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Adipocyte Deficiency Promotes Early Development of Mammary Gland Alveolar Epithelial Cell.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Adipocyte Deficiency Promotes Early Development of Mammary Gland Alveolar Epithelial Cell.
Συγγραφείς: Xu J; Nanjing Women and Children's Healthcare Institute, Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, Nanjing, China.; Department of Pediatrics, Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, Nanjing, China., Sun Q; Nanjing Women and Children's Healthcare Institute, Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, Nanjing, China.; Department of Obstetrics and Gynecology, Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, Nanjing, China., Xu L; Nanjing Women and Children's Healthcare Institute, Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, Nanjing, China., Liu L; Department of Obstetrics and Gynecology, Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, Nanjing, China., Liu L; Nanjing Women and Children's Healthcare Institute, Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, Nanjing, China.
Πηγή: FASEB journal : official publication of the Federation of American Societies for Experimental Biology [FASEB J] 2025 Nov 15; Vol. 39 (21), pp. e71219.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Federation of American Societies for Experimental Biology Country of Publication: United States NLM ID: 8804484 Publication Model: Print Cited Medium: Internet ISSN: 1530-6860 (Electronic) Linking ISSN: 08926638 NLM ISO Abbreviation: FASEB J Subsets: MEDLINE
Imprint Name(s): Publication: 2020- : [Bethesda, Md.] : Hoboken, NJ : Federation of American Societies for Experimental Biology ; Wiley
Original Publication: [Bethesda, Md.] : The Federation, [c1987-
Ιατρικοί όροι (MeSH): Adipocytes*/metabolism , Adipocytes*/cytology , Mammary Glands, Animal*/cytology , Mammary Glands, Animal*/metabolism , Mammary Glands, Animal*/growth & development , Alveolar Epithelial Cells*/metabolism , Alveolar Epithelial Cells*/cytology, Cell Differentiation/physiology ; Integrin beta1/metabolism ; STAT5 Transcription Factor/metabolism ; Epithelial Cells/metabolism ; Animals ; Female ; Mice ; Pregnancy ; Lactation ; Apoptosis ; Mice, Transgenic
Περίληψη: Adipocytes undergo dramatic changes during to support the development of the mammary gland. However, their precise role and mechanism of action in pregnancy and lactation remain poorly understood. To explore how adipocyte elimination influences alveolar epithelial cells (AECs) in the mammary gland, we mated mice expressing the diphtheria toxin receptor (iDTR) to those bearing the adiponectin (adipoq) cre. Using this mouse model of inducible and transient adipocyte ablation, we demonstrated that adipocyte loss promotes early development of AECs. During pregnancy, adipocyte loss induces activation of STAT5 through integrin β1/RAC1, which promotes early differentiation of AECs. During lactation, adipocyte loss results in upregulation of TGFβ/WNT5A expression, thereby triggering early apoptosis of AECs. Collectively, our findings reveal an unanticipated, stage-specific regulatory function of adipocytes in orchestrating AECs development during pregnancy and lactation.
(© 2025 Federation of American Societies for Experimental Biology.)
References: J. I. Englund, A. Ritchie, L. Blaas, et al., “Laminin Alpha 5 Regulates Mammary Gland Remodeling Through Luminal Cell Differentiation and Wnt4‐Mediated Epithelial Crosstalk,” Development 148 (2021): dev199281.
O. Cazares, S. Chatterjee, P. Lee, et al., “Alveolar Progenitor Differentiation and Lactation Depends on Paracrine Inhibition of Notch via ROBO1/CTNNB1/JAG1,” Development 148 (2021): dev199940.
C. J. Watson, “Alveolar Cells in the Mammary Gland: Lineage Commitment and Cell Death,” Biochemical Journal 479 (2022): 995–1006.
R. Nightingale, C. M. Reehorst, N. Vukelic, et al., “Ehf Controls Mammary Alveolar Lineage Differentiation and Is a Putative Suppressor of Breast Tumorigenesis,” Developmental Cell 59 (2024): 1988–2004.e1911.
Q. A. Wang and P. E. Scherer, “Remodeling of Murine Mammary Adipose Tissue During Pregnancy, Lactation, and Involution,” Journal of Mammary Gland Biology and Neoplasia 24 (2019): 207–212.
Q. A. Wang, A. Song, W. Chen, et al., “Reversible De‐Differentiation of Mature White Adipocytes Into Preadipocyte‐Like Precursors During Lactation,” Cell Metabolism 28 (2018): 282–288.e283.
J. Sumbal and Z. Koledova, “FGF Signaling in Mammary Gland Fibroblasts Regulates Multiple Fibroblast Functions and Mammary Epithelial Morphogenesis,” Development 146 (2019): dev185306.
G. Rauner and C. Kuperwasser, “Microenvironmental Control of Cell Fate Decisions in Mammary Gland Development and Cancer,” Developmental Cell 56 (2021): 1875–1883.
H. Macias and L. Hinck, “Mammary Gland Development,” Wiley Interdisciplinary Reviews: Developmental Biology 1 (2012): 533–557.
J. L. Inman, C. Robertson, J. D. Mott, and M. J. Bissell, “Mammary Gland Development: Cell Fate Specification, Stem Cells and the Microenvironment,” Development 142 (2015): 1028–1042.
R. C. Hovey and L. Aimo, “Diverse and Active Roles for Adipocytes During Mammary Gland Growth and Function,” Journal of Mammary Gland Biology and Neoplasia 15 (2010): 279–290.
R. K. Zwick, M. C. Rudolph, B. A. Shook, et al., “Adipocyte Hypertrophy and Lipid Dynamics Underlie Mammary Gland Remodeling After Lactation,” Nature Communications 9 (2018): 3592.
M. Morroni, A. Giordano, M. C. Zingaretti, et al., “Reversible Transdifferentiation of Secretory Epithelial Cells Into Adipocytes in the Mammary Gland,” Proceedings of the National Academy of Sciences of the United States of America 101 (2004): 16801–16806.
A. Brenot, I. Hutson, and C. Harris, “Epithelial‐Adipocyte Interactions Are Required for Mammary Gland Development, but Not for Milk Production or Fertility,” Developmental Biology 458 (2020): 153–163.
S. Landskroner‐Eiger, J. Park, D. Israel, J. W. Pollard, and P. E. Scherer, “Morphogenesis of the Developing Mammary Gland: Stage‐Dependent Impact of Adipocytes,” Developmental Biology 344 (2010): 968–978.
H. Yuan, X. Wang, J. Lu, et al., “MMTV‐NeuT/ATTAC Mice: A New Model for Studying the Stromal Tumor Microenvironment,” Oncotarget 9 (2018): 8042–8053.
F. Wang, S. E. Mullican, J. R. DiSpirito, L. C. Peed, and M. A. Lazar, “Lipoatrophy and Severe Metabolic Disturbance in Mice With Fat‐Specific Deletion of PPARgamma,” Proceedings of the National Academy of Sciences of the United States of America 110 (2013): 18656–18661.
F. M. Hannan, T. Elajnaf, L. N. Vandenberg, S. H. Kennedy, and R. V. Thakker, “Hormonal Regulation of Mammary Gland Development and Lactation,” Nature Reviews. Endocrinology 19 (2023): 46–61.
Ö. Karayazi Atıcı, N. Govindrajan, I. Lopetegui‐González, and C. S. Shemanko, “Prolactin: A Hormone With Diverse Functions From Mammary Gland Development to Cancer Metastasis,” Seminars in Cell & Developmental Biology 114 (2021): 159–170.
P. F. Slepicka, A. V. H. Somasundara, and C. O. Dos Santos, “The Molecular Basis of Mammary Gland Development and Epithelial Differentiation,” Seminars in Cell & Developmental Biology 114 (2021): 93–112.
K. Willingham, E. McNulty, K. Anderson, J. Hayes‐Klug, A. Nalls, and C. Mathiason, “Milk Collection Methods for Mice and Reeves' Muntjac Deer,” Journal of Visualized Experiments 19, no. 89 (2014): 51007.
Y. Lu, T. Zhou, C. Xu, et al., “Occludin Is a Target of Src Kinase and Promotes Lipid Secretion by Binding to BTN1a1 and XOR,” PLoS Biology 20 (2022): e3001518.
T. Buch, F. L. Heppner, C. Tertilt, et al., “A Cre‐Inducible Diphtheria Toxin Receptor Mediates Cell Lineage Ablation After Toxin Administration,” Nature Methods 2 (2005): 419–426.
W. Zou, N. Rohatgi, J. R. Brestoff, et al., “Ablation of Fat Cells in Adult Mice Induces Massive Bone Gain,” Cell Metabolism 32 (2020): 801–813.e806.
T. D. Challa, D. H. Dapito, E. Kulenkampff, et al., “A Genetic Model to Study the Contribution of Brown and Brite Adipocytes to Metabolism,” Cell Reports 30 (2020): 3424–3433.e3424.
J. Zhou, R. Chehab, J. Tkalcevic, et al., “Elf5 Is Essential for Early Embryogenesis and Mammary Gland Development During Pregnancy and Lactation,” EMBO Journal 24 (2005): 635–644.
S. Hinz, A. Manousopoulou, M. Miyano, et al., “Deep Proteome Profiling of Human Mammary Epithelia at Lineage and Age Resolution,” iScience 24 (2021): 103026.
M. J. Naylor, N. Li, J. Cheung, et al., “Ablation of beta1 Integrin in Mammary Epithelium Reveals a Key Role for Integrin in Glandular Morphogenesis and Differentiation,” Journal of Cell Biology 171 (2005): 717–728.
N. Akhtar and C. H. Streuli, “Rac1 Links Integrin‐Mediated Adhesion to the Control of Lactational Differentiation in Mammary Epithelia,” Journal of Cell Biology 173 (2006): 781–793.
Z. Salemi, R. Azizi, F. Fallahian, and M. Aghaei, “Integrin α2β1 Inhibition Attenuates Prostate Cancer Cell Proliferation by Cell Cycle Arrest, Promoting Apoptosis and Reducing Epithelial‐Mesenchymal Transition,” Journal of Cellular Physiology 236 (2021): 4954–4965.
X. Wang, F. Li, L. Xie, et al., “Inhibition of Overactive TGF‐β Attenuates Progression of Heterotopic Ossification in Mice,” Nature Communications 9 (2018): 551.
R. K. Zwick, C. F. Guerrero‐Juarez, V. Horsley, and M. V. Plikus, “Anatomical, Physiological, and Functional Diversity of Adipose Tissue,” Cell Metabolism 27 (2018): 68–83.
S. Ruijtenberg and S. van den Heuvel, “Coordinating Cell Proliferation and Differentiation: Antagonism Between Cell Cycle Regulators and Cell Type‐Specific Gene Expression,” Cell Cycle 15 (2016): 196–212.
C. J. Watson and P. A. Kreuzaler, “Remodeling Mechanisms of the Mammary Gland During Involution,” International Journal of Developmental Biology 55 (2011): 757–762.
M. Li, X. Liu, G. Robinson, et al., “Mammary‐Derived Signals Activate Programmed Cell Death During the First Stage of Mammary Gland Involution,” Proceedings of the National Academy of Sciences of the United States of America 94 (1997): 3425–3430.
L. Hennighausen and G. W. Robinson, “Signaling Pathways in Mammary Gland Development,” Developmental Cell 1 (2001): 467–475.
N. J. Brady, M. A. Farrar, and K. L. Schwertfeger, “STAT5 Deletion in Macrophages Alters Ductal Elongation and Branching During Mammary Gland Development,” Developmental Biology 428 (2017): 232–244.
S. Haricharan and Y. Li, “STAT Signaling in Mammary Gland Differentiation, Cell Survival and Tumorigenesis,” Molecular and Cellular Endocrinology 382 (2014): 560–569.
M. Tian, Y. Qi, X. Zhang, et al., “Regulation of the JAK2‐STAT5 Pathway by Signaling Molecules in the Mammary Gland,” Frontiers in Cell and Development Biology 8 (2020): 604896.
N. Akhtar, R. Marlow, E. Lambert, et al., “Molecular Dissection of Integrin Signalling Proteins in the Control of Mammary Epithelial Development and Differentiation,” Development 136 (2009): 1019–1027.
K. B. Ewan, G. Shyamala, S. A. Ravani, et al., “Latent Transforming Growth Factor‐Beta Activation in Mammary Gland: Regulation by Ovarian Hormones Affects Ductal and Alveolar Proliferation,” American Journal of Pathology 160 (2002): 2081–2093.
K. Roarty and R. Serra, “Wnt5a Is Required for Proper Mammary Gland Development and TGF‐Beta‐Mediated Inhibition of Ductal Growth,” Development 134 (2007): 3929–3939.
Grant Information: 82301824 MOST | National Natural Science Foundation of China (NSFC); 82401893 MOST | National Natural Science Foundation of China (NSFC)
Contributed Indexing: Keywords: adipocyte; alveolar epithelial cell; development; lactation; mammary gland
Substance Nomenclature: 0 (Integrin beta1)
0 (STAT5 Transcription Factor)
Entry Date(s): Date Created: 20251104 Date Completed: 20251104 Latest Revision: 20260216
Update Code: 20260216
DOI: 10.1096/fj.202503289R
PMID: 41186192
Βάση Δεδομένων: MEDLINE
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Items – Name: Title
  Label: Title
  Group: Ti
  Data: Adipocyte Deficiency Promotes Early Development of Mammary Gland Alveolar Epithelial Cell.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AU" term="%22Xu+J%22">Xu J</searchLink>; Nanjing Women and Children's Healthcare Institute, Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, Nanjing, China.; Department of Pediatrics, Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, Nanjing, China.<br /><searchLink fieldCode="AU" term="%22Sun+Q%22">Sun Q</searchLink>; Nanjing Women and Children's Healthcare Institute, Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, Nanjing, China.; Department of Obstetrics and Gynecology, Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, Nanjing, China.<br /><searchLink fieldCode="AU" term="%22Xu+L%22">Xu L</searchLink>; Nanjing Women and Children's Healthcare Institute, Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, Nanjing, China.<br /><searchLink fieldCode="AU" term="%22Liu+L%22">Liu L</searchLink>; Department of Obstetrics and Gynecology, Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, Nanjing, China.<br /><searchLink fieldCode="AU" term="%22Liu+L%22">Liu L</searchLink>; Nanjing Women and Children's Healthcare Institute, Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, Nanjing, China.
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  Data: <searchLink fieldCode="JN" term="%228804484%22">FASEB journal : official publication of the Federation of American Societies for Experimental Biology</searchLink> [FASEB J] 2025 Nov 15; Vol. 39 (21), pp. e71219.
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  Data: English
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  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22Federation+of+American+Societies+for+Experimental+Biology%22">Federation of American Societies for Experimental Biology </searchLink><i>Country of Publication: </i>United States <i>NLM ID: </i>8804484 <i>Publication Model: </i>Print <i>Cited Medium: </i>Internet <i>ISSN: </i>1530-6860 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2208926638%22">08926638 </searchLink><i>NLM ISO Abbreviation: </i>FASEB J <i>Subsets: </i>MEDLINE
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  Data: <i>Publication</i>: 2020- : [Bethesda, Md.] : Hoboken, NJ : Federation of American Societies for Experimental Biology ; Wiley<br /><i>Original Publication</i>: [Bethesda, Md.] : The Federation, [c1987-
– Name: SubjectMESH
  Label: MeSH Terms
  Group: Su
  Data: <searchLink fieldCode="MM" term="%22Adipocytes%22">Adipocytes*</searchLink>/<searchLink fieldCode="MM" term="%22Adipocytes+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Adipocytes%22">Adipocytes*</searchLink>/<searchLink fieldCode="MM" term="%22Adipocytes+cytology%22">cytology</searchLink> <br /><searchLink fieldCode="MM" term="%22Mammary+Glands%2C+Animal%22">Mammary Glands, Animal*</searchLink>/<searchLink fieldCode="MM" term="%22Mammary+Glands%2C+Animal+cytology%22">cytology</searchLink> <br /><searchLink fieldCode="MM" term="%22Mammary+Glands%2C+Animal%22">Mammary Glands, Animal*</searchLink>/<searchLink fieldCode="MM" term="%22Mammary+Glands%2C+Animal+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Mammary+Glands%2C+Animal%22">Mammary Glands, Animal*</searchLink>/<searchLink fieldCode="MM" term="%22Mammary+Glands%2C+Animal+growth+%26+development%22">growth & development</searchLink> <br /><searchLink fieldCode="MM" term="%22Alveolar+Epithelial+Cells%22">Alveolar Epithelial Cells*</searchLink>/<searchLink fieldCode="MM" term="%22Alveolar+Epithelial+Cells+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Alveolar+Epithelial+Cells%22">Alveolar Epithelial Cells*</searchLink>/<searchLink fieldCode="MM" term="%22Alveolar+Epithelial+Cells+cytology%22">cytology</searchLink><br /><searchLink fieldCode="MH" term="%22Cell+Differentiation%22">Cell Differentiation</searchLink>/<searchLink fieldCode="MH" term="%22Cell+Differentiation+physiology%22">physiology</searchLink> ; <searchLink fieldCode="MH" term="%22Integrin+beta1%22">Integrin beta1</searchLink>/<searchLink fieldCode="MH" term="%22Integrin+beta1+metabolism%22">metabolism</searchLink> ; <searchLink fieldCode="MH" term="%22STAT5+Transcription+Factor%22">STAT5 Transcription Factor</searchLink>/<searchLink fieldCode="MH" term="%22STAT5+Transcription+Factor+metabolism%22">metabolism</searchLink> ; <searchLink fieldCode="MH" term="%22Epithelial+Cells%22">Epithelial Cells</searchLink>/<searchLink fieldCode="MH" term="%22Epithelial+Cells+metabolism%22">metabolism</searchLink> ; <searchLink fieldCode="MH" term="%22Animals%22">Animals</searchLink> ; <searchLink fieldCode="MH" term="%22Female%22">Female</searchLink> ; <searchLink fieldCode="MH" term="%22Mice%22">Mice</searchLink> ; <searchLink fieldCode="MH" term="%22Pregnancy%22">Pregnancy</searchLink> ; <searchLink fieldCode="MH" term="%22Lactation%22">Lactation</searchLink> ; <searchLink fieldCode="MH" term="%22Apoptosis%22">Apoptosis</searchLink> ; <searchLink fieldCode="MH" term="%22Mice%2C+Transgenic%22">Mice, Transgenic</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Adipocytes undergo dramatic changes during to support the development of the mammary gland. However, their precise role and mechanism of action in pregnancy and lactation remain poorly understood. To explore how adipocyte elimination influences alveolar epithelial cells (AECs) in the mammary gland, we mated mice expressing the diphtheria toxin receptor (iDTR) to those bearing the adiponectin (adipoq) cre. Using this mouse model of inducible and transient adipocyte ablation, we demonstrated that adipocyte loss promotes early development of AECs. During pregnancy, adipocyte loss induces activation of STAT5 through integrin β1/RAC1, which promotes early differentiation of AECs. During lactation, adipocyte loss results in upregulation of TGFβ/WNT5A expression, thereby triggering early apoptosis of AECs. Collectively, our findings reveal an unanticipated, stage-specific regulatory function of adipocytes in orchestrating AECs development during pregnancy and lactation.<br /> (© 2025 Federation of American Societies for Experimental Biology.)
– Name: Ref
  Label: References
  Group: RefInfo
  Data: J. I. Englund, A. Ritchie, L. Blaas, et al., “Laminin Alpha 5 Regulates Mammary Gland Remodeling Through Luminal Cell Differentiation and Wnt4‐Mediated Epithelial Crosstalk,” Development 148 (2021): dev199281.<br />O. Cazares, S. Chatterjee, P. Lee, et al., “Alveolar Progenitor Differentiation and Lactation Depends on Paracrine Inhibition of Notch via ROBO1/CTNNB1/JAG1,” Development 148 (2021): dev199940.<br />C. J. Watson, “Alveolar Cells in the Mammary Gland: Lineage Commitment and Cell Death,” Biochemical Journal 479 (2022): 995–1006.<br />R. Nightingale, C. M. Reehorst, N. Vukelic, et al., “Ehf Controls Mammary Alveolar Lineage Differentiation and Is a Putative Suppressor of Breast Tumorigenesis,” Developmental Cell 59 (2024): 1988–2004.e1911.<br />Q. A. Wang and P. E. Scherer, “Remodeling of Murine Mammary Adipose Tissue During Pregnancy, Lactation, and Involution,” Journal of Mammary Gland Biology and Neoplasia 24 (2019): 207–212.<br />Q. A. Wang, A. Song, W. Chen, et al., “Reversible De‐Differentiation of Mature White Adipocytes Into Preadipocyte‐Like Precursors During Lactation,” Cell Metabolism 28 (2018): 282–288.e283.<br />J. Sumbal and Z. Koledova, “FGF Signaling in Mammary Gland Fibroblasts Regulates Multiple Fibroblast Functions and Mammary Epithelial Morphogenesis,” Development 146 (2019): dev185306.<br />G. Rauner and C. Kuperwasser, “Microenvironmental Control of Cell Fate Decisions in Mammary Gland Development and Cancer,” Developmental Cell 56 (2021): 1875–1883.<br />H. Macias and L. Hinck, “Mammary Gland Development,” Wiley Interdisciplinary Reviews: Developmental Biology 1 (2012): 533–557.<br />J. L. Inman, C. Robertson, J. D. Mott, and M. J. Bissell, “Mammary Gland Development: Cell Fate Specification, Stem Cells and the Microenvironment,” Development 142 (2015): 1028–1042.<br />R. C. Hovey and L. Aimo, “Diverse and Active Roles for Adipocytes During Mammary Gland Growth and Function,” Journal of Mammary Gland Biology and Neoplasia 15 (2010): 279–290.<br />R. K. Zwick, M. C. Rudolph, B. A. Shook, et al., “Adipocyte Hypertrophy and Lipid Dynamics Underlie Mammary Gland Remodeling After Lactation,” Nature Communications 9 (2018): 3592.<br />M. Morroni, A. Giordano, M. C. Zingaretti, et al., “Reversible Transdifferentiation of Secretory Epithelial Cells Into Adipocytes in the Mammary Gland,” Proceedings of the National Academy of Sciences of the United States of America 101 (2004): 16801–16806.<br />A. Brenot, I. Hutson, and C. Harris, “Epithelial‐Adipocyte Interactions Are Required for Mammary Gland Development, but Not for Milk Production or Fertility,” Developmental Biology 458 (2020): 153–163.<br />S. Landskroner‐Eiger, J. Park, D. Israel, J. W. Pollard, and P. E. Scherer, “Morphogenesis of the Developing Mammary Gland: Stage‐Dependent Impact of Adipocytes,” Developmental Biology 344 (2010): 968–978.<br />H. Yuan, X. Wang, J. Lu, et al., “MMTV‐NeuT/ATTAC Mice: A New Model for Studying the Stromal Tumor Microenvironment,” Oncotarget 9 (2018): 8042–8053.<br />F. Wang, S. E. Mullican, J. R. DiSpirito, L. C. Peed, and M. A. Lazar, “Lipoatrophy and Severe Metabolic Disturbance in Mice With Fat‐Specific Deletion of PPARgamma,” Proceedings of the National Academy of Sciences of the United States of America 110 (2013): 18656–18661.<br />F. M. Hannan, T. Elajnaf, L. N. Vandenberg, S. H. Kennedy, and R. V. Thakker, “Hormonal Regulation of Mammary Gland Development and Lactation,” Nature Reviews. Endocrinology 19 (2023): 46–61.<br />Ö. Karayazi Atıcı, N. Govindrajan, I. Lopetegui‐González, and C. S. Shemanko, “Prolactin: A Hormone With Diverse Functions From Mammary Gland Development to Cancer Metastasis,” Seminars in Cell & Developmental Biology 114 (2021): 159–170.<br />P. F. Slepicka, A. V. H. Somasundara, and C. O. Dos Santos, “The Molecular Basis of Mammary Gland Development and Epithelial Differentiation,” Seminars in Cell & Developmental Biology 114 (2021): 93–112.<br />K. Willingham, E. McNulty, K. Anderson, J. Hayes‐Klug, A. Nalls, and C. Mathiason, “Milk Collection Methods for Mice and Reeves' Muntjac Deer,” Journal of Visualized Experiments 19, no. 89 (2014): 51007.<br />Y. Lu, T. Zhou, C. Xu, et al., “Occludin Is a Target of Src Kinase and Promotes Lipid Secretion by Binding to BTN1a1 and XOR,” PLoS Biology 20 (2022): e3001518.<br />T. Buch, F. L. Heppner, C. Tertilt, et al., “A Cre‐Inducible Diphtheria Toxin Receptor Mediates Cell Lineage Ablation After Toxin Administration,” Nature Methods 2 (2005): 419–426.<br />W. Zou, N. Rohatgi, J. R. Brestoff, et al., “Ablation of Fat Cells in Adult Mice Induces Massive Bone Gain,” Cell Metabolism 32 (2020): 801–813.e806.<br />T. D. Challa, D. H. Dapito, E. Kulenkampff, et al., “A Genetic Model to Study the Contribution of Brown and Brite Adipocytes to Metabolism,” Cell Reports 30 (2020): 3424–3433.e3424.<br />J. Zhou, R. Chehab, J. Tkalcevic, et al., “Elf5 Is Essential for Early Embryogenesis and Mammary Gland Development During Pregnancy and Lactation,” EMBO Journal 24 (2005): 635–644.<br />S. Hinz, A. Manousopoulou, M. Miyano, et al., “Deep Proteome Profiling of Human Mammary Epithelia at Lineage and Age Resolution,” iScience 24 (2021): 103026.<br />M. J. Naylor, N. Li, J. Cheung, et al., “Ablation of beta1 Integrin in Mammary Epithelium Reveals a Key Role for Integrin in Glandular Morphogenesis and Differentiation,” Journal of Cell Biology 171 (2005): 717–728.<br />N. Akhtar and C. H. Streuli, “Rac1 Links Integrin‐Mediated Adhesion to the Control of Lactational Differentiation in Mammary Epithelia,” Journal of Cell Biology 173 (2006): 781–793.<br />Z. Salemi, R. Azizi, F. Fallahian, and M. Aghaei, “Integrin α2β1 Inhibition Attenuates Prostate Cancer Cell Proliferation by Cell Cycle Arrest, Promoting Apoptosis and Reducing Epithelial‐Mesenchymal Transition,” Journal of Cellular Physiology 236 (2021): 4954–4965.<br />X. Wang, F. Li, L. Xie, et al., “Inhibition of Overactive TGF‐β Attenuates Progression of Heterotopic Ossification in Mice,” Nature Communications 9 (2018): 551.<br />R. K. Zwick, C. F. Guerrero‐Juarez, V. Horsley, and M. V. Plikus, “Anatomical, Physiological, and Functional Diversity of Adipose Tissue,” Cell Metabolism 27 (2018): 68–83.<br />S. Ruijtenberg and S. van den Heuvel, “Coordinating Cell Proliferation and Differentiation: Antagonism Between Cell Cycle Regulators and Cell Type‐Specific Gene Expression,” Cell Cycle 15 (2016): 196–212.<br />C. J. Watson and P. A. Kreuzaler, “Remodeling Mechanisms of the Mammary Gland During Involution,” International Journal of Developmental Biology 55 (2011): 757–762.<br />M. Li, X. Liu, G. Robinson, et al., “Mammary‐Derived Signals Activate Programmed Cell Death During the First Stage of Mammary Gland Involution,” Proceedings of the National Academy of Sciences of the United States of America 94 (1997): 3425–3430.<br />L. Hennighausen and G. W. Robinson, “Signaling Pathways in Mammary Gland Development,” Developmental Cell 1 (2001): 467–475.<br />N. J. Brady, M. A. Farrar, and K. L. Schwertfeger, “STAT5 Deletion in Macrophages Alters Ductal Elongation and Branching During Mammary Gland Development,” Developmental Biology 428 (2017): 232–244.<br />S. Haricharan and Y. Li, “STAT Signaling in Mammary Gland Differentiation, Cell Survival and Tumorigenesis,” Molecular and Cellular Endocrinology 382 (2014): 560–569.<br />M. Tian, Y. Qi, X. Zhang, et al., “Regulation of the JAK2‐STAT5 Pathway by Signaling Molecules in the Mammary Gland,” Frontiers in Cell and Development Biology 8 (2020): 604896.<br />N. Akhtar, R. Marlow, E. Lambert, et al., “Molecular Dissection of Integrin Signalling Proteins in the Control of Mammary Epithelial Development and Differentiation,” Development 136 (2009): 1019–1027.<br />K. B. Ewan, G. Shyamala, S. A. Ravani, et al., “Latent Transforming Growth Factor‐Beta Activation in Mammary Gland: Regulation by Ovarian Hormones Affects Ductal and Alveolar Proliferation,” American Journal of Pathology 160 (2002): 2081–2093.<br />K. Roarty and R. Serra, “Wnt5a Is Required for Proper Mammary Gland Development and TGF‐Beta‐Mediated Inhibition of Ductal Growth,” Development 134 (2007): 3929–3939.
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  Data: 82301824 MOST | National Natural Science Foundation of China (NSFC); 82401893 MOST | National Natural Science Foundation of China (NSFC)
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  Data: <i>Keywords: </i>adipocyte; alveolar epithelial cell; development; lactation; mammary gland
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        Value: 10.1096/fj.202503289R
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      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        StartPage: e71219
    Subjects:
      – SubjectFull: Cell Differentiation physiology
        Type: general
      – SubjectFull: Integrin beta1 metabolism
        Type: general
      – SubjectFull: STAT5 Transcription Factor metabolism
        Type: general
      – SubjectFull: Epithelial Cells metabolism
        Type: general
      – SubjectFull: Animals
        Type: general
      – SubjectFull: Female
        Type: general
      – SubjectFull: Mice
        Type: general
      – SubjectFull: Pregnancy
        Type: general
      – SubjectFull: Lactation
        Type: general
      – SubjectFull: Apoptosis
        Type: general
      – SubjectFull: Mice, Transgenic
        Type: general
      – SubjectFull: Adipocytes metabolism
        Type: general
      – SubjectFull: Adipocytes cytology
        Type: general
      – SubjectFull: Mammary Glands, Animal cytology
        Type: general
      – SubjectFull: Mammary Glands, Animal metabolism
        Type: general
      – SubjectFull: Mammary Glands, Animal growth & development
        Type: general
      – SubjectFull: Alveolar Epithelial Cells metabolism
        Type: general
      – SubjectFull: Alveolar Epithelial Cells cytology
        Type: general
    Titles:
      – TitleFull: Adipocyte Deficiency Promotes Early Development of Mammary Gland Alveolar Epithelial Cell.
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              Text: 2025 Nov 15
              Type: published
              Y: 2025
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            – Type: issn-electronic
              Value: 1530-6860
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              Value: 39
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            – TitleFull: FASEB journal : official publication of the Federation of American Societies for Experimental Biology
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