Academic Journal
Urease-Powered Nanomotors for Enhanced Mucosal and Tumor Penetration To Augment Sonodynamic Therapy in Bladder Cancer.
| Title: | Urease-Powered Nanomotors for Enhanced Mucosal and Tumor Penetration To Augment Sonodynamic Therapy in Bladder Cancer. |
|---|---|
| Authors: | Wu R; Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P. R. China.; Department of Urology, The Second Affiliated Hospital, Army Medical University, Chongqing 400038, P. R. China., Liao R; Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P. R. China.; Department of Urology, The Second Affiliated Hospital of Chengdu Medical College, China National Nuclear Corporation 416 Hospital, Chengdu 610051, P. R. China., Yin H; Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P. R. China., He W; Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P. R. China. |
| Source: | ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2025 Dec 24; Vol. 17 (51), pp. 69017-69031. Date of Electronic Publication: 2025 Dec 15. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101504991 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1944-8252 (Electronic) Linking ISSN: 19448244 NLM ISO Abbreviation: ACS Appl Mater Interfaces Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Washington, D.C. : American Chemical Society |
| MeSH Terms: | Urinary Bladder Neoplasms*/therapy , Urinary Bladder Neoplasms*/pathology , Urinary Bladder Neoplasms*/metabolism , Urinary Bladder Neoplasms*/drug therapy , Urease*/chemistry , Urease*/metabolism , Nanoparticles*/chemistry , Ultrasonic Therapy*, Mucous Membrane/metabolism ; Porphyrins/chemistry ; Animals ; Mice ; Humans ; Cell Line, Tumor ; Female |
| Abstract: | Sonodynamic therapy (SDT) represents a promising minimally invasive approach for treating bladder cancer, however, its efficacy following intravesical instillation is substantially compromised by the glycosaminoglycan-rich mucosal barrier and the cyclic voiding process of the bladder. To address these limitations, nanomotors coloaded with enzyme and sonosensitizer were engineered to enhance self-propelled tissue penetration and prolong retention of SDT for bladder cancer. Herein, urine-derived exosomes (EXO) were isolated using density gradient ultracentrifugation and subsequently engineered into EXO@UT nanovesicles (NVs) via electroporation-mediated coencapsulation of urease and tetrakis (4-carboxyphenyl) porphyrin. The NVs catalyze the decomposition of urea within the bladder to generate carbon dioxide and ammonia, thereby propelling their migration toward the bladder mucosa and circumventing washout during micturition as well as enhanced retention within tumor. Following intravesical administration in mice bearing bladder cancer, the NVs with self-propulsion demonstrated significantly enhanced infiltration into mucosal tissues and improved drug accumulation at the tumor site, compared to static counterpart which remained largely localized on the mucosal surface. The self-propelled NVs for SDT activated by low-intensity ultrasound irradiation demonstrated significantly stronger tumor growth suppression and extended animal survival compared to those exposed to external near-infrared illumination. Thus, this study presents a robust therapeutic platform based on self-propelled NVs that effectively overcome mucosal barriers and potentiate SDT, offering a promising strategy for enhanced treatment of bladder cancer. |
| Contributed Indexing: | Keywords: bladder cancer; mucosal infiltration; self-propulsion; sonodynamic therapy; tumor penetration |
| Substance Nomenclature: | EC 3.5.1.5 (Urease) 0 (Porphyrins) |
| Entry Date(s): | Date Created: 20251215 Date Completed: 20251230 Latest Revision: 20251230 |
| Update Code: | 20260130 |
| DOI: | 10.1021/acsami.5c17665 |
| PMID: | 41395784 |
| Database: | MEDLINE |
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