Academic Journal
Impact of pH on Intratympanic Sodium Thiosulfate-hyaluronan Gel in Preventing Cisplatin-induced Ototoxicity.
| Τίτλος: | Impact of pH on Intratympanic Sodium Thiosulfate-hyaluronan Gel in Preventing Cisplatin-induced Ototoxicity. |
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| Συγγραφείς: | Videhult Pierre P; Department of Clinical Science, Intervention and Technology, Division of Audiology, Karolinska Institutet, Huddinge, Sweden., Fransson A; Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden., Laurell G; Department of Surgical Sciences, Uppsala University, Uppsala, Sweden. |
| Πηγή: | Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology [Otol Neurotol] 2026 Mar 01; Vol. 47 (3), pp. e515-e521. Date of Electronic Publication: 2026 Jan 07. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 100961504 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1537-4505 (Electronic) Linking ISSN: 15317129 NLM ISO Abbreviation: Otol Neurotol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Hagerstown, MD : Lippincott Williams & Wilkins, c2001- |
| Ιατρικοί όροι (MeSH): | Cisplatin*/toxicity , Cisplatin*/adverse effects , Thiosulfates*/administration & dosage , Thiosulfates*/pharmacology , Hyaluronic Acid*/administration & dosage , Hyaluronic Acid*/pharmacology , Ototoxicity*/prevention & control , Antineoplastic Agents*/toxicity, Cochlea/drug effects ; Cochlea/pathology ; Hair Cells, Auditory, Outer/drug effects ; Hair Cells, Auditory, Outer/pathology ; Animals ; Guinea Pigs ; Hydrogen-Ion Concentration ; Gels ; Injection, Intratympanic ; Male |
| Περίληψη: | Hypothesis: The pH of an intratympanic gel influences the level of protection against the ototoxic effects of systemic cisplatin. Background: Nucleophiles reduce cisplatin-induced ototoxicity by chemically inactivating cisplatin and counteracting oxidative stress. pH is a factor influencing cisplatin's reactivity. Here, intratympanic administration of a hyaluronan-based gel containing the nucleophile sodium thiosulfate (STS) was used to investigate the role of pH in otoprotection. Methods: Guinea pigs (n=32) received a unilateral transtympanic injection of a hyaluronan-based gel (0.10 to 0.15 mL) with either STS at pH 6.5 or pH 8.0, or NaCl at pH 6.5 or pH 8.0. The contralateral ear served as control. After 30 minutes, cisplatin (8 mg/kg b.w., i.v.) was administered. Cochleae were harvested 4 days later for hair cell quantification. Results: Mean percentage of outer hair cell (OHC) loss per mm in the region ≤10 mm from the round window had a median absolute intraindividual difference of 6.0% (quartiles 1 and 2: 3.8% to 11%; P =0.012; n=8) in group STS pH 6.5 and 5.8% (1.8% to 18%; P =0.046; n=6) in group STS pH 8.0, favoring the gel-treated ear. The corresponding numbers in the NaCl groups were -0.2% (-2.2 to 8.7%; P =1.000; n=8) in group NaCl pH 6.5 and 3.3% (-8.1% to 22%; P =0.237; n=7) in group NaCl pH 8.0. The difference between nontreated and gel-treated ears did not differ significantly between the 2 STS groups. Conclusion: STS in a hyaluronan-based gel reduced OHC loss equally well at both pH levels, while NaCl was ineffective. (Copyright © 2026 The Author(s). Published by Wolters Kluwer Health, Inc on behalf of Otology & Neurotology, Inc.) |
| Competing Interests: | The authors disclose no conflicts of interest. |
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| Grant Information: | 2016-532 Hörselforskningsfonden; FB16-0020 Stiftelsen Tysta Skolan |
| Contributed Indexing: | Keywords: Cisplatin; Guinea pig; Hair cell loss; Hearing damage; Hearing protection; MeSH; Sodium thiosulfate; Transtympanic |
| Substance Nomenclature: | Q20Q21Q62J (Cisplatin) 0 (Thiosulfates) 9004-61-9 (Hyaluronic Acid) HX1032V43M (sodium thiosulfate) 0 (Gels) 0 (Antineoplastic Agents) |
| Entry Date(s): | Date Created: 20260106 Date Completed: 20260211 Latest Revision: 20260211 |
| Update Code: | 20260211 |
| PubMed Central ID: | PMC12879179 |
| DOI: | 10.1097/MAO.0000000000004800 |
| PMID: | 41493913 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1537-4505 |
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| DOI: | 10.1097/MAO.0000000000004800 |