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Mineral Oil Encapsulation Enhances dsRNA-Based Acaricidal Activity against Amphitetranychus viennensis.

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Τίτλος: Mineral Oil Encapsulation Enhances dsRNA-Based Acaricidal Activity against Amphitetranychus viennensis.
Συγγραφείς: Zhang P; Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China.; College of Plant Protection, Shanxi Agricultural University, Taiyuan, Shanxi 030031, China., Zhang G; College of Plant Protection, Shanxi Agricultural University, Taiyuan, Shanxi 030031, China., He L; College of Plant Protection, Shanxi Agricultural University, Taiyuan, Shanxi 030031, China., Liu Z; College of Plant Protection, Shanxi Agricultural University, Taiyuan, Shanxi 030031, China., Yang J; College of Plant Protection, Shanxi Agricultural University, Taiyuan, Shanxi 030031, China., Gao Y; College of Plant Protection, Shanxi Agricultural University, Taiyuan, Shanxi 030031, China., Fan R; College of Plant Protection, Shanxi Agricultural University, Taiyuan, Shanxi 030031, China., Du F; Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China.
Πηγή: ACS applied bio materials [ACS Appl Bio Mater] 2025 Dec 15; Vol. 8 (12), pp. 11155-11166. Date of Electronic Publication: 2025 Nov 14.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: ACS Publications Country of Publication: United States NLM ID: 101729147 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2576-6422 (Electronic) Linking ISSN: 25766422 NLM ISO Abbreviation: ACS Appl Bio Mater Subsets: MEDLINE
Imprint Name(s): Original Publication: Washington, DC : ACS Publications, [2018]-
Ιατρικοί όροι (MeSH): RNA, Double-Stranded*/chemistry , RNA, Double-Stranded*/pharmacology , Acaricides*/pharmacology , Acaricides*/chemistry , Mineral Oil*/chemistry , Mineral Oil*/pharmacology, Animals ; Particle Size ; Materials Testing
Περίληψη: RNA interference (RNAi)-based biopesticides offer precise pest control with minimal environmental impact, yet their field efficacy is limited by rapid degradation of double-stranded RNA (dsRNA) due to UV exposure, nucleases, and poor foliar adhesion. To address these challenges, we developed a mineral oil-based water-in-oil (W/O) emulsion to encapsulate dsRNA targeting the Amphitetranychus viennensis V-ATPase A gene. The formulation was optimized through hydrophilic-lipophilic balance (HLB) screening (optimal HLB = 10), ternary phase ratio adjustment (oil:water:surfactant = 64:20:16), and dsRNA loading concentration tests (optimal: 5000 mg/L). Bioassays assessed toxicity against eggs, nymphs, and adults of Amphitetranychus viennensis, alongside field trials comparing dsRNA@W/O (750× dilution) with naked dsRNA, double-applied naked dsRNA, and the chemical control etoxazole. Key findings demonstrated that dsRNA@W/O significantly enhanced stability: after 72 h of UV/air exposure, 93.67% of activity was retained, compared to complete degradation of naked dsRNA. The formulation accelerated lethality, reducing median lethal time (LT50) from 4.84 to 1.95 days for nymphs and from 4.82 to 2.65 days for adults. Field efficacy at 20 days post-treatment reached 85.75% at 1.33 mg/L dsRNA, outperforming naked dsRNA (62.79%) and approaching etoxazole (95.69%), while using one-third the active ingredient of conventional dsRNA treatments. This work demonstrates a cost-effective, scalable mineral oil encapsulation strategy that synergizes RNAi-mediated pest control with mineral oil's physical effects, offering a sustainable, environmentally safe, and economically feasible pest management approach.
Contributed Indexing: Keywords: Amphitetranychus viennensis; RNA interference; median lethal time; mineral oil encapsulation; water-in-oil emulsion
Substance Nomenclature: 0 (RNA, Double-Stranded)
0 (Acaricides)
8020-83-5 (Mineral Oil)
Entry Date(s): Date Created: 20251114 Date Completed: 20251215 Latest Revision: 20251215
Update Code: 20260130
DOI: 10.1021/acsabm.5c01852
PMID: 41236780
Βάση Δεδομένων: MEDLINE
FullText Links:
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An: 41236780
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PubType: Academic Journal
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Items – Name: Title
  Label: Title
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  Data: Mineral Oil Encapsulation Enhances dsRNA-Based Acaricidal Activity against Amphitetranychus viennensis.
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  Data: <searchLink fieldCode="AU" term="%22Zhang+P%22">Zhang P</searchLink>; Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China.; College of Plant Protection, Shanxi Agricultural University, Taiyuan, Shanxi 030031, China.<br /><searchLink fieldCode="AU" term="%22Zhang+G%22">Zhang G</searchLink>; College of Plant Protection, Shanxi Agricultural University, Taiyuan, Shanxi 030031, China.<br /><searchLink fieldCode="AU" term="%22He+L%22">He L</searchLink>; College of Plant Protection, Shanxi Agricultural University, Taiyuan, Shanxi 030031, China.<br /><searchLink fieldCode="AU" term="%22Liu+Z%22">Liu Z</searchLink>; College of Plant Protection, Shanxi Agricultural University, Taiyuan, Shanxi 030031, China.<br /><searchLink fieldCode="AU" term="%22Yang+J%22">Yang J</searchLink>; College of Plant Protection, Shanxi Agricultural University, Taiyuan, Shanxi 030031, China.<br /><searchLink fieldCode="AU" term="%22Gao+Y%22">Gao Y</searchLink>; College of Plant Protection, Shanxi Agricultural University, Taiyuan, Shanxi 030031, China.<br /><searchLink fieldCode="AU" term="%22Fan+R%22">Fan R</searchLink>; College of Plant Protection, Shanxi Agricultural University, Taiyuan, Shanxi 030031, China.<br /><searchLink fieldCode="AU" term="%22Du+F%22">Du F</searchLink>; Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China.
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  Data: <searchLink fieldCode="JN" term="%22101729147%22">ACS applied bio materials</searchLink> [ACS Appl Bio Mater] 2025 Dec 15; Vol. 8 (12), pp. 11155-11166. <i>Date of Electronic Publication: </i>2025 Nov 14.
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  Data: Journal Article
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  Data: English
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22ACS+Publications%22">ACS Publications </searchLink><i>Country of Publication: </i>United States <i>NLM ID: </i>101729147 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>2576-6422 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2225766422%22">25766422 </searchLink><i>NLM ISO Abbreviation: </i>ACS Appl Bio Mater <i>Subsets: </i>MEDLINE
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  Data: <i>Original Publication</i>: Washington, DC : ACS Publications, [2018]-
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  Data: <searchLink fieldCode="MM" term="%22RNA%2C+Double-Stranded%22">RNA, Double-Stranded*</searchLink>/<searchLink fieldCode="MM" term="%22RNA%2C+Double-Stranded+chemistry%22">chemistry</searchLink> <br /><searchLink fieldCode="MM" term="%22RNA%2C+Double-Stranded%22">RNA, Double-Stranded*</searchLink>/<searchLink fieldCode="MM" term="%22RNA%2C+Double-Stranded+pharmacology%22">pharmacology</searchLink> <br /><searchLink fieldCode="MM" term="%22Acaricides%22">Acaricides*</searchLink>/<searchLink fieldCode="MM" term="%22Acaricides+pharmacology%22">pharmacology</searchLink> <br /><searchLink fieldCode="MM" term="%22Acaricides%22">Acaricides*</searchLink>/<searchLink fieldCode="MM" term="%22Acaricides+chemistry%22">chemistry</searchLink> <br /><searchLink fieldCode="MM" term="%22Mineral+Oil%22">Mineral Oil*</searchLink>/<searchLink fieldCode="MM" term="%22Mineral+Oil+chemistry%22">chemistry</searchLink> <br /><searchLink fieldCode="MM" term="%22Mineral+Oil%22">Mineral Oil*</searchLink>/<searchLink fieldCode="MM" term="%22Mineral+Oil+pharmacology%22">pharmacology</searchLink><br /><searchLink fieldCode="MH" term="%22Animals%22">Animals</searchLink> ; <searchLink fieldCode="MH" term="%22Particle+Size%22">Particle Size</searchLink> ; <searchLink fieldCode="MH" term="%22Materials+Testing%22">Materials Testing</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: RNA interference (RNAi)-based biopesticides offer precise pest control with minimal environmental impact, yet their field efficacy is limited by rapid degradation of double-stranded RNA (dsRNA) due to UV exposure, nucleases, and poor foliar adhesion. To address these challenges, we developed a mineral oil-based water-in-oil (W/O) emulsion to encapsulate dsRNA targeting the Amphitetranychus viennensis V-ATPase A gene. The formulation was optimized through hydrophilic-lipophilic balance (HLB) screening (optimal HLB = 10), ternary phase ratio adjustment (oil:water:surfactant = 64:20:16), and dsRNA loading concentration tests (optimal: 5000 mg/L). Bioassays assessed toxicity against eggs, nymphs, and adults of Amphitetranychus viennensis, alongside field trials comparing dsRNA@W/O (750× dilution) with naked dsRNA, double-applied naked dsRNA, and the chemical control etoxazole. Key findings demonstrated that dsRNA@W/O significantly enhanced stability: after 72 h of UV/air exposure, 93.67% of activity was retained, compared to complete degradation of naked dsRNA. The formulation accelerated lethality, reducing median lethal time (LT<subscript>50</subscript>) from 4.84 to 1.95 days for nymphs and from 4.82 to 2.65 days for adults. Field efficacy at 20 days post-treatment reached 85.75% at 1.33 mg/L dsRNA, outperforming naked dsRNA (62.79%) and approaching etoxazole (95.69%), while using one-third the active ingredient of conventional dsRNA treatments. This work demonstrates a cost-effective, scalable mineral oil encapsulation strategy that synergizes RNAi-mediated pest control with mineral oil's physical effects, offering a sustainable, environmentally safe, and economically feasible pest management approach.
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  Label: Contributed Indexing
  Group:
  Data: <i>Keywords: </i>Amphitetranychus viennensis; RNA interference; median lethal time; mineral oil encapsulation; water-in-oil emulsion
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  Data: 0 (RNA, Double-Stranded)<br />0 (Acaricides)<br />8020-83-5 (Mineral Oil)
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  Data: <i>Date Created: </i>20251114 <i>Date Completed: </i>20251215 <i>Latest Revision: </i>20251215
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  Data: 10.1021/acsabm.5c01852
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  Data: 41236780
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cmedm&AN=41236780
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        Value: 10.1021/acsabm.5c01852
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      – Code: eng
        Text: English
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        StartPage: 11155
    Subjects:
      – SubjectFull: Animals
        Type: general
      – SubjectFull: Particle Size
        Type: general
      – SubjectFull: Materials Testing
        Type: general
      – SubjectFull: RNA, Double-Stranded chemistry
        Type: general
      – SubjectFull: RNA, Double-Stranded pharmacology
        Type: general
      – SubjectFull: Acaricides pharmacology
        Type: general
      – SubjectFull: Acaricides chemistry
        Type: general
      – SubjectFull: Mineral Oil chemistry
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      – SubjectFull: Mineral Oil pharmacology
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      – TitleFull: Mineral Oil Encapsulation Enhances dsRNA-Based Acaricidal Activity against Amphitetranychus viennensis.
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              Text: 2025 Dec 15
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