Academic Journal
Development of an efficient in vitro micropropagation and biochemical profiling of Salvia halophila, an endemic Turkish sage.
| Τίτλος: | Development of an efficient in vitro micropropagation and biochemical profiling of Salvia halophila, an endemic Turkish sage. |
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| Συγγραφείς: | Tanur Erkoyuncu M; Department of Field Crops, Faculty of Agriculture, Selçuk University, Konya, 42130, Türkiye. mtanur@selcuk.edu.tr. |
| Πηγή: | BMC plant biology [BMC Plant Biol] 2026 Jan 19; Vol. 26 (1), pp. 289. Date of Electronic Publication: 2026 Jan 19. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: BioMed Central Country of Publication: England NLM ID: 100967807 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2229 (Electronic) Linking ISSN: 14712229 NLM ISO Abbreviation: BMC Plant Biol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: London : BioMed Central, [2001- |
| Ιατρικοί όροι (MeSH): | Salvia*/growth & development , Salvia*/metabolism , Salvia*/drug effects, Gibberellins/pharmacology ; Germination/drug effects ; Plant Shoots/growth & development ; Plant Shoots/drug effects ; Plant Shoots/metabolism ; Plant Growth Regulators/pharmacology ; Seeds/growth & development ; Turkey ; Culture Media |
| Περίληψη: | BACKGROUND: Salvia halophila Hedge is an endemic sage species confined to the salt steppe ecosystems of Central Anatolia (Türkiye), particularly around the Lake Tuz Basin, and is classified as Endangered (EN) by the IUCN. Its narrow distribution, low seed germination rates, and habitat pressures threaten its survival and restrict the sustainable exploitation of its valuable phytochemicals, such as rosmarinic acid and other phenolics. In vitro micropropagation represents a strategic approach for producing genetically uniform and phytochemically rich plant material, thereby supporting both ex situ conservation and biotechnological applications. RESULTS: Three germination strategies were compared. Chemical priming and direct gibberellic acid (GA₃) applications were largely ineffective, whereas in vitro germination on GA₃-enriched media produced viable seedlings. WPM medium supplemented with 0.5–1.0 mg L⁻¹ GA₃ achieved the highest germination rate (20%). The optimized sterilization treatment minimized fungal contamination (4.4%) while sustaining a high proportion of healthy shoots (73%). For shoot induction, MS medium consistently outperformed WPM, achieving 78% induction and 1.51 shoots per explant versus 50% and 0.86 on WPM. Among cytokinins, meta-topolin (mT) provided the most reliable results, ensuring 90–100% shoot induction with high shoot quality at 0.2–1.0 mg L⁻¹. Rooting experiments demonstrated the superiority of IBA over NAA, with 0.5 mg L⁻¹ IBA yielding 66% rooting and 77% acclimatization. Biochemical profiling showed that micropropagated plantlets accumulated higher phenolic contents (TPC = 15.9 mg GAE g⁻¹ DW) and stronger antioxidant capacity (IC₅₀=24.6 µg mL⁻¹) compared with seed-derived plants. CONCLUSION: The study successfully established a stage-specific and efficient micropropagation protocol for S. halophila. While WPM medium was more favorable for seed germination, MS medium supplemented with mT and IBA was optimal for shoot regeneration and rooting. The protocol not only enhances propagation efficiency but also increases secondary metabolite accumulation, providing a reliable platform for conservation, sustainable utilization, and potential commercial production of this endangered halophyte. |
| Competing Interests: | Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. |
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| Contributed Indexing: | Keywords: Acclimatization; Antioxidant activity; Basal medium; Cytokinins; Micropropagation; Phenolic compounds; Rooting; Salvia halophila |
| Substance Nomenclature: | 0 (Gibberellins) 0 (Plant Growth Regulators) BU0A7MWB6L (gibberellic acid) 0 (Culture Media) |
| Entry Date(s): | Date Created: 20260119 Date Completed: 20260627 Latest Revision: 20260627 |
| Update Code: | 20260627 |
| PubMed Central ID: | PMC12896189 |
| DOI: | 10.1186/s12870-026-08152-2 |
| PMID: | 41555254 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1471-2229 |
|---|---|
| DOI: | 10.1186/s12870-026-08152-2 |