Academic Journal
Age-related changes in adrenergic regulation of contractility and redox status of glycolytic and oxidative skeletal muscles.
| Τίτλος: | Age-related changes in adrenergic regulation of contractility and redox status of glycolytic and oxidative skeletal muscles. |
|---|---|
| Συγγραφείς: | Odnoshivkina JG; Laboratory of Biophysics of Synaptic Processes, Federal Research Center, Kazan Institute of Biochemistry and Biophysics, 'Kazan Scientific Center of RAS', 2/31 Lobachevsky Street, Box 30, Kazan, 420111, Russia.; Department of Normal Physiology, Institute of Neuroscience, Kazan State Medical University, 49 Butlerova Street, Kazan, 420012, Russia., Sibgatullina GV; Laboratory of Biophysics of Synaptic Processes, Federal Research Center, Kazan Institute of Biochemistry and Biophysics, 'Kazan Scientific Center of RAS', 2/31 Lobachevsky Street, Box 30, Kazan, 420111, Russia., Dmitrieva SA; Laboratory of Biophysics of Synaptic Processes, Federal Research Center, Kazan Institute of Biochemistry and Biophysics, 'Kazan Scientific Center of RAS', 2/31 Lobachevsky Street, Box 30, Kazan, 420111, Russia., Ponomareva AA; Laboratory of Biophysics of Synaptic Processes, Federal Research Center, Kazan Institute of Biochemistry and Biophysics, 'Kazan Scientific Center of RAS', 2/31 Lobachevsky Street, Box 30, Kazan, 420111, Russia., Khuzakhmetova VF; Laboratory of Biophysics of Synaptic Processes, Federal Research Center, Kazan Institute of Biochemistry and Biophysics, 'Kazan Scientific Center of RAS', 2/31 Lobachevsky Street, Box 30, Kazan, 420111, Russia., Bukharaeva EA; Laboratory of Biophysics of Synaptic Processes, Federal Research Center, Kazan Institute of Biochemistry and Biophysics, 'Kazan Scientific Center of RAS', 2/31 Lobachevsky Street, Box 30, Kazan, 420111, Russia., Petrov AM; Laboratory of Biophysics of Synaptic Processes, Federal Research Center, Kazan Institute of Biochemistry and Biophysics, 'Kazan Scientific Center of RAS', 2/31 Lobachevsky Street, Box 30, Kazan, 420111, Russia. alexey.petrov@kazangmu.ru.; Department of Normal Physiology, Institute of Neuroscience, Kazan State Medical University, 49 Butlerova Street, Kazan, 420012, Russia. alexey.petrov@kazangmu.ru. |
| Πηγή: | GeroScience [Geroscience] 2026 Aug; Vol. 48 (4), pp. 5957-5986. Date of Electronic Publication: 2025 Nov 13. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Springer International Publishing Country of Publication: Switzerland NLM ID: 101686284 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2509-2723 (Electronic) Linking ISSN: 25092723 NLM ISO Abbreviation: Geroscience Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Cham : Springer International Publishing, [2017]- |
| Ιατρικοί όροι (MeSH): | Muscle, Skeletal*/metabolism , Muscle, Skeletal*/drug effects , Muscle, Skeletal*/physiology , Muscle Contraction*/physiology , Muscle Contraction*/drug effects , Aging*/physiology , Oxidative Stress*/physiology , Glycolysis*/physiology, Epinephrine/pharmacology ; Fenoterol/pharmacology ; Animals ; Mice ; Oxidation-Reduction ; Male ; Mice, Inbred C57BL |
| Περίληψη: | Aging is associated with skeletal muscle weakness and oxidative stress. Here, we hypothesized that aging may lead to alterations in adrenoceptor (AR)-dependent regulation of muscle contractility. These changes can aggravate muscle contractile dysfunction and correlate with redox disturbances. Glycolytic extensor digitorum longus (EDL) and oxidative soleus muscles from young adult (3 months), middle-aged (12 months), and old (24 months) mice were used. Contractile responses were elicited by electrical stimulation at different frequencies. During the aging process, inotropic effects of adrenaline in both muscles changed in a similar manner. Adrenaline caused a positive inotropic effect in young adult mice, then its action disappeared at middle age, and, finally, adrenaline decreased the contraction force at old age. Fenoterol, a β-AR agonist, exerted a negative inotropic action in EDL and soleus muscles of young adult and middle-aged mice. This effect of fenoterol is partially preserved in soleus (but not EDL) muscles at advanced age. In both muscles, the positive inotropy of adrenaline was blocked by β2-AR antagonist, whereas the fenoterol-mediated suppression of contraction force was inhibited by β3-AR antagonists. Immunofluorescent labeling revealed both β2- and β3-AR-positive signals at the sarcolemma. Accordingly, the age-related shift of balance from positive to negative inotropic action of adrenaline might be partially dependent on the interplay of β2- and β3-adrenergic signaling in the skeletal muscles. Despite the commonalities in the changes in adrenergic regulation of contractions in both muscles, oxidative stress, decline in antioxidant enzyme activities, and ultrastructural disturbances were much more clearly expressed in aged EDL versus soleus muscles. (© 2025. The Author(s), under exclusive licence to American Aging Association.) |
| Competing Interests: | Declarations. Ethics approval: The experimental protocols (Protocols #23–1/23–2/2, #23–4/6, 28 February,2023; #23/7 12 May 2023) were approved by the Bioethics Committee of Kazan Federal Scientific Centre and met the requirements of the EU Directive 2010/63/EU as well as European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes (Council of Europe No 123, Strasbourg, 1985). Consent for Publication: Not applicable. Conflict of interest: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. All authors consent to publish the article. |
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| Grant Information: | 23-15-00124 Russian Science Foundation |
| Contributed Indexing: | Keywords: Adrenaline; Adrenoceptor; Aging; Contraction; Oxidative stress; Sarcopenia; Skeletal muscle |
| Substance Nomenclature: | YKH834O4BH (Epinephrine) 22M9P70OQ9 (Fenoterol) |
| Entry Date(s): | Date Created: 20251113 Date Completed: 20260914 Latest Revision: 20260917 |
| Update Code: | 20260917 |
| PubMed Central ID: | PMC13575024 |
| DOI: | 10.1007/s11357-025-02005-w |
| PMID: | 41231352 |
| Βάση Δεδομένων: | MEDLINE |
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