Academic Journal
Mucosal Brush Sampling Increases the Helicobacter pylori Detection Rate of the Rapid Urease Test: A Pilot Study.
| Title: | Mucosal Brush Sampling Increases the Helicobacter pylori Detection Rate of the Rapid Urease Test: A Pilot Study. |
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| Authors: | Bang CS; Institute of New Frontier Research, Hallym University College of Medicine, Chuncheon, Korea.; Department of Internal Medicine, Hallym University College of Medicine, Chuncheon, Korea., Choi AI; Institute of New Frontier Research, Hallym University College of Medicine, Chuncheon, Korea., Choi JG; Institute of New Frontier Research, Hallym University College of Medicine, Chuncheon, Korea., Lee JJ; Institute of New Frontier Research, Hallym University College of Medicine, Chuncheon, Korea.; Department of Anesthesiology and Pain Medicine, Hallym University College of Medicine, Chuncheon, Korea., Gong EJ; Institute of New Frontier Research, Hallym University College of Medicine, Chuncheon, Korea.; Department of Internal Medicine, Hallym University College of Medicine, Chuncheon, Korea. |
| Source: | Gut and liver [Gut Liver] 2026 Jan 15; Vol. 20 (1), pp. 77-85. Date of Electronic Publication: 2025 Nov 27. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Editorial Office of Gut and Liver Country of Publication: Korea (South) NLM ID: 101316452 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2005-1212 (Electronic) Linking ISSN: 19762283 NLM ISO Abbreviation: Gut Liver Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Seoul : Editorial Office of Gut and Liver |
| MeSH Terms: | Helicobacter pylori*/isolation & purification , Helicobacter pylori*/enzymology , Helicobacter Infections*/diagnosis , Helicobacter Infections*/microbiology , Urease*/analysis , Gastric Mucosa*/microbiology , Gastric Mucosa*/pathology, Biopsy/methods ; Humans ; Pilot Projects ; Male ; Female ; Middle Aged ; Adult ; Aged ; Breath Tests ; Sensitivity and Specificity |
| Abstract: | Background/aims: Accurate diagnosis of and timely eradication therapy for Helicobacter pylori are crucial for managing and preventing adverse clinical outcomes associated with H. pylori infection. H. pylori infection is typically diagnosed using endoscopic biopsy-based tests such as the rapid urease test (RUT). In this study, we investigated the usefulness of mucosal brush sampling for H. pylori detection using the RUT and culture. Methods: Twenty patients with H. pylori infection underwent endoscopy, and specimens were collected from the greater curvature of the gastric corpus via both mucosal brush and biopsy sampling methods. Brushing was performed using a disposable cytology brush, and the brush specimen was used for the RUT (brush-RUT) and then for culture. Two biopsies were obtained for the RUT (biopsy-RUT) and culture. H. pylori detection rates using RUT and culture yields from brush and biopsy samples were compared. Results: The H. pylori detection rate was 100% with the brush-RUT, whereas it was 75% with the biopsy-RUT. Notably, among patients taking acid-suppressive agents, the sensitivity of the biopsy-RUT decreased to 66.7%, whereas that of the brush-RUT remained at 100%. The biopsy-RUT yield was also associated with the delta over baseline value determined by the urea breath test. H. pylori was successfully isolated from all the biopsy specimens and 95% of the brush samples, and all H. pylori isolates were tested for antimicrobial susceptibility. No significant procedure-related adverse events occurred with either sampling method. Conclusions: Mucosal brushing is a simple, effective, and highly sensitive diagnostic method for H. pylori infection. The mucosal brush method is a practical alternative to biopsy, expanding the diagnostic capabilities while minimizing invasiveness. |
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| Contributed Indexing: | Keywords: Culture; Diagnosis; Helicobacter pylori; Proton pump inhibitors |
| Substance Nomenclature: | EC 3.5.1.5 (Urease) |
| Entry Date(s): | Date Created: 20251127 Date Completed: 20260113 Latest Revision: 20260420 |
| Update Code: | 20260421 |
| PubMed Central ID: | PMC12800670 |
| DOI: | 10.5009/gnl250332 |
| PMID: | 41306096 |
| Database: | MEDLINE |
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