Academic Journal
An inertial-acoustofluidic platform for bioanalysis: concurrent, high-precision, high-throughput separation of cells and bacteria.
| Τίτλος: | An inertial-acoustofluidic platform for bioanalysis: concurrent, high-precision, high-throughput separation of cells and bacteria. |
|---|---|
| Συγγραφείς: | Wang Z; State Key Laboratory of Extreme Environment Optoelectronic Dynamic Measurement Technology and Instrument, North University of China, Taiyuan, Shanxi, China., Liu X; State Key Laboratory of Extreme Environment Optoelectronic Dynamic Measurement Technology and Instrument, North University of China, Taiyuan, Shanxi, China., Duan J; State Key Laboratory of Extreme Environment Optoelectronic Dynamic Measurement Technology and Instrument, North University of China, Taiyuan, Shanxi, China., Zhang B; State Key Laboratory of Extreme Environment Optoelectronic Dynamic Measurement Technology and Instrument, North University of China, Taiyuan, Shanxi, China. zhangbinzhen@nuc.edu.cn., Li Y; Department of Nephrology, Shanxi Provincial People's Hospital, Taiyuan, Shanxi, China.; The Fifth Clinical Medical College of Shanxi Medical University, Taiyuan, Shanxi, China.; Postdoctoral Workstation, Shanxi Provincial People's Hospital, Taiyuan, Shanxi, China., Yang L; State Key Laboratory of Extreme Environment Optoelectronic Dynamic Measurement Technology and Instrument, North University of China, Taiyuan, Shanxi, China., Wang H; State Key Laboratory of Extreme Environment Optoelectronic Dynamic Measurement Technology and Instrument, North University of China, Taiyuan, Shanxi, China. |
| Πηγή: | Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2026 Aug; Vol. 418 (15), pp. 4709-4724. Date of Electronic Publication: 2026 Jan 07. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101134327 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-2650 (Electronic) Linking ISSN: 16182642 NLM ISO Abbreviation: Anal Bioanal Chem Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Heidelberg : Springer-Verlag, 2002- |
| Ιατρικοί όροι (MeSH): | Escherichia coli*/isolation & purification , Microfluidic Analytical Techniques*/instrumentation , Microfluidic Analytical Techniques*/methods , Cell Separation*/instrumentation , Cell Separation*/methods , Acoustics*/instrumentation, Humans ; Equipment Design ; Hep G2 Cells |
| Περίληψη: | Efficient separation of cancer cells from whole blood is vital for early cancer diagnosis. However, disease progression often causes complications that reduce survival, such as cholangitis in liver cancer due to bile-duct compression, with Escherichia coli (E. coli) and Klebsiella as common pathogens. To address this, we developed a sheathless microfluidic sorter that integrates inertial focusing with surface acoustic wave (SAW) manipulation to simultaneously isolate cancer cells and bacteria. Specifically, we introduce a label-free, SAW-driven microfluidic platform that couples passive inertial focusing with active SAW manipulation to enable concurrent separation of cancer cells and bacteria; Cr/Au interdigital transducers (IDTs) are patterned on a 128° Y-cut LiNbO₃ substrate. The system first employs a contraction-expansion microchannel to achieve preliminary separation. Larger particles are then deflected by a slanted interdigital transducer (S-IDT), improving bacterial sorting purity. Small particles are enriched at the first outlet (purity > 96%), and the remaining targets are further refined by a focusing interdigital transducer (F-IDT). Tests with polystyrene (PS) particles achieved >96% purity, and in clinical samples, sorting efficiency and purity exceeded 93% for HepG2 cells and E. coli, with HepG2 viability above 94%. This compact platform unifies active and passive mechanisms, offering promise for early diagnosis and real-time monitoring of complications. (© 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.) |
| Competing Interests: | Declarations. Conflict of interest: The authors declare no competing interests. |
| References: | Bray F, Laversanne M, Sung HYA, Ferlay J, Siegel RL, Soerjomataram I, et al. Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2024;74(3):229–63. (PMID: 38572751) European Assoc Study L. EASL clinical practice guidelines: management of hepatocellular carcinoma. J Hepatol. 2018;69(1):182–236. An Z, Braseth AL, Sahar N. Acute cholangitis: causes, diagnosis, and management. Gastroenterol Clin North Am. 2021;50(2):403–14. (PMID: 10.1016/j.gtc.2021.02.00534024448) Sulzer JK, Ocuin LM. Cholangitis causes, diagnosis, and management. Surg Clin North Am. 2019;99(2):175. (PMID: 10.1016/j.suc.2018.11.00230846028) Viehman JA, Clancy CJ, Clarke L, Shields RK, Silveira FP, Kwak EJ, et al. Surgical site infections after liver transplantation: emergence of multidrug-resistant bacteria and implications for prophylaxis and treatment strategies. Transplantation. 2016;100(10):2107–14. (PMID: 10.1097/TP.000000000000135627479167) Ding W, Ye W, Liu HY, Yang JB, Chu CX, Zhu HC, et al. High-gradient microstructured hybrid microfluidic chip for rare tumor cell capture. Anal Bioanal Chem. 2025;417(11):2361–74. (PMID: 10.1007/s00216-025-05825-z40085212) Wongkaew N. Nanofiber-integrated miniaturized systems: an intelligent platform for cancer diagnosis. Anal Bioanal Chem. 2019;411(19):4251–64. (PMID: 10.1007/s00216-019-01589-530706075) Mishra A, Dubash TD, Edd JF, Jewett MK, Garre SG, Karabacak NM, et al. Ultrahigh-throughput magnetic sorting of large blood volumes for epitope-agnostic isolation of circulating tumor cells. Proc Natl Acad Sci USA. 2020;117(29):16839–47. (PMID: 10.1073/pnas.2006388117326415157382214) Ma YN, Wang Q, Sun SH, Li YX, Wang R, Yang HH, et al. Selection and characterization of novel DNA aptamers targeting colorectal cancer cells with malignant potential. Anal Bioanal Chem. 2025;417(26):5941–55. (PMID: 10.1007/s00216-025-06091-940890503) Fina E, Cleris L, Dugo M, Lecchi M, Ciniselli CM, Lecis D, et al. Gene signatures of circulating breast cancer cell models are a source of novel molecular determinants of metastasis and improve circulating tumor cell detection in patients. J Exp Clin Cancer Res. 2022;41(1):78. (PMID: 10.1186/s13046-022-02259-8352166158876758) Li Y, Li Y, Wang X, Wang K, Li HL, Wang PF, et al. Label-free detection and simultaneous viability determination of CTCs by lens-free imaging cytometry. Anal Bioanal Chem. 2025;417(1):95–107. (PMID: 10.1007/s00216-024-05624-y39477899) Li WM, Ren XD, Jiang YZ, Su N, Li BW, Sun XG, et al. Rapid detection of EGFR mutation in CTCs based on a double spiral microfluidic chip and the real-time RPA method. Anal Bioanal Chem. 2023;415(17):3535–47. (PMID: 10.1007/s00216-023-04743-237254002) Dadgar N, Anees M, Sherry C, Park HY, Grayhack EE, Goel A, et al. Blood-based surveillance biomarkers for gastroesophageal cancers. Cancers (Basel). 2025. https://doi.org/10.3390/cancers17213552 . (PMID: 10.3390/cancers172135524122834612607582) Chen ZZ, Shen L, Zhao X, Chen HQ, Xiao YX, Zhang YH, et al. Acoustofluidic micromixers: from rational design to lab-on-a-chip applications. Appl Mater Today. 2022. https://doi.org/10.1016/j.apmt.2021.101356 . (PMID: 10.1016/j.apmt.2021.101356354342638994858) Abdulla A, Maboyi N, Ding X. Application of microfluidics in single-cell manipulation, omics and drug development. Curr Med Chem. 2021;28(40):8433–50. (PMID: 10.2174/092986732866621020320564133538663) Dong Z, Wang HF, Chen JB, Fang Q. A micropore array-based single-cell operating system. Sens Actuators B Chem. 2025;426:136993. (PMID: 10.1016/j.snb.2024.136993) Joensson HN, Svahn HA. Droplet microfluidics-a tool for single-cell analysis. Angew Chem Int Ed. 2012;51(49):12176–92. (PMID: 10.1002/anie.201200460) Kim SC, Haliburton JR, Gartner ZJ, Abate AR. Single-cell protein profiling by microdroplet barcoding and next-generation sequencing. Methods Mol Biol. 2022;2386:101–11. (PMID: 10.1007/978-1-0716-1771-7_7347662679122841) Yun JL, Zheng XW, Xu P, Zheng X, Xu JY, Cao C, et al. Interfacial nanoinjection-based nanoliter single-cell analysis. Small. 2020. https://doi.org/10.1002/smll.201903739 . (PMID: 10.1002/smll.20190373933373107) Zhang DX, Qiao L. Microfluidics coupled mass spectrometry for single cell multi-omics. Small Methods. 2024;8(1):2301179. (PMID: 10.1002/smtd.202301179) Zambrzycki SC, Kertesz V, Cahill JF. Evaluating inkjet dispenser/liquid vortex capture-mass spectrometry for single-cell metabolomics in Hep G2 steatosis caused by tamoxifen. Anal Bioanal Chem. 2025;417(16):3597–609. (PMID: 10.1007/s00216-025-05885-140323376) Lim SB, Menon NV, Lim CT. Microfluidic tools for probing micro-culprits opportunities and challenges in microfluidic diagnostics. EMBO Rep. 2020. https://doi.org/10.15252/embr.201949749 . (PMID: 10.15252/embr.201949749320433007132214) Gong YXY, Huang XF, Liang J, Xie J, Qing LS. Aptamer-based microfluidics for the detection of cancer biomarkers. Anal Bioanal Chem. 2025;417(16):3529–50. (PMID: 10.1007/s00216-025-05863-740227354) Mou L, Jiang XY. Materials for microfluidic immunoassays: a review. Adv Healthc Mater. 2017;6(15):1601403. (PMID: 10.1002/adhm.201601403) Dou MW, Sanjay ST, Benhabib M, Xu F, Li XJ. Low-cost bioanalysis on paper-based and its hybrid microfluidic platforms. Talanta. 2015;145:43–54. (PMID: 10.1016/j.talanta.2015.04.068264594424607929) Rondot N, Yan S, Mager D, Kulinsky L. A low-cost printed circuit board-based centrifugal microfluidic platform for dielectrophoresis. Microsyst Nanoeng. 2025. https://doi.org/10.1038/s41378-024-00856-5 . (PMID: 10.1038/s41378-024-00856-53986506911770146) Zhang YY, Zheng TT, Wang L, Feng L, Wang M, Zhang ZC, et al. From passive to active sorting in microfluidics: a review. Rev Adv Mater Sci. 2021;60(1):313–24. (PMID: 10.1515/rams-2020-0044) Lu Q, Zhang ZN, Ding XT. Isolation techniques of micro/nano-scaled species for biomedical applications. Adv Sci. 2025. https://doi.org/10.1002/advs.202414109 . (PMID: 10.1002/advs.202414109) Di Carlo D, Irimia D, Tompkins RG, Toner M. Continuous inertial focusing, ordering, and separation of particles in microchannels. Proc Natl Acad Sci USA. 2007;104(48):18892–7. (PMID: 10.1073/pnas.0704958104180254772141878) Richard C, Fakhfouri A, Colditz M, Striggow F, Kronstein-Wiedemann R, Tonn T, et al. Blood platelet enrichment in mass-producible surface acoustic wave (SAW) driven microfluidic chips. Lab Chip. 2019;19(24):4043–51. (PMID: 10.1039/C9LC00804G31723953) Al-Ali A, Waheed W, Abu-Nada E, Alazzam A. A review of active and passive hybrid systems based on dielectrophoresis for the manipulation of microparticles. J Chromatogr A. 2022;1676:463268. (PMID: 10.1016/j.chroma.2022.46326835779391) Li XK, Wang M, Davis TP, Zhang LW, Qiao RR. Advancing tissue culture with light-driven 3D-printed microfluidic devices. Biosensors. 2024;14(6):301. (PMID: 10.3390/bios140603013892060511201418) Ye Z, Sun B, Xiao Z. Artificial antigen-presenting immunomagnetic beads for better enrichment and expansion of T lymphocytes from peripheral blood mononuclear cells. J Chem Technol Biotechnol. 2020;95(6):1649–56. (PMID: 10.1002/jctb.6356) Sivaramakrishnan M, Kothandan R, Govindarajan DK, Meganathan Y, Kandaswamy K. Active microfluidic systems for cell sorting and separation. Curr Opin Biomed Eng. 2020;13:60–8. (PMID: 10.1016/j.cobme.2019.09.014) Li Y, Cai S, Shen H, Chen Y, Ge Z, Yang W. Recent advances in acoustic microfluidics and its exemplary applications. Biomicrofluidics. 2022. https://doi.org/10.1063/5.0089051 . (PMID: 10.1063/5.0089051363892739646254) Soller R, Jakobsson O, Augustsson P. In-line enrichment of cancer cells from whole blood by cell self-organization in acoustic fields. Anal Chem. 2025;97(25):13310–7. (PMID: 10.1021/acs.analchem.5c014594051477412224171) Wu M, Ozcelik A, Rufo J, Wang Z, Fang R, Jun Huang T. Acoustofluidic separation of cells and particles. Microsyst Nanoeng. 2019;5(1):32. https://doi.org/10.1038/s41378-019-0064-3 . Liang F, Zhu JW, Chai HC, Feng YX, Zhao P, Liu SF, et al. Non-invasive and minute-frequency 3D tomographic imaging enabling long-term spatiotemporal observation of single cell fate. Small Methods. 2023. https://doi.org/10.1002/smtd.202201492 . (PMID: 10.1002/smtd.20220149238037763) Ning S, Liu S, Xiao Y, Zhang G, Cui W, Reed M. A microfluidic chip with a serpentine channel enabling high-throughput cell separation using surface acoustic waves. Lab Chip. 2021;21(23):4608–17. (PMID: 10.1039/D1LC00840D34763349) Li P, Zhong J, Liu N, Lu X, Liang M, Ai Y. Physical properties-based microparticle sorting at submicron resolution using a tunable acoustofluidic device. Sens Actuators B Chem. 2021;344:130203. (PMID: 10.1016/j.snb.2021.130203) Gou Y, Jia Y, Wang P, Sun C. Progress of inertial microfluidics in principle and application. Sensors. 2018;18(6):1762. (PMID: 10.3390/s18061762298575636021949) Zhou T, He J, Wu Z, Bian Q, He X, Zhou S, et al. Dielectrophoretic–inertial microfluidics for Symbiodinium separation and enrichment. Phys Fluids. 2024. https://doi.org/10.1063/5.0193920 . (PMID: 10.1063/5.0193920) Zhou J, Papautsky I. Fundamentals of inertial focusing in microchannels. Lab Chip. 2013;13(6):1121–32. (PMID: 10.1039/c2lc41248a23353899) Zhou J, Giridhar PV, Kasper S, Papautsky I. Modulation of aspect ratio for complete separation in an inertial microfluidic channel. Lab Chip. 2013;13(10):1919–29. (PMID: 10.1039/c3lc50101a23529341) Wu Z, Chen Y, Wang M, Chung AJ. Continuous inertial microparticle and blood cell separation in straight channels with local microstructures. Lab Chip. 2016;16(3):532–42. (PMID: 10.1039/C5LC01435B26725506) Law P, Dooley DC, Alsop P, Smith DM, Landmark JD, Meryman HT. Density gradient isolation of peripheral blood mononuclear cells using a blood cell processor. Transfusion. 1988;28(2):145–50. (PMID: 10.1046/j.1537-2995.1988.28288179019.x3354042) Weiss R, Gerdes W, Berthold R, Sack U, Koehl U, Hauschildt S, et al. Comparison of three CD3-specific separation methods leading to labeled and label-free T cells. Cells. 2021. https://doi.org/10.3390/cells10112824 . (PMID: 10.3390/cells10112824349438268699345) Matsumoto M, Tashiro S, Ito T, Takahashi K, Hashimoto G, Kajihara J, et al. Fully closed cell sorter for immune cell therapy manufacturing. Mol Ther. 2023;30:367–76. Cha H, Kang X, Yuan D, de Villiers B, Mak J, Nguyen N-T, et al. High-efficient white blood cell separation from whole blood using cascaded inertial microfluidics. Talanta. 2025;284:127200. (PMID: 10.1016/j.talanta.2024.12720039581104) Wang M-H, Lee Y-C, Tu T-Y, Huang P-c, Jang L-S, Chen C-H. Optimization analysis of the particle focusing and separation parameters of a sheathless microfluidic device using standing surface acoustic waves. Phys Fluids. 2024. https://doi.org/10.1063/5.0238560 . (PMID: 10.1063/5.0238560) |
| Grant Information: | No. 2023-SYSJJ-03 Research Fund of the Key Laboratory of North University of China; No. 52175555 National Natural Science Foundation of China; Grant No. 2022-143 China Scholarship Council of Shanxi Province |
| Contributed Indexing: | Keywords: Acoustofluidic; Cell and bacteria separation; Inertial focusing; Liver cancer liquid biopsy; Microfluidic sorting; Surface acoustic waves |
| Entry Date(s): | Date Created: 20260107 Date Completed: 20260721 Latest Revision: 20260721 |
| Update Code: | 20260721 |
| DOI: | 10.1007/s00216-025-06286-0 |
| PMID: | 41498815 |
| Βάση Δεδομένων: | MEDLINE |
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