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Genetic Diversity and Population Structure of Tunisian Festuca Arundinacea Schreb. Revealed by Functional Gene-Based Markers.

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Τίτλος: Genetic Diversity and Population Structure of Tunisian Festuca Arundinacea Schreb. Revealed by Functional Gene-Based Markers.
Συγγραφείς: Chadded H; Faculté des Sciences de Tunis, Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie (LR99ES12), Université de Tunis El Manar, Campus Universitaire Farhat Hached, 2092, El Manar, Tunis, Tunisia., Guenni K; Faculté des Sciences de Tunis, Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie (LR99ES12), Université de Tunis El Manar, Campus Universitaire Farhat Hached, 2092, El Manar, Tunis, Tunisia., Crespan M; CREA Research Centre for Viticulture and Enology, Viale XXVIII Aprile, 26, 31015, Conegliano, Italy., Sai-Kachout S; Laboratoire des Productions Animales et Fourragères, Institut National de la Recherche Agronomique de Tunisie, Université de Carthage, Rue Hédi Karray El Menzah, 1004, Tunis, Tunisia., Chtourou-Ghorbel N; Faculté des Sciences de Tunis, Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie (LR99ES12), Université de Tunis El Manar, Campus Universitaire Farhat Hached, 2092, El Manar, Tunis, Tunisia. nidhal.ghorbel@issbat.utm.tn.
Πηγή: Biochemical genetics [Biochem Genet] 2026 Aug; Vol. 64 (4), pp. 5843-5867. Date of Electronic Publication: 2026 Jan 20.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Kluwer Academic/Plenum Publishers Country of Publication: United States NLM ID: 0126611 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-4927 (Electronic) Linking ISSN: 00062928 NLM ISO Abbreviation: Biochem Genet Subsets: MEDLINE
Imprint Name(s): Publication: 1999- : New York : Kluwer Academic/Plenum Publishers
Original Publication: New York, Plenum Press.
Ιατρικοί όροι (MeSH): Festuca*/genetics , Genetic Variation* , Polymorphism, Genetic*, Tunisia ; Genetic Markers ; Genetics, Population ; Phylogeny
Περίληψη: Tall fescue (Festuca arundinacea Schreb.), taxonomically synonymous with Lolium arundinaceum (Schreb.) Darbysh., is a cool-season perennial grass valued for adaptability, forage quality, and stress tolerance. This study evaluated the genetic diversity and population structure of 32 Tunisian spontaneous populations and 3 local varieties using CAAT-box Derived Polymorphism (CBDP) and Conserved DNA-Derived Polymorphism (CDDP) markers. Out of 16 tested primers (8 CBDP and 8 CDDP), 12 (7 CBDP and 5 CDDP) generated reproducible and polymorphic banding patterns, producing a total of 124 bands for CBDP and 121 bands for CDDP. High polymorphism was observed (86.29% for CBDP; 74.38% for CDDP), with a mean polymorphism information content (PIC) of 0.378, confirming the informativeness of both marker systems. Clustering analyses based on CBDP, CDDP, and combined data revealed some grouping tendencies among populations, but these groupings were not strictly associated with geographic origin, bioclimatic stage, or whether populations were spontaneous or cultivated. STRUCTURE analysis suggested two clusters for each marker system, but accessions showed overlapping membership, indicating weak population structure. The combined dataset detected four admixed groups, yet all populations displayed high admixture, reflecting high genetic diversity and extensive gene flow in this outcrossing species. Analysis of molecular variance (AMOVA) indicated that over 97% of the total genetic variation occurred within regions, while PhiPT values were low but significant (P = 0.004). No isolation by distance was detected with the Mantel test (CBDP: r = 0.011, p = 0.419; CDDP: r = 0.087, p = 0.078). This study is the first to apply CBDP and CDDP markers in Tunisian tall fescue populations, demonstrating their efficiency in revealing genetic diversity. The findings highlight substantial genetic diversity among populations within regions and underscore the value of this germplasm for breeding and conservation efforts.
(© 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Competing Interests: Declarations. Conflict of interest: The authors declare no competing interests.
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Contributed Indexing: Keywords: CBDP; CDDP; Gene flow; Genetic variation; Population structure; Tall fescue
Substance Nomenclature: 0 (Genetic Markers)
Entry Date(s): Date Created: 20260120 Date Completed: 20260721 Latest Revision: 20260721
Update Code: 20260721
DOI: 10.1007/s10528-026-11318-0
PMID: 41557072
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1573-4927
DOI:10.1007/s10528-026-11318-0