Academic Journal
Mineral Oil Encapsulation Enhances dsRNA-Based Acaricidal Activity against Amphitetranychus viennensis.
| Τίτλος: | Mineral Oil Encapsulation Enhances dsRNA-Based Acaricidal Activity against Amphitetranychus viennensis. |
|---|---|
| Συγγραφείς: | Zhang P; Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China.; College of Plant Protection, Shanxi Agricultural University, Taiyuan, Shanxi 030031, China., Zhang G; College of Plant Protection, Shanxi Agricultural University, Taiyuan, Shanxi 030031, China., He L; College of Plant Protection, Shanxi Agricultural University, Taiyuan, Shanxi 030031, China., Liu Z; College of Plant Protection, Shanxi Agricultural University, Taiyuan, Shanxi 030031, China., Yang J; College of Plant Protection, Shanxi Agricultural University, Taiyuan, Shanxi 030031, China., Gao Y; College of Plant Protection, Shanxi Agricultural University, Taiyuan, Shanxi 030031, China., Fan R; College of Plant Protection, Shanxi Agricultural University, Taiyuan, Shanxi 030031, China., Du F; Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China. |
| Πηγή: | ACS applied bio materials [ACS Appl Bio Mater] 2025 Dec 15; Vol. 8 (12), pp. 11155-11166. Date of Electronic Publication: 2025 Nov 14. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: ACS Publications Country of Publication: United States NLM ID: 101729147 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2576-6422 (Electronic) Linking ISSN: 25766422 NLM ISO Abbreviation: ACS Appl Bio Mater Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Washington, DC : ACS Publications, [2018]- |
| Ιατρικοί όροι (MeSH): | RNA, Double-Stranded*/chemistry , RNA, Double-Stranded*/pharmacology , Acaricides*/pharmacology , Acaricides*/chemistry , Mineral Oil*/chemistry , Mineral Oil*/pharmacology, Animals ; Particle Size ; Materials Testing |
| Περίληψη: | RNA interference (RNAi)-based biopesticides offer precise pest control with minimal environmental impact, yet their field efficacy is limited by rapid degradation of double-stranded RNA (dsRNA) due to UV exposure, nucleases, and poor foliar adhesion. To address these challenges, we developed a mineral oil-based water-in-oil (W/O) emulsion to encapsulate dsRNA targeting the Amphitetranychus viennensis V-ATPase A gene. The formulation was optimized through hydrophilic-lipophilic balance (HLB) screening (optimal HLB = 10), ternary phase ratio adjustment (oil:water:surfactant = 64:20:16), and dsRNA loading concentration tests (optimal: 5000 mg/L). Bioassays assessed toxicity against eggs, nymphs, and adults of Amphitetranychus viennensis, alongside field trials comparing dsRNA@W/O (750× dilution) with naked dsRNA, double-applied naked dsRNA, and the chemical control etoxazole. Key findings demonstrated that dsRNA@W/O significantly enhanced stability: after 72 h of UV/air exposure, 93.67% of activity was retained, compared to complete degradation of naked dsRNA. The formulation accelerated lethality, reducing median lethal time (LT |
| Contributed Indexing: | Keywords: Amphitetranychus viennensis; RNA interference; median lethal time; mineral oil encapsulation; water-in-oil emulsion |
| Substance Nomenclature: | 0 (RNA, Double-Stranded) 0 (Acaricides) 8020-83-5 (Mineral Oil) |
| Entry Date(s): | Date Created: 20251114 Date Completed: 20251215 Latest Revision: 20251215 |
| Update Code: | 20260130 |
| DOI: | 10.1021/acsabm.5c01852 |
| PMID: | 41236780 |
| Βάση Δεδομένων: | MEDLINE |
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