Academic Journal
Field-deployable LAMP assay for rapid and specific detection of Fusarium oxysporum causing corm rot in saffron.
| Τίτλος: | Field-deployable LAMP assay for rapid and specific detection of Fusarium oxysporum causing corm rot in saffron. |
|---|---|
| Συγγραφείς: | Tripathi V; Division of Plant Pathology, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Chatha, 180009, India. Electronic address: vansht255@gmail.com., Gupta V; Division of Plant Pathology, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Chatha, 180009, India. Electronic address: vishalgupta16@skuastj.org., Gupta SK; Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Chatha, 180009, India. Electronic address: sushildr@skuastj.org., Amin Z; ICAR-Central Institute of Temperate Horticulture, Srinagar, 191132, India. Electronic address: zakiraminmalik@skuastkashmir.ac.in., Ashraf S; Department of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore, 641003, India. Electronic address: suhailashraf9906@gmail.com., Al Tami MS; Department of Biology, College of Science, Qassim University, PO Box 6622, Buraidah, 51452, Qassim, Saudi Arabia. Electronic address: Tamie@qu.edu.sa., Hamed KE; Department of Plant Protection, College of Agriculture and Food, Qassim University, PO Box 6622, Buraidah, 51452, Qassim, Saudi Arabia. Electronic address: kh.mohammed@qu.edu.sa., Pandit V; Centre for Agriculture and Bioscience International (CABI), New Delhi, 110 012, India. Electronic address: v.pandit@cabi.org., Gupta G; Division of Plant Breeding & Genetics, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Chatha, 180009, India. |
| Πηγή: | Talanta [Talanta] 2026 Jul 01; Vol. 304, pp. 129567. Date of Electronic Publication: 2026 Feb 18. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: MEDLINE |
| Imprint Name(s): | Publication: Amsterdam : Elsevier Original Publication: Oxford : Pergamon Press |
| Ιατρικοί όροι (MeSH): | Fusarium*/isolation & purification , Fusarium*/genetics , Crocus*/microbiology , Nucleic Acid Amplification Techniques*/methods , Plant Diseases*/microbiology , Molecular Diagnostic Techniques*/methods, DNA Primers ; Rapid Diagnostic Tests ; Polymerase Chain Reaction |
| Περίληψη: | A field-deployable and highly specific loop-mediated isothermal amplification (LAMP) assay was developed and validated for the rapid detection of Fusarium oxysporum, the causal agent of corm rot in saffron. Corm rot poses a serious threat to saffron (Crocus sativus L.) cultivation, especially during the flowering stage, resulting in significant yield losses. This study focused on the molecular identification and rapid detection of F. oxysporum in saffron-growing regions of Kishtwar, Jammu and Kashmir. Cultures of F. oxysporum and eleven other microbial isolates were obtained, and molecular identification was conducted via Sanger sequencing using universal ITS1-F and ITS4-R primers for fungi and 27F/1492R primers for bacteria. Complete genome sequences of all isolates were retrieved from NCBI for comparative analysis. A unique 500 bp sequence specific to F. oxysporum, located between positions 5,500-6,000 on chromosome XII, was identified for designing specific PCR and LAMP primers, which were validated both in silico and experimentally (gel electrophoresis and SYBR Green dye). The LAMP detection method proved simple, sensitive, and suitable for early, on-site detection, as it yields positive results exclusively for F. oxysporum. Sensitivity analysis showed detection as low as 2 ng/μL of DNA, suitable for early on-site diagnosis. The operational simplicity requiring only a basic heat source and straightforward result interpretation makes it suitable for field use in resource-limited settings. Early diagnosis enables timely interventions to limit disease spread and crop losses, while the adaptability of the assay supports broader use in plant health monitoring and crop disease management. (Copyright © 2026 Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Corm rot; Fusarium oxysporum; Loop-mediated isothermal amplification (LAMP); Molecular diagnosis; Plant pathogen detection saffron (Crocus sativus) |
| Substance Nomenclature: | 0 (DNA Primers) |
| SCR Protocol: | LAMP assay |
| SCR Organism: | Fusarium oxysporum |
| Entry Date(s): | Date Created: 20260222 Date Completed: 20260708 Latest Revision: 20260708 |
| Update Code: | 20260708 |
| DOI: | 10.1016/j.talanta.2026.129567 |
| PMID: | 41724110 |
| Βάση Δεδομένων: | MEDLINE |
καταχωρήστε σχόλιο πρώτοι!