanced inhibition of basophil degranulation. Single-cell analysis indicated IgG-affinity maturation with AIT. IgG-epitope mapping identified four distinct, non-overlapping IgG-binding regions on Bet v 1. Six peptides derived from these regions were successfully produced and did not induce basophil degranulation in vitro. The application of DropMap microfluidics allowed high-resolution single B-cell analysis, providing novel insights into allergen-specific B-cell responses and the effects of AIT at the clonal level.
Conclusion: This integrated approach identifies hypoallergenic peptides that selectively engage protective IgG responses and offers a framework for developing safer, next-generation immunotherapies for allergic disease.
(© 2026 The Author(s). Clinical & Experimental Allergy published by John Wiley & Sons Ltd.)
References: Nat Rev Immunol. 2006 Oct;6(10):761-71. (PMID: 16998509)
Allergy. 2013 Nov;68(11):1377-86. (PMID: 24053565)
Int J Cancer. 2011 Jul 15;129(2):397-406. (PMID: 21207427)
Am J Respir Crit Care Med. 2021 Jul 1;204(1):23-33. (PMID: 33651675)
J Allergy Clin Immunol. 2015 Dec;136(6):1680-1682.e3. (PMID: 26143181)
Clin Exp Allergy. 2021 Jun;51(6):751-769. (PMID: 33529435)
J Immunol. 2003 Sep 15;171(6):3084-90. (PMID: 12960334)
FEBS Lett. 2000 Jan 7;465(1):39-46. (PMID: 10620703)
J Allergy Clin Immunol. 2022 May;149(5):1786-1794.e12. (PMID: 34740603)
NPJ Vaccines. 2021 Jan 18;6(1):12. (PMID: 33462231)
J Immunol. 1996 Jul 15;157(2):956-62. (PMID: 8752951)
J Allergy Clin Immunol. 2023 May;151(5):1371-1378.e5. (PMID: 36657603)
Allergy. 1998;53(48 Suppl):60-3. (PMID: 10096811)
J Allergy Clin Immunol. 2013 Jan;131(1):128-34.e1-3. (PMID: 23199605)
J Immunol. 2011 May 1;186(9):5333-44. (PMID: 21451110)
J Allergy Clin Immunol. 2022 Jan;149(1):200-211. (PMID: 34126155)
Nat Biotechnol. 2017 Oct;35(10):977-982. (PMID: 28892076)
J Allergy Clin Immunol. 2011 Feb;127(2):509-516.e1-5. (PMID: 21281875)
Allergy. 2020 May;75(5):1121-1132. (PMID: 31587307)
Nat Commun. 2018 Apr 12;9(1):1421. (PMID: 29650949)
Allergy. 2023 Dec;78(12):3136-3153. (PMID: 37701941)
Lancet Reg Health Eur. 2021 Nov 30;13:100275. (PMID: 34901915)
J Allergy Clin Immunol. 2022 Jan;149(1):189-199. (PMID: 34126156)
J Immunol. 2013 Apr 1;190(7):3068-78. (PMID: 23440415)
J Allergy Clin Immunol. 2019 Mar;143(3):1258-1261. (PMID: 30471304)
Clin Exp Allergy. 2017 May;47(5):693-703. (PMID: 27770477)
Curr Treat Options Allergy. 2014 Feb 26;1:91-106. (PMID: 24860720)
FASEB J. 1998 Feb;12(2):231-42. (PMID: 9472988)
Clin Exp Allergy. 2004 Oct;34(10):1525-33. (PMID: 15479266)
Vaccine. 2001 Sep 14;19(32):4652-60. (PMID: 11535313)
Rev Med Virol. 2019 May;29(3):e2034. (PMID: 30706584)
Expert Rev Clin Immunol. 2023 Jul-Dec;19(7):745-769. (PMID: 37122076)
Allergy. 2022 Aug;77(8):2446-2458. (PMID: 35403221)
J Allergy Clin Immunol. 2011 Mar;127(3):608-15.e1-5. (PMID: 21093025)
J Allergy Clin Immunol. 2022 Mar;149(3):791-801. (PMID: 35093483)
Clin Transl Allergy. 2023 May;13(5):e12245. (PMID: 37227419)
Insect Biochem Mol Biol. 2019 Feb;105:10-24. (PMID: 30582958)
J Immunol. 2000 Jul 1;165(1):331-8. (PMID: 10861069)
Grant Information: 25866.2 PFLS-LS Innosuisse - Schweizerische Agentur für Innovationsförderung; 407940_206399 Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung; STEB-2022-002 Foundation for Research in Science and the Humanities at the University of Zurich
Contributed Indexing: Keywords: DropMap; allergen immunotherapy; antibodies; basophil activation assay; epitope mapping; synthetic peptides
Substance Nomenclature: 0 (Immunoglobulin G)
0 (Antigens, Plant)
0 (Allergens)
0 (Peptides)
126161-14-6 (Bet v 1 allergen, Betula)
37341-29-0 (Immunoglobulin E)
0 (Epitopes)
Entry Date(s): Date Created: 20260310 Date Completed: 20260802 Latest Revision: 20260813
Update Code: 20260813
PubMed Central ID: PMC13429318
DOI: 10.1111/cea.70276
PMID: 41806123
Βάση Δεδομένων: MEDLINE
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  Data: Patient-Derived IgG Epitope Mapping of Bet v 1 Reveals Hypoallergenic Peptide Candidates for Safe and Next-Generation Allergen Immunotherapy.
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  Data: <searchLink fieldCode="AU" term="%22Šošić+L%22">Šošić L</searchLink>; Department of Dermatology, University of Zurich, Schlieren, Switzerland.<br /><searchLink fieldCode="AU" term="%22Paolucci+M%22">Paolucci M</searchLink>; Department of Dermatology, University of Zurich, Schlieren, Switzerland.<br /><searchLink fieldCode="AU" term="%22Streuli+A%22">Streuli A</searchLink>; Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland.; Laboratory for Pharmaceutical Analytics, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland.<br /><searchLink fieldCode="AU" term="%22Melillo+DA%22">Melillo DA</searchLink>; Department of Dermatology, University of Zurich, Schlieren, Switzerland.<br /><searchLink fieldCode="AU" term="%22Jiang+T%22">Jiang T</searchLink>; Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.<br /><searchLink fieldCode="AU" term="%22Campana+R%22">Campana R</searchLink>; Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.<br /><searchLink fieldCode="AU" term="%22Lang+CCV%22">Lang CCV</searchLink>; Department of Dermatology, University of Zurich, Schlieren, Switzerland.; Department of Dermatology, University Hospital Zurich, Zurich, Switzerland.<br /><searchLink fieldCode="AU" term="%22Kündig+TM%22">Kündig TM</searchLink>; Department of Dermatology, University of Zurich, Schlieren, Switzerland.; Department of Dermatology, University Hospital Zurich, Zurich, Switzerland.<br /><searchLink fieldCode="AU" term="%22Steuer+C%22">Steuer C</searchLink>; Laboratory for Pharmaceutical Analytics, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland.<br /><searchLink fieldCode="AU" term="%22Eyer+K%22">Eyer K</searchLink>; Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland.; Department of Biomedicine, Aarhus University, Aarhus, Denmark.<br /><searchLink fieldCode="AU" term="%22Johansen+P%22">Johansen P</searchLink>; Department of Dermatology, University of Zurich, Schlieren, Switzerland.; Department of Dermatology, University Hospital Zurich, Zurich, Switzerland.
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  Data: <searchLink fieldCode="JN" term="%228906443%22">Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology</searchLink> [Clin Exp Allergy] 2026 Aug; Vol. 56 (8), pp. 881-892. <i>Date of Electronic Publication: </i>2026 Mar 10.
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Blackwell+Scientific+Publications%22">Blackwell Scientific Publications </searchLink><i>Country of Publication: </i>England <i>NLM ID: </i>8906443 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1365-2222 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2209547894%22">09547894 </searchLink><i>NLM ISO Abbreviation: </i>Clin Exp Allergy <i>Subsets: </i>MEDLINE
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  Data: <i>Original Publication</i>: Oxford : Blackwell Scientific Publications, c1989-
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  Data: <searchLink fieldCode="MM" term="%22Immunoglobulin+G%22">Immunoglobulin G*</searchLink>/<searchLink fieldCode="MM" term="%22Immunoglobulin+G+immunology%22">immunology</searchLink> <br /><searchLink fieldCode="MM" term="%22Immunoglobulin+G%22">Immunoglobulin G*</searchLink>/<searchLink fieldCode="MM" term="%22Immunoglobulin+G+blood%22">blood</searchLink> <br /><searchLink fieldCode="MM" term="%22Desensitization%2C+Immunologic%22">Desensitization, Immunologic*</searchLink>/<searchLink fieldCode="MM" term="%22Desensitization%2C+Immunologic+methods%22">methods</searchLink> <br /><searchLink fieldCode="MM" term="%22Antigens%2C+Plant%22">Antigens, Plant*</searchLink>/<searchLink fieldCode="MM" term="%22Antigens%2C+Plant+immunology%22">immunology</searchLink> <br /><searchLink fieldCode="MM" term="%22Antigens%2C+Plant%22">Antigens, Plant*</searchLink>/<searchLink fieldCode="MM" term="%22Antigens%2C+Plant+chemistry%22">chemistry</searchLink> <br /><searchLink fieldCode="MM" term="%22Allergens%22">Allergens*</searchLink>/<searchLink fieldCode="MM" term="%22Allergens+immunology%22">immunology</searchLink> <br /><searchLink fieldCode="MM" term="%22Peptides%22">Peptides*</searchLink>/<searchLink fieldCode="MM" term="%22Peptides+immunology%22">immunology</searchLink> <br /><searchLink fieldCode="MM" term="%22Rhinitis%2C+Allergic%2C+Seasonal%22">Rhinitis, Allergic, Seasonal*</searchLink>/<searchLink fieldCode="MM" term="%22Rhinitis%2C+Allergic%2C+Seasonal+immunology%22">immunology</searchLink> <br /><searchLink fieldCode="MM" term="%22Rhinitis%2C+Allergic%2C+Seasonal%22">Rhinitis, Allergic, Seasonal*</searchLink>/<searchLink fieldCode="MM" term="%22Rhinitis%2C+Allergic%2C+Seasonal+therapy%22">therapy</searchLink> <br /><searchLink fieldCode="MM" term="%22Epitope+Mapping%22">Epitope Mapping*</searchLink><br /><searchLink fieldCode="MH" term="%22Basophils%22">Basophils</searchLink>/<searchLink fieldCode="MH" term="%22Basophils+immunology%22">immunology</searchLink> ; <searchLink fieldCode="MH" term="%22Immunoglobulin+E%22">Immunoglobulin E</searchLink>/<searchLink fieldCode="MH" term="%22Immunoglobulin+E+immunology%22">immunology</searchLink> ; <searchLink fieldCode="MH" term="%22Immunoglobulin+E%22">Immunoglobulin E</searchLink>/<searchLink fieldCode="MH" term="%22Immunoglobulin+E+blood%22">blood</searchLink> ; <searchLink fieldCode="MH" term="%22Epitopes%22">Epitopes</searchLink>/<searchLink fieldCode="MH" term="%22Epitopes+immunology%22">immunology</searchLink> ; <searchLink fieldCode="MH" term="%22Humans%22">Humans</searchLink> ; <searchLink fieldCode="MH" term="%22Female%22">Female</searchLink> ; <searchLink fieldCode="MH" term="%22Adult%22">Adult</searchLink> ; <searchLink fieldCode="MH" term="%22Male%22">Male</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Background: Allergen immunotherapy (AIT) is the only curative treatment for allergic diseases, primarily by inducing allergen-neutralising IgG antibodies. However, its use is limited by frequent allergic adverse events.<br />Objective: To facilitate the design of IgG-epitope-based peptide vaccines, this study aimed to identify IgG-binding regions on the major birch pollen allergen, Bet v 1, using patient-derived immune profiles.<br />Methods: Blood samples were collected from 30 birch pollen-allergic patients, both AIT-treated and untreated; healthy individuals were included as controls. Sera were analysed for allergen-specific IgE, IgG, and IgG4, and basophil degranulation inhibition was assessed in humanised RBL cells and blood from allergic individuals. We used DropMap microfluidics for single-cell affinity profiling of IgG- and IgG4-secreting B cells. IgG epitopes were mapped using overlapping peptides and CLIPS technology. Candidate AIT peptides were synthesised on solid phase via Fmoc strategy, and their allergenicity was assessed in basophil degranulation and cellular antigen stimulation assays.<br />Results: Sera from AIT-treated patients exhibited elevated IgG and IgG4 levels and enhanced inhibition of basophil degranulation. Single-cell analysis indicated IgG-affinity maturation with AIT. IgG-epitope mapping identified four distinct, non-overlapping IgG-binding regions on Bet v 1. Six peptides derived from these regions were successfully produced and did not induce basophil degranulation in vitro. The application of DropMap microfluidics allowed high-resolution single B-cell analysis, providing novel insights into allergen-specific B-cell responses and the effects of AIT at the clonal level.<br />Conclusion: This integrated approach identifies hypoallergenic peptides that selectively engage protective IgG responses and offers a framework for developing safer, next-generation immunotherapies for allergic disease.<br /> (© 2026 The Author(s). Clinical & Experimental Allergy published by John Wiley & Sons Ltd.)
– Name: Ref
  Label: References
  Group: RefInfo
  Data: Nat Rev Immunol. 2006 Oct;6(10):761-71. (PMID: <searchLink fieldCode="PM" term="%2216998509%22">16998509)</searchLink><br />Allergy. 2013 Nov;68(11):1377-86. (PMID: <searchLink fieldCode="PM" term="%2224053565%22">24053565)</searchLink><br />Int J Cancer. 2011 Jul 15;129(2):397-406. (PMID: <searchLink fieldCode="PM" term="%2221207427%22">21207427)</searchLink><br />Am J Respir Crit Care Med. 2021 Jul 1;204(1):23-33. (PMID: <searchLink fieldCode="PM" term="%2233651675%22">33651675)</searchLink><br />J Allergy Clin Immunol. 2015 Dec;136(6):1680-1682.e3. (PMID: <searchLink fieldCode="PM" term="%2226143181%22">26143181)</searchLink><br />Clin Exp Allergy. 2021 Jun;51(6):751-769. (PMID: <searchLink fieldCode="PM" term="%2233529435%22">33529435)</searchLink><br />J Immunol. 2003 Sep 15;171(6):3084-90. (PMID: <searchLink fieldCode="PM" term="%2212960334%22">12960334)</searchLink><br />FEBS Lett. 2000 Jan 7;465(1):39-46. (PMID: <searchLink fieldCode="PM" term="%2210620703%22">10620703)</searchLink><br />J Allergy Clin Immunol. 2022 May;149(5):1786-1794.e12. (PMID: <searchLink fieldCode="PM" term="%2234740603%22">34740603)</searchLink><br />NPJ Vaccines. 2021 Jan 18;6(1):12. (PMID: <searchLink fieldCode="PM" term="%2233462231%22">33462231)</searchLink><br />J Immunol. 1996 Jul 15;157(2):956-62. (PMID: <searchLink fieldCode="PM" term="%228752951%22">8752951)</searchLink><br />J Allergy Clin Immunol. 2023 May;151(5):1371-1378.e5. (PMID: <searchLink fieldCode="PM" term="%2236657603%22">36657603)</searchLink><br />Allergy. 1998;53(48 Suppl):60-3. (PMID: <searchLink fieldCode="PM" term="%2210096811%22">10096811)</searchLink><br />J Allergy Clin Immunol. 2013 Jan;131(1):128-34.e1-3. (PMID: <searchLink fieldCode="PM" term="%2223199605%22">23199605)</searchLink><br />J Immunol. 2011 May 1;186(9):5333-44. (PMID: <searchLink fieldCode="PM" term="%2221451110%22">21451110)</searchLink><br />J Allergy Clin Immunol. 2022 Jan;149(1):200-211. (PMID: <searchLink fieldCode="PM" term="%2234126155%22">34126155)</searchLink><br />Nat Biotechnol. 2017 Oct;35(10):977-982. (PMID: <searchLink fieldCode="PM" term="%2228892076%22">28892076)</searchLink><br />J Allergy Clin Immunol. 2011 Feb;127(2):509-516.e1-5. (PMID: <searchLink fieldCode="PM" term="%2221281875%22">21281875)</searchLink><br />Allergy. 2020 May;75(5):1121-1132. (PMID: <searchLink fieldCode="PM" term="%2231587307%22">31587307)</searchLink><br />Nat Commun. 2018 Apr 12;9(1):1421. (PMID: <searchLink fieldCode="PM" term="%2229650949%22">29650949)</searchLink><br />Allergy. 2023 Dec;78(12):3136-3153. (PMID: <searchLink fieldCode="PM" term="%2237701941%22">37701941)</searchLink><br />Lancet Reg Health Eur. 2021 Nov 30;13:100275. (PMID: <searchLink fieldCode="PM" term="%2234901915%22">34901915)</searchLink><br />J Allergy Clin Immunol. 2022 Jan;149(1):189-199. (PMID: <searchLink fieldCode="PM" term="%2234126156%22">34126156)</searchLink><br />J Immunol. 2013 Apr 1;190(7):3068-78. (PMID: <searchLink fieldCode="PM" term="%2223440415%22">23440415)</searchLink><br />J Allergy Clin Immunol. 2019 Mar;143(3):1258-1261. (PMID: <searchLink fieldCode="PM" term="%2230471304%22">30471304)</searchLink><br />Clin Exp Allergy. 2017 May;47(5):693-703. (PMID: <searchLink fieldCode="PM" term="%2227770477%22">27770477)</searchLink><br />Curr Treat Options Allergy. 2014 Feb 26;1:91-106. (PMID: <searchLink fieldCode="PM" term="%2224860720%22">24860720)</searchLink><br />FASEB J. 1998 Feb;12(2):231-42. (PMID: <searchLink fieldCode="PM" term="%229472988%22">9472988)</searchLink><br />Clin Exp Allergy. 2004 Oct;34(10):1525-33. (PMID: <searchLink fieldCode="PM" term="%2215479266%22">15479266)</searchLink><br />Vaccine. 2001 Sep 14;19(32):4652-60. (PMID: <searchLink fieldCode="PM" term="%2211535313%22">11535313)</searchLink><br />Rev Med Virol. 2019 May;29(3):e2034. (PMID: <searchLink fieldCode="PM" term="%2230706584%22">30706584)</searchLink><br />Expert Rev Clin Immunol. 2023 Jul-Dec;19(7):745-769. (PMID: <searchLink fieldCode="PM" term="%2237122076%22">37122076)</searchLink><br />Allergy. 2022 Aug;77(8):2446-2458. (PMID: <searchLink fieldCode="PM" term="%2235403221%22">35403221)</searchLink><br />J Allergy Clin Immunol. 2011 Mar;127(3):608-15.e1-5. (PMID: <searchLink fieldCode="PM" term="%2221093025%22">21093025)</searchLink><br />J Allergy Clin Immunol. 2022 Mar;149(3):791-801. (PMID: <searchLink fieldCode="PM" term="%2235093483%22">35093483)</searchLink><br />Clin Transl Allergy. 2023 May;13(5):e12245. (PMID: <searchLink fieldCode="PM" term="%2237227419%22">37227419)</searchLink><br />Insect Biochem Mol Biol. 2019 Feb;105:10-24. (PMID: <searchLink fieldCode="PM" term="%2230582958%22">30582958)</searchLink><br />J Immunol. 2000 Jul 1;165(1):331-8. (PMID: <searchLink fieldCode="PM" term="%2210861069%22">10861069)</searchLink>
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  Data: 25866.2 PFLS-LS Innosuisse - Schweizerische Agentur für Innovationsförderung; 407940_206399 Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung; STEB-2022-002 Foundation for Research in Science and the Humanities at the University of Zurich
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  Data: <i>Keywords: </i>DropMap; allergen immunotherapy; antibodies; basophil activation assay; epitope mapping; synthetic peptides
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  Data: 0 (Immunoglobulin G)<br />0 (Antigens, Plant)<br />0 (Allergens)<br />0 (Peptides)<br />126161-14-6 (Bet v 1 allergen, Betula)<br />37341-29-0 (Immunoglobulin E)<br />0 (Epitopes)
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        StartPage: 881
    Subjects:
      – SubjectFull: Basophils immunology
        Type: general
      – SubjectFull: Immunoglobulin E immunology
        Type: general
      – SubjectFull: Immunoglobulin E blood
        Type: general
      – SubjectFull: Epitopes immunology
        Type: general
      – SubjectFull: Humans
        Type: general
      – SubjectFull: Female
        Type: general
      – SubjectFull: Adult
        Type: general
      – SubjectFull: Male
        Type: general
      – SubjectFull: Immunoglobulin G immunology
        Type: general
      – SubjectFull: Immunoglobulin G blood
        Type: general
      – SubjectFull: Desensitization, Immunologic methods
        Type: general
      – SubjectFull: Antigens, Plant immunology
        Type: general
      – SubjectFull: Antigens, Plant chemistry
        Type: general
      – SubjectFull: Allergens immunology
        Type: general
      – SubjectFull: Peptides immunology
        Type: general
      – SubjectFull: Rhinitis, Allergic, Seasonal immunology
        Type: general
      – SubjectFull: Rhinitis, Allergic, Seasonal therapy
        Type: general
      – SubjectFull: Epitope Mapping
        Type: general
    Titles:
      – TitleFull: Patient-Derived IgG Epitope Mapping of Bet v 1 Reveals Hypoallergenic Peptide Candidates for Safe and Next-Generation Allergen Immunotherapy.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Šošić L
      – PersonEntity:
          Name:
            NameFull: Paolucci M
      – PersonEntity:
          Name:
            NameFull: Streuli A
      – PersonEntity:
          Name:
            NameFull: Melillo DA
      – PersonEntity:
          Name:
            NameFull: Jiang T
      – PersonEntity:
          Name:
            NameFull: Campana R
      – PersonEntity:
          Name:
            NameFull: Lang CCV
      – PersonEntity:
          Name:
            NameFull: Kündig TM
      – PersonEntity:
          Name:
            NameFull: Steuer C
      – PersonEntity:
          Name:
            NameFull: Eyer K
      – PersonEntity:
          Name:
            NameFull: Johansen P
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 08
              Text: 2026 Aug
              Type: published
              Y: 2026
          Identifiers:
            – Type: issn-electronic
              Value: 1365-2222
          Numbering:
            – Type: volume
              Value: 56
            – Type: issue
              Value: 8
          Titles:
            – TitleFull: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
              Type: main
ResultId 1