Academic Journal
20-hydroxyecdysone regulated exosomal transport of mulberry urease drives nitrogen recycling and silkworm development.
| Τίτλος: | 20-hydroxyecdysone regulated exosomal transport of mulberry urease drives nitrogen recycling and silkworm development. |
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| Συγγραφείς: | Li M; State Key Laboratory of Resource Insects, Institute of Sericulture and Systems Biology, Southwest University, Chongqing400715, People's Republic of China., Liang J; State Key Laboratory of Resource Insects, Institute of Sericulture and Systems Biology, Southwest University, Chongqing400715, People's Republic of China., Lu H; State Key Laboratory of Resource Insects, Institute of Sericulture and Systems Biology, Southwest University, Chongqing400715, People's Republic of China., Liu H; State Key Laboratory of Resource Insects, Institute of Sericulture and Systems Biology, Southwest University, Chongqing400715, People's Republic of China., Chen L; State Key Laboratory of Resource Insects, Institute of Sericulture and Systems Biology, Southwest University, Chongqing400715, People's Republic of China., Chen K; State Key Laboratory of Resource Insects, Institute of Sericulture and Systems Biology, Southwest University, Chongqing400715, People's Republic of China., Zhang S; State Key Laboratory of Resource Insects, Institute of Sericulture and Systems Biology, Southwest University, Chongqing400715, People's Republic of China., He N; State Key Laboratory of Resource Insects, Institute of Sericulture and Systems Biology, Southwest University, Chongqing400715, People's Republic of China. |
| Πηγή: | Open biology [Open Biol] 2026 Feb 18; Vol. 16 (2). |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Royal Society Pub Country of Publication: England NLM ID: 101580419 Publication Model: Print Cited Medium: Internet ISSN: 2046-2441 (Electronic) Linking ISSN: 20462441 NLM ISO Abbreviation: Open Biol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: London : Royal Society Pub., [2011]- |
| Ιατρικοί όροι (MeSH): | Bombyx*/growth & development , Bombyx*/metabolism , Morus*/enzymology , Ecdysterone*/metabolism , Ecdysterone*/pharmacology , Urease*/metabolism , Nitrogen*/metabolism , Exosomes*/metabolism, Hemolymph/metabolism ; Larva/growth & development ; Larva/metabolism ; Animals ; Biological Transport |
| Περίληψη: | The recycling of urea nitrogenous by urease presents a valuable approach for optimizing nitrogen utilization in organisms with limited nitrogen resources. In silkworms, haemolymph urea concentration correlates with urease activity, and the urease is derived from their exclusive food source, mulberry leaves. Although mulberry-derived urease has been shown to cross the silkworm midgut barrier, the mechanism of its translocation into the haemolymph remains unknown. In the present study, we investigated the transport mechanism of mulberry urease into the silkworm haemolymph using Western blotting and immunoelectron microscopy. Our results revealed that exosomes and autophagy-related factors, which are regulated by 20-hydroxyecdysone (20E), mediate the stage-specific transport of mulberry urease from the midgut to the haemolymph in silkworm larvae. Urease deficiency impaired silkworm growth and development, significantly reducing cocoon shell weight and egg production. These findings highlight the profound influence of host plants on insect phenotypic traits and reveal a novel cross-kingdom molecular transport mechanism. Our results not only deepen the understanding of plant-insect interactions but also offer practical insights for sericulture improvement, particularly in formulating artificial diets to enhance silk production. (© 2026 The Authors.) |
| Grant Information: | National Key Research and Development Program of China; Science and Technology Pilot Project-Key Research and Development Project |
| Contributed Indexing: | Keywords: 20-hydroxyecdysone; exosome; mulberry urease; pest management; silkworm |
| Substance Nomenclature: | 5289-74-7 (Ecdysterone) EC 3.5.1.5 (Urease) N762921K75 (Nitrogen) |
| Entry Date(s): | Date Created: 20260217 Date Completed: 20260707 Latest Revision: 20260707 |
| Update Code: | 20260708 |
| DOI: | 10.1098/rsob.250231 |
| PMID: | 41702423 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 2046-2441 |
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| DOI: | 10.1098/rsob.250231 |