Academic Journal
Au@Ag@AgCl core-shell nanostructure-based label-free biosensor for sensitive colorimetric detection of urease.
| Τίτλος: | Au@Ag@AgCl core-shell nanostructure-based label-free biosensor for sensitive colorimetric detection of urease. |
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| Συγγραφείς: | Li MY; State Key Laboratory of Chemical Safety, College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao, 266580, P. R. China., Yu Y; State Key Laboratory of Chemical Safety, College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao, 266580, P. R. China., Zhang RQ; Qingdao Academy of Agricultural Sciences, Qingdao, 266100, China., Qiu Z; State Key Laboratory of Chemical Safety, College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao, 266580, P. R. China., Li X; State Key Laboratory of Chemical Safety, College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao, 266580, P. R. China., Wang YH; State Key Laboratory of Chemical Safety, College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao, 266580, P. R. China., Qi J; State Key Laboratory of Chemical Safety, College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao, 266580, P. R. China., Zeng J; State Key Laboratory of Chemical Safety, College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao, 266580, P. R. China., Wen CY; State Key Laboratory of Chemical Safety, College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao, 266580, P. R. China. flcyxt@163.com. |
| Πηγή: | Mikrochimica acta [Mikrochim Acta] 2025 Nov 01; Vol. 192 (11), pp. 778. Date of Electronic Publication: 2025 Nov 01. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Springer-Verlag Country of Publication: Austria NLM ID: 7808782 Publication Model: Electronic Cited Medium: Internet ISSN: 1436-5073 (Electronic) Linking ISSN: 00263672 NLM ISO Abbreviation: Mikrochim Acta Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Wien ; New York : Springer-Verlag. |
| Ιατρικοί όροι (MeSH): | Urease*/analysis , Urease*/metabolism , Colorimetry*/methods , Biosensing Techniques*/methods , Gold*/chemistry , Metal Nanoparticles*/chemistry , Silver Compounds*/chemistry, Silver/chemistry ; Saliva/chemistry ; Saliva/enzymology ; Limit of Detection ; Humans |
| Περίληψη: | A label-free colorimetric biosensor based on Au@Ag@AgCl core-shell nanomaterials has been developed. The biosensor operated through a unique mechanism where Au@Ag@AgCl nanoparticles were progressively etched by ammonia produced during urease-catalyzed urea decomposition. This etching process induced a distinct color transition of the colloidal solution from orange to pink and ultimately to red, enabling quantitative determination of urease concentration. The multi-core-shell design of Au@Ag@AgCl provided highly enriched color gradients and significantly enhanced visual resolution, achieving a detection limit of 0.147 U/mL, which was 85 times lower than that of the commercial urease test strips. Notably, the assay required no complex surface modifications or separation procedures, offering high operational simplicity which could be accomplished within 15 min. All these advantages greatly facilitated in-situ and in-time detection of urease. Finally, this assay was applied to the detection of simulated saliva samples with recoveries ranging from 104.4% to 123.0%, thereby validating its robust anti-interference capability and potential application in practice. Thus, the proposed Au@Ag@AgCl core-shell nanostructure-based label-free biosensor might provide a rapid, quantitative, and user-friendly alternative approach for monitoring Heliobacter pylori infection. (© 2025. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.) |
| Competing Interests: | Declarations. Competing interests: The authors declare no competing interests. |
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| Grant Information: | 22376218, 22476214 the National Natural Science Foundation of China; ZR2022JQ07 the Outstanding Youth Fund of Shandong Provincial Natural Science Foundation; 202503024CX the College Student Innovation Project; 25CX02013A the Fundamental Research Funds for the Central Universities |
| Contributed Indexing: | Keywords: Colorimetric ammonia sensing; Core-shell nanostructure; LSPR; Urease detection |
| Substance Nomenclature: | EC 3.5.1.5 (Urease) 7440-57-5 (Gold) 0 (Silver Compounds) MWB0804EO7 (silver chloride) 3M4G523W1G (Silver) |
| Entry Date(s): | Date Created: 20251101 Date Completed: 20251101 Latest Revision: 20251111 |
| Update Code: | 20260130 |
| DOI: | 10.1007/s00604-025-07649-1 |
| PMID: | 41175148 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1436-5073 |
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| DOI: | 10.1007/s00604-025-07649-1 |