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Molecular identification of Aelurostrongylus abstrusus, Troglostrongylus brevior and Angiostrongylus chabaudi in domestic cats from the Azores Islands (Portugal).

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Title: Molecular identification of Aelurostrongylus abstrusus, Troglostrongylus brevior and Angiostrongylus chabaudi in domestic cats from the Azores Islands (Portugal).
Authors: Teixeira R; Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, Lisbon University, (CIISA-FMV-ULisboa), Avenida da Universidade Técnica, Lisboa 1300-477, Portugal; Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Lisbon 1300-477, Portugal. Electronic address: romanateixeira@edu.ulisboa.pt., Valério-Bolas A; Genevet - Veterinary Molecular Diagnostic Laboratory (LDMV Genevet), Carnaxide 2790-140, Portugal., Flor I; Laboratory of Parasitology, Regional Veterinary Laboratory, Vinha Brava, Angra do Heroísmo 9700-236, Portugal., Pinto C; Faculty of Agricultural and Environmental Sciences, University of the Azores, Angra do Heroísmo 9700-042, Portugal., de Carvalho LM; Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, Lisbon University, (CIISA-FMV-ULisboa), Avenida da Universidade Técnica, Lisboa 1300-477, Portugal; Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Lisbon 1300-477, Portugal., Pomba MC; Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, Lisbon University, (CIISA-FMV-ULisboa), Avenida da Universidade Técnica, Lisboa 1300-477, Portugal; Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Lisbon 1300-477, Portugal; Genevet - Veterinary Molecular Diagnostic Laboratory (LDMV Genevet), Carnaxide 2790-140, Portugal.
Source: Molecular and biochemical parasitology [Mol Biochem Parasitol] 2026 Feb; Vol. 265, pp. 111721. Date of Electronic Publication: 2025 Dec 20.
Publication Type: Journal Article
Language: English
Journal Info: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8006324 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-9428 (Electronic) Linking ISSN: 01666851 NLM ISO Abbreviation: Mol Biochem Parasitol Subsets: MEDLINE
Imprint Name(s): Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press.
MeSH Terms: Cat Diseases*/parasitology , Cat Diseases*/epidemiology , Strongylida Infections*/veterinary , Strongylida Infections*/parasitology , Strongylida Infections*/epidemiology , Angiostrongylus*/genetics , Angiostrongylus*/isolation & purification , Angiostrongylus*/classification , Metastrongyloidea*/genetics , Metastrongyloidea*/classification , Metastrongyloidea*/isolation & purification, Azores/epidemiology ; Feces/parasitology ; Portugal/epidemiology ; Animals ; Cats ; Polymerase Chain Reaction ; Phylogeny
Abstract: Domestic cats can be infected with various cardiopulmonary metastrongylids. Although A. abstrusus is widely distributed globally, data regarding the occurrence of T. brevior and A. chaubaudi in Portugal are currently nonexistent. This study aimed to evaluate the presence of cardiopulmonary parasite species in domestic cats from the Azores, Portugal, using copromicroscopy and molecular methods. A total of 57 domestic cats were included in this study, and fecal samples were previously analyzed using the Baermann technique. The detected larvae were collected, morphologically identified, and subsequently confirmed through molecular analysis using triplex semi-nested PCR. 57 domestic cats tested positive for cardiopulmonary parasites by copromicroscopy. Triplex semi-nested PCR analysis confirmed the presence of A. abstrusus (326 bp), T. brevior (520 bp) and A. chabaudi (200 bp) in the Azores archipelago. The present study is the first to molecularly confirm A. abstrusus, T. brevior, and A. chabaudi in domestic cats from Portugal and the first molecular report of domestic cats from the Azores Islands. Future studies are recommended to further investigate the distribution and epidemiology of these parasites in felines.
(Copyright © 2025 The Authors. Published by Elsevier B.V. All rights reserved.)
Competing Interests: Declaration of Competing Interest The authors declare no conflicts of interest related to this study.
Contributed Indexing: Keywords: A. abstrusus; A. chaubaudi; Azores; Lungworms; T. brevior
Entry Date(s): Date Created: 20251222 Date Completed: 20260124 Latest Revision: 20260701
Update Code: 20260701
DOI: 10.1016/j.molbiopara.2025.111721
PMID: 41429192
Database: MEDLINE
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  Data: Molecular identification of Aelurostrongylus abstrusus, Troglostrongylus brevior and Angiostrongylus chabaudi in domestic cats from the Azores Islands (Portugal).
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  Data: <searchLink fieldCode="AU" term="%22Teixeira+R%22">Teixeira R</searchLink>; Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, Lisbon University, (CIISA-FMV-ULisboa), Avenida da Universidade Técnica, Lisboa 1300-477, Portugal; Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Lisbon 1300-477, Portugal. Electronic address: romanateixeira@edu.ulisboa.pt.<br /><searchLink fieldCode="AU" term="%22Valério-Bolas+A%22">Valério-Bolas A</searchLink>; Genevet - Veterinary Molecular Diagnostic Laboratory (LDMV Genevet), Carnaxide 2790-140, Portugal.<br /><searchLink fieldCode="AU" term="%22Flor+I%22">Flor I</searchLink>; Laboratory of Parasitology, Regional Veterinary Laboratory, Vinha Brava, Angra do Heroísmo 9700-236, Portugal.<br /><searchLink fieldCode="AU" term="%22Pinto+C%22">Pinto C</searchLink>; Faculty of Agricultural and Environmental Sciences, University of the Azores, Angra do Heroísmo 9700-042, Portugal.<br /><searchLink fieldCode="AU" term="%22de+Carvalho+LM%22">de Carvalho LM</searchLink>; Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, Lisbon University, (CIISA-FMV-ULisboa), Avenida da Universidade Técnica, Lisboa 1300-477, Portugal; Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Lisbon 1300-477, Portugal.<br /><searchLink fieldCode="AU" term="%22Pomba+MC%22">Pomba MC</searchLink>; Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, Lisbon University, (CIISA-FMV-ULisboa), Avenida da Universidade Técnica, Lisboa 1300-477, Portugal; Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Lisbon 1300-477, Portugal; Genevet - Veterinary Molecular Diagnostic Laboratory (LDMV Genevet), Carnaxide 2790-140, Portugal.
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  Data: <searchLink fieldCode="MM" term="%22Cat+Diseases%22">Cat Diseases*</searchLink>/<searchLink fieldCode="MM" term="%22Cat+Diseases+parasitology%22">parasitology</searchLink> <br /><searchLink fieldCode="MM" term="%22Cat+Diseases%22">Cat Diseases*</searchLink>/<searchLink fieldCode="MM" term="%22Cat+Diseases+epidemiology%22">epidemiology</searchLink> <br /><searchLink fieldCode="MM" term="%22Strongylida+Infections%22">Strongylida Infections*</searchLink>/<searchLink fieldCode="MM" term="%22Strongylida+Infections+veterinary%22">veterinary</searchLink> <br /><searchLink fieldCode="MM" term="%22Strongylida+Infections%22">Strongylida Infections*</searchLink>/<searchLink fieldCode="MM" term="%22Strongylida+Infections+parasitology%22">parasitology</searchLink> <br /><searchLink fieldCode="MM" term="%22Strongylida+Infections%22">Strongylida Infections*</searchLink>/<searchLink fieldCode="MM" term="%22Strongylida+Infections+epidemiology%22">epidemiology</searchLink> <br /><searchLink fieldCode="MM" term="%22Angiostrongylus%22">Angiostrongylus*</searchLink>/<searchLink fieldCode="MM" term="%22Angiostrongylus+genetics%22">genetics</searchLink> <br /><searchLink fieldCode="MM" term="%22Angiostrongylus%22">Angiostrongylus*</searchLink>/<searchLink fieldCode="MM" term="%22Angiostrongylus+isolation+%26+purification%22">isolation & purification</searchLink> <br /><searchLink fieldCode="MM" term="%22Angiostrongylus%22">Angiostrongylus*</searchLink>/<searchLink fieldCode="MM" term="%22Angiostrongylus+classification%22">classification</searchLink> <br /><searchLink fieldCode="MM" term="%22Metastrongyloidea%22">Metastrongyloidea*</searchLink>/<searchLink fieldCode="MM" term="%22Metastrongyloidea+genetics%22">genetics</searchLink> <br /><searchLink fieldCode="MM" term="%22Metastrongyloidea%22">Metastrongyloidea*</searchLink>/<searchLink fieldCode="MM" term="%22Metastrongyloidea+classification%22">classification</searchLink> <br /><searchLink fieldCode="MM" term="%22Metastrongyloidea%22">Metastrongyloidea*</searchLink>/<searchLink fieldCode="MM" term="%22Metastrongyloidea+isolation+%26+purification%22">isolation & purification</searchLink><br /><searchLink fieldCode="MH" term="%22Azores%22">Azores</searchLink>/<searchLink fieldCode="MH" term="%22Azores+epidemiology%22">epidemiology</searchLink> ; <searchLink fieldCode="MH" term="%22Feces%22">Feces</searchLink>/<searchLink fieldCode="MH" term="%22Feces+parasitology%22">parasitology</searchLink> ; <searchLink fieldCode="MH" term="%22Portugal%22">Portugal</searchLink>/<searchLink fieldCode="MH" term="%22Portugal+epidemiology%22">epidemiology</searchLink> ; <searchLink fieldCode="MH" term="%22Animals%22">Animals</searchLink> ; <searchLink fieldCode="MH" term="%22Cats%22">Cats</searchLink> ; <searchLink fieldCode="MH" term="%22Polymerase+Chain+Reaction%22">Polymerase Chain Reaction</searchLink> ; <searchLink fieldCode="MH" term="%22Phylogeny%22">Phylogeny</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Domestic cats can be infected with various cardiopulmonary metastrongylids. Although A. abstrusus is widely distributed globally, data regarding the occurrence of T. brevior and A. chaubaudi in Portugal are currently nonexistent. This study aimed to evaluate the presence of cardiopulmonary parasite species in domestic cats from the Azores, Portugal, using copromicroscopy and molecular methods. A total of 57 domestic cats were included in this study, and fecal samples were previously analyzed using the Baermann technique. The detected larvae were collected, morphologically identified, and subsequently confirmed through molecular analysis using triplex semi-nested PCR. 57 domestic cats tested positive for cardiopulmonary parasites by copromicroscopy. Triplex semi-nested PCR analysis confirmed the presence of A. abstrusus (326 bp), T. brevior (520 bp) and A. chabaudi (200 bp) in the Azores archipelago. The present study is the first to molecularly confirm A. abstrusus, T. brevior, and A. chabaudi in domestic cats from Portugal and the first molecular report of domestic cats from the Azores Islands. Future studies are recommended to further investigate the distribution and epidemiology of these parasites in felines.<br /> (Copyright © 2025 The Authors. Published by Elsevier B.V. All rights reserved.)
– Name: Abstract
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  Data: Declaration of Competing Interest The authors declare no conflicts of interest related to this study.
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  Data: <i>Keywords: </i>A. abstrusus; A. chaubaudi; Azores; Lungworms; T. brevior
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        Text: English
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      – SubjectFull: Portugal epidemiology
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      – TitleFull: Molecular identification of Aelurostrongylus abstrusus, Troglostrongylus brevior and Angiostrongylus chabaudi in domestic cats from the Azores Islands (Portugal).
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